The Role of Humoral Antibodies in Rejection of Skin Homografts in Rabbits

Kretschmer, R; Pérez-Tamayo, Ruy

doi:10.1084/jem.116.6.879

Cited by 26 publications

(4 citation statements)

References 61 publications

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“…In addition, passive transfer of contact and tuberculin sensitivities was not accomplished when sensitized lymphoid cells were enclosed within cell-impenetrable Millipore chgmbers and transferred to non-sensitized hosts (6). In contrast, in passive transfer of transplantation immunity, the labeled sensitized ceils were rarely found at the site of homograft rejection and were effective when transferred in Millipore chambers to mice (2), guinea pigs (3), and rabbits (7). Therefore, it would appear that the immunologic mechanisms of tuberculin and contact hypersensitivity differed from that of homograft rejection.…”

Section: Discussionmentioning

confidence: 99%

Passive Transfer of Contact Sensitivity by Tritiated Thymidine-Labeled Lymphoid Cells

Najarían

Feldman

1963

The Journal of Experimental Medicine

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Passive transfer of tritiated thymidine-labeled lymphoid cells sensitized to the simple chemical DNFB into homologous guinea pigs resulted in positive contact skin reactions 24 hours after skin testing with DNFB. Labeled sensitized cells were found to accumulate at these sites, whereas, labeled nonsensitized lymphoid cells did not appear non-specifically in contact skin reaction sites. The labeled cells were small and large lymphocytes and immature cells of the lymphoid series. The maximum reactions were obtained at 24 hours, with an average of 3.4 per cent of the infiltrating mononuclear cells showing a label. At 48 hours, the macro- and microscopic reactions were similar to the 24 hour reactions but diminished in intensity, and the number of labeled cells in the infiltrates had decreased to 1 per cent of the total infiltrating mononuclear cells. ¼ to ⅓ of the labeled cells were found within the epidermis in the test skin sites. These data have indicated that contact sensitivity, like tuberculin sensitivity, required the sensitized cell to initiate the skin reaction and that the majority of the cellular infiltrate was the result of non-specific host response to injury.

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Section: Discussionmentioning

confidence: 99%

Passive Transfer of Contact Sensitivity by Tritiated Thymidine-Labeled Lymphoid Cells

Najarían

Feldman

1963

The Journal of Experimental Medicine

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“…96 Rejection was also induced by sensitized lymph node cells held away from skin grafts by placing them within diffusion chambers, a strong hint toward the role of antibody-mediated injury. 97 In 1963, Altman et al 98 showed cytotoxic antibodies appearing after skin or kidney transplantation in dogs. In their experiments, they could also Azathioprine became available as immunosuppressant in humans.…”

Section: Development and Application Of T Cell-directed Immunosuppres...mentioning

confidence: 99%

“…Indeed, the transfer of alloantibodies or of cells producing them was shown to trigger rejection in experimental models. 96,97 Conversely, successful attempts to counteract AMR via depletion of antibodies or inhibition of their production would be a clinical breakthrough. In 1970, in a xenotransplant model, Bier et al 172 were the first to demonstrate that plasmapheresis could impact the intensity of AMR.…”

Section: Proof Of Concept—antibody Depletion To Counteract Amrmentioning

confidence: 99%

On a Long and Winding Road: Alloantibodies in Organ Transplantation

Böhmig

Halloran

Feucht³

2023

Transplantation

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Today we know that both the humoral and the cellular arm of the immune system are engaged in severe immunological challenges. A close interaction between B and T cells can be observed in most “natural” challenges, including infections, malignancies, and autoimmune diseases. The importance and power of humoral immunity are impressively demonstrated by the current coronavirus disease 2019 pandemic. Organ transplant rejection is a normal immune response to a completely “artificial” challenge. It took a long time before the multifaceted action of different immunological forces was recognized and a unified, generally accepted opinion could be formed. Here, we address prominent paradigms and paradigm shifts in the field of transplantation immunology. We identify several instances in which the transplant community missed a timely paradigm shift because essential, available knowledge was ignored. Moreover, we discuss key findings that critically contributed to our understanding of transplant immunology but sometimes developed with delay and in a roundabout way, as was the case with antibody-mediated rejection—a main focus of this article. These include the discovery of the molecular principles of histocompatibility, the recognition of the microcirculation as a key interface of immune damage, the refinement of alloantibody detection, the description of C4d as a footmark of endothelium-bound antibody, and last but not least, the developments in biopsy-based diagnostics beyond conventional morphology, which only now give us a glimpse of the enormous complexity and pathogenetic diversity of rejection.

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“…The respective roles played by the humoral and cellular components and the possible synergistic or antagonistic actions of each still remain to be clarified. The two sets of experimen tal observations in regard to the destruction of a wide variety of normal and neoplastic cells by, on the one hand, isoantibody and complement or, on the other hand, sensitized lymphoid cells have to some extent been reconciled by K retchmer and Perez-T amayo (18) and N ajarian and Feldman (25) who have shown that sensitized lymphoid cells when placed within diffusion chambers continued to release antibody which was shown to be effective in rejecting the corresponding homografts.…”

Section: (Mm2) There Were 8 Animals In Each Test Group • ----------mentioning

confidence: 99%

Studies on the Cytolytic Effect of Sensitized Lymphoid Cells and Immune Sera in vivo and in vitro

Phillips¹

1967

Int Arch Allergy Immunol

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The Role of Humoral Antibodies in Rejection of Skin Homografts in Rabbits

Cited by 26 publications

References 61 publications

Passive Transfer of Contact Sensitivity by Tritiated Thymidine-Labeled Lymphoid Cells

Passive Transfer of Contact Sensitivity by Tritiated Thymidine-Labeled Lymphoid Cells

On a Long and Winding Road: Alloantibodies in Organ Transplantation

Studies on the Cytolytic Effect of Sensitized Lymphoid Cells and Immune Sera in vivo and in vitro

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