The role of human serum and solution chemistry in fibrinogen peptide–nanoparticle interactions

Zapata, Angela; Nguyen, Mai-Loan; Ling, Caleb; Rogers, James D.; Domiano, Sangeetha; Hayzelden, Clive; Wheeler, Korin E.

doi:10.1039/c9na00793h

Cited by 4 publications

(3 citation statements)

References 62 publications

(130 reference statements)

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“…The affinity of the S material with the fibrinogen promoted its incorporation into the fibrin network. This is because the fibrinogen can be adsorbed on the siliceous surface by electrostatic interaction between the positively charged amino acids (i.e., Lys and Arg) and deprotonated silanol groups at near-neutral pH of the human plasma [ 39 ]. This adsorption occurred mainly by the disordered α C-chains and fibrinogen strands on the surface [ 40 ], which could be beneficial for their polymerization and crosslinking because the presence of particles did not interfere with the fibrin network formation [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

Improving Fibrin Hydrogels’ Mechanical Properties, through Addition of Silica or Chitosan-Silica Materials, for Potential Application as Wound Dressings

Becerra

Múnera

Galeano

et al. 2021

International Journal of Biomaterials

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Fibrin is a protein-based hydrogel formed during blood coagulation. It can also be produced in vitro from human blood plasma, and it is capable of resisting high deformations. However, after each deformation process, it loses high amounts of water, which subsequently makes it mechanically unstable and, finally, difficult to manipulate. The objective of this work was to overcome the in vitro fibrin mechanical instability. The strategy consists of adding silica or chitosan-silica materials and comparing how the different materials electrokinetic-surface properties affect the achieved improvement. The siliceous materials electrostatic and steric stabilization mechanisms, together with plasma protein adsorption on their surfaces, were corroborated by DLS and ζ-potential measurements before fibrin gelling. These properties avoid phase separation, favoring homogeneous incorporation of the solid into the forming fibrin network. Young’s modulus of modified fibrin hydrogels was evaluated by AFM to quantitatively measure stiffness. It increased 2.5 times with the addition of 4 mg/mL silica. A similar improvement was achieved with only 0.7 mg/mL chitosan-silica, which highlighted the contribution of hydrophilic chitosan chains to fibrinogen crosslinking. Moreover, these chains avoided the fibroblast growth inhibition onto modified fibrin hydrogels 3D culture observed with silica. In conclusion, 0.7 mg/mL chitosan-silica improved the mechanical stability of fibrin hydrogels with low risks of cytotoxicity. This easy-to-manipulate modified fibrin hydrogel makes it suitable as a wound dressing biomaterial.

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Section: Resultsmentioning

confidence: 99%

Improving Fibrin Hydrogels’ Mechanical Properties, through Addition of Silica or Chitosan-Silica Materials, for Potential Application as Wound Dressings

Becerra

Múnera

Galeano

et al. 2021

International Journal of Biomaterials

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“…For example, although minor blood pH variations and increases in glucose concentration do not affect the binding of gamma-FBG peptides on SiO 2 NPs, increasing the ionic strength weakens their binding by shielding electrostatic interactions. 92 Protein-triggered aggregation of SiO 2 NPs is dependent on both ionic strength and pH, as determined using lysozyme (Lyz) as a model (Fig. 3).…”

Section: B Effect Of Ph and Ionic Strengthmentioning

confidence: 99%

Nanoparticle protein corona: from structure and function to therapeutic targeting

et al. 2023

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“…At the same time, the content of other proteins in the protein corona also varies with different material properties and surrounding environments. For example, for liposomes, the material composition of liposomes, surface hydrophilicity, hydrophobicity, and target molecular modification are the most influential factors on the surface protein corona composition of liposomes after entering the blood circulation; for mesoporous silica nanoparticles (MSN), the shape and pore size will affect the conformation of adsorbed albumin and fibrinogen, and the concentration of glucose in the blood will increase the amount of adsorbed fibrinogen …”

Section: Material-related In Vivo Reaction Studymentioning

confidence: 99%

Intravenous Hemostats: Foundation, Targeting, and Controlled-Release

et al. 2022

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Uncontrollable blood loss is the greatest cause of mortality in prehospital patients and the main source of disability and death in hospital care. Compared with external hemostats, intravenous hemostats are more appropriate for preventing and treating uncontrolled bleeding in vivo and large bleeding on the body surface. This Review initially establishes intravenous hemostats' response basis, including the coagulation mechanism, fibrinolytic pathway, and protein corona. Second, the study of advancement of intravenous hemostat targeting was expanded from two perspectives, cellular hemostatic agents and synthetic hemostatic agents. Meanwhile, after discussing the progress of controlled-release intravenous hemostats with platelets as the stimuli, this Review offers insight into the possibility of controlled-release intravenous hemostats with microenvironment as the stimuli, combining the studies of controlled-release targeted thrombolysis.

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The role of human serum and solution chemistry in fibrinogen peptide–nanoparticle interactions

Abstract: In living systems, biomolecule–nanoparticle interactions are mediated by proteins, like those in human serum, and by solutes.

Cited by 4 publications

References 62 publications

Improving Fibrin Hydrogels’ Mechanical Properties, through Addition of Silica or Chitosan-Silica Materials, for Potential Application as Wound Dressings

Improving Fibrin Hydrogels’ Mechanical Properties, through Addition of Silica or Chitosan-Silica Materials, for Potential Application as Wound Dressings

Nanoparticle protein corona: from structure and function to therapeutic targeting

Intravenous Hemostats: Foundation, Targeting, and Controlled-Release

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