The Role of HSP90 Chaperones in Stability and Plasticity of Ontogenesis of Plants under Normal and Stressful Conditions (Arabidopsis thaliana)

Kozeko, Liudmyla

doi:10.3103/s0095452719020063

Cited by 12 publications

(8 citation statements)

References 110 publications

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“…In Arabidopsis , it was found out that under normal conditions heat shock transcription factors (HSF) are associated with the Hsp90/Hsp70 complex. During the stress, the chaperones switch to the binding of damaged proteins and release HSF, which trigger gene expression of Hsp and several HSF (Kozeko 2019). In our experiment, intense ~150 kDa protein apparent in all plant groups mainly at 1 h and 1 day could correspond to Hsp90/Hp70 complex.…”

Section: Discussionmentioning

confidence: 99%

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Effects of heat treatment on metabolism of tobacco plants infected with Potato virus Y

et al. 2021

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Many factors affect successful virus propagation and plant defence responses. Heat shock protein (Hsp) expression after heat shock plays an ambiguous role in viral infection. On the one hand, Hsp70 participates in plant defence response; on the other hand, Hsp70 could interact with viral proteins and facilitate virus propagation.• Here, we studied metabolic adaptations of Nicotiana tabacum L. subjected to heat shock (42°C, 2 h) before or after inoculating the plants with Potato virus Y (potyvirus). RT-qPCR and ELISA were used for potyvirus quantification. Hsp70 and Hsp90 isoforms were analysed by Western blotting. Salicylic, quinic and chlorogenic acid content was determined by LC-MS. The activity of Hatch-Slack enzymes (as markers of potyviral infection in tobacco) and glycosidases was assayed.• Application of heat shock before or after inoculation showed accelerated potyviral propagation in comparison with only inoculated plants. Plants exposed to heat shock and concurrently inoculated showed higher potyviral content, higher amount of Hsp70, together with late decline of quinic acid content and low chlorogenic acid content. Spread of potyviral infection correlated with enhanced salicylic acid content and activities of enzymes of the Hatch-Slack cycle, aand b-galactosidase, a-mannosidase, a-glucosidase and b-N-acetylhexosaminidase.• Heat shock proteins accelerate potyviral propagation. The lower weight cytosolic and mitochondrial Hsp70 (~50-75 kDa) persist throughout the viral infection. Also, the plant defense response results in increase of salicylic and chlorogenic acids but decrease of quinic acid content.

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Section: Discussionmentioning

confidence: 99%

“…In addition, Hsp90 determines functioning of several complicated protein complexes, including 26S proteasome, ribosomal subunit 40S, RNA‐induced silencing complex, etc. (Kozeko 2019).…”

Section: Introductionmentioning

confidence: 99%

Effects of heat treatment on metabolism of tobacco plants infected with Potato virus Y

et al. 2021

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“…They associate with the suppressor of the G2 allele of skp1 ( SGT1 ) for the stabilization of the nucleotide-binding domain and leucine-rich repeat-containing (NLR) immune sensors, which mediate plant defense mechanisms [29]. In addition, HSP90s participate in stress signaling pathways [18,30,31,32,33]. In fact, under stress, the inhibition of HSP90 induces, in most cases, deregulation of the putative client’s functionality and alteration of cell processes [31,34].…”

Section: Introductionmentioning

confidence: 99%

Genetically Modified Heat Shock Protein90s and Polyamine Oxidases in Arabidopsis Reveal Their Interaction under Heat Stress Affecting Polyamine Acetylation, Oxidation and Homeostasis of Reactive Oxygen Species

Toumi

Pagoulatou

Margaritopoulou

et al. 2019

Plants

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The chaperones, heat shock proteins (HSPs), stabilize proteins to minimize proteotoxic stress, especially during heat stress (HS) and polyamine (PA) oxidases (PAOs) participate in the modulation of the cellular homeostasis of PAs and reactive oxygen species (ROS). An interesting interaction of HSP90s and PAOs was revealed in Arabidopsis thaliana by using the pLFY:HSP90RNAi line against the four AtHSP90 genes encoding cytosolic proteins, the T-DNA Athsp90-1 and Athsp90-4 insertional mutants, the Atpao3 mutant and pharmacological inhibitors of HSP90s and PAOs. Silencing of all cytosolic HSP90 genes resulted in several-fold higher levels of soluble spermidine (S-Spd), acetylated Spd (N8-acetyl-Spd) and acetylated spermine (N1-acetyl-Spm) in the transgenic Arabidopsis thaliana leaves. Heat shock induced increase of soluble-PAs (S-PAs) and soluble hydrolyzed-PAs (SH-PAs), especially of SH-Spm, and more importantly of acetylated Spd and Spm. The silencing of HSP90 genes or pharmacological inhibition of the HSP90 proteins by the specific inhibitor radicicol, under HS stimulatory conditions, resulted in a further increase of PA titers, N8-acetyl-Spd and N1-acetyl-Spm, and also stimulated the expression of PAO genes. The increased PA titers and PAO enzymatic activity resulted in a profound increase of PAO-derived hydrogen peroxide (H2O2) levels, which was terminated by the addition of the PAO-specific inhibitor guazatine. Interestingly, the loss-of-function Atpao3 mutant exhibited increased mRNA levels of selected AtHSP90 genes. Taken together, the results herein reveal a novel function of HSP90 and suggest that HSP90s and PAOs cross-talk to orchestrate PA acetylation, oxidation, and PA/H2O2 homeostasis.

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“…Famili dari protein HSP90 terlibat dalam replikasi dan perbaikan DNA, homeostasis seluler, agregasi protein folding serta mencegah denaturasi termal (Boston et al, 1996;Li et al, 2012). Protein ini juga berperan dalam pengaturan sinyal transduksi hormon brassinosteroids (BR), auksin, dan asam jasmonat (Li et al, 2012;Kozeko, 2019). Protein HSP90 terlibat dalam pengaturan siklus sel serta bertindak sebagai protein trafficking.…”

Section: Hsp90unclassified

“…Protein HSP90 terlibat dalam pengaturan siklus sel serta bertindak sebagai protein trafficking. Selain itu, juga berperan dalam stabilitas morfogenesis, plastisitas perkembangan, dan mekanisme toleransi stres pada tanaman (Kozeko, 2019). HSP90 dilaporkan sebagai pengatur utama pertumbuhan dan perkembangan normal pada Nicotiana benthamiana dan Arabidopsis (Kadota & Shirasu, 2012).…”

Section: Hsp90unclassified

Peran Chaperone Pada Tumbuhan: Mini Review

2019

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Plants respond to various conditions in the surrounding environment, whether favorable conditions or vice versa. Abiotic and biotic factors affect plant responses such as temperature, humidity, salinity, insects and pathogens such as viruses and bacteria. Plants have a defense system in tolerancing stress from the surrounding environment, for example heat shock protein (HSP) is a chaperone protein that plays a role in plant defenses when experiencing stress to the temperature. HSP is classified into six families based on their molecular weight, namely HSP100, HSP90, HSP70, HSP60, HSP40, and small HSP. Each has a role in maintaining the stability of plant metabolism. HSP is especially important for correct protein refolding, preventing degradation and denaturation of protein. Key words: plants; chaperone; heat shock protein; refolding; protein denaturation.

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The Role of HSP90 Chaperones in Stability and Plasticity of Ontogenesis of Plants under Normal and Stressful Conditions (Arabidopsis thaliana)

Cited by 12 publications

References 110 publications

Effects of heat treatment on metabolism of tobacco plants infected with Potato virus Y

Effects of heat treatment on metabolism of tobacco plants infected with Potato virus Y

Genetically Modified Heat Shock Protein90s and Polyamine Oxidases in Arabidopsis Reveal Their Interaction under Heat Stress Affecting Polyamine Acetylation, Oxidation and Homeostasis of Reactive Oxygen Species

Peran Chaperone Pada Tumbuhan: Mini Review

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