The role of honeybee pollen as a natural source of antioxidants in the in vitro maturation medium of sheep oocytes and its effect on gene expression

Barakat, Ibrahim A. H.; Kaabi, Aaishah M.; Alajmi, Reem A.

doi:10.1007/s11356-020-09386-9

Cited by 2 publications

(4 citation statements)

References 45 publications

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“…The data of the current study were in line with our previous study (Barakat et al 2020) and that of Kaabi et al (2020), who used honeybee pollen and black seed natural honey (N. sativa) in the IVM medium of sheep oocytes.…”

Section: Discussionsupporting

confidence: 90%

“…Many investigations have reported that bee products, such as honey and honeybee pollen, are rich in nutrients and antioxidants. Therefore, supplementing the IVM medium with honeybee pollen or honey is a possible way to improve the IVM efficiency (Barakat et al 2020;Kaabi et al 2020). Honey is a complex biological substance produced by Apis mellifera bees and composed mainly from vitamins (e.g., retinol, tocopherol, anti-haemorrhagic vitamin, thiamine), amino acids (e.g., glutamic acid, arginine, cysteine, aspartic acid, and proline), fatty acids, flavonoids, sugars, phenolic compounds, mineral salts, carotenoid contents, and antimicrobial and antioxidant substances (Abel and Baird 2018).…”

mentioning

confidence: 99%

“…Kaabi et al (2020) has reported that the addition of black seed honey (5%) to the maturation medium of sheep oocytes improved the maturity and gene expression (GDF-9, MPF, CMOS, IGF-1 and BAX) and enhanced the intracellular glutathione concentration. Barakat et al (2020) have demonstrated that the use of honeybee pollen at a concentration of 1 μg/ml as a supplement in an IVM medium improved the in vitro maturation rate of sheep oocytes, increased the glutathione concentration, and enhanced the gene expression.…”

mentioning

confidence: 99%

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Ameliorative effect of bee products on in vitro maturation of sheep oocytes

Kaabi¹,

Barakat²,

Alajmi³

et al. 2022

Vet. Med. - Czech

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The present study was undertaken to investigate the possible stimulating effects of Nigella sativa (N. sativa) honey, natural Saudi Sider honey, and honeybee pollen to the in vitro maturation (IVM) medium of sheep oocytes on their subsequent development. Hence, immature oocytes were exposed to various concentrations of natural Nigella sativa (N. sativa), and Saudi Sider honey (5, 10, and 20%), as well as honeybee pollen (1, 10, 50 μg/ml) during an in vitro maturation period (24 hours). After the exposure time, the maturation rate, glutathione (GSH) concentration, and candidate gene expression (GDF-9, MPF, CMOS, IGF-1, and BAX) were evaluated. Our results showed that the maturation rate was higher in the groups challenged with the lowest level of the bee products (5% and 1 μg/ml) when compared with that in the control group; where the mean number of oocytes in the metaphase II stage reached 0.360 for the honeybee pollen-treated group, 0.293 for the N. sativa-treated group, and 0.203 for the natural Saudi Sider honey-treated group. The glutathione level was significantly increased in the group exposed to N. sativa honey when compared with the other groups. Concerning the gene expression results, the Saudi Sider honey treatment showed the best results for all the genes except the CMOS gene, which was significantly higher than the GI and GII groups and lower than the GIV group and the BAX gene which did not show a significant difference when compared with the other groups. In conclusion, the addition of natural honey and honeybee pollen at a low concentration to an IVM medium improved the in vitro maturation rate, increased the glutathione level, and gene expression of the in vitro matured ovine oocytes.

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Section: Discussionsupporting

confidence: 90%

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confidence: 99%

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confidence: 99%

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Ameliorative effect of bee products on in vitro maturation of sheep oocytes

Kaabi¹,

Barakat²,

Alajmi³

et al. 2022

Vet. Med. - Czech

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“…In recent years, there have been several uses of bee products in both traditional and modern medicine, which include honey, royal jelly, bee pollen, beebread, bee venom, and propolis (Molan 1999;Veshkini et al 2018). Recent research has reported that the addition of Nigella sativa honey and honeybee pollen to the in vitro maturation medium of sheep oocytes increased both the maturation rate and gene expression and enhanced the GSH content (Barakat et al 2020;Kaabi et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Gene Expression and In Vitro Maturation of Sheep Oocytes using Bee Pollen and Bee Honey as Medium Supplements

Kaabi¹

2021

IJAB

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This study was conducted to investigate the effects of raw honey obtained from black seed or Sider and honeybee pollen as an additive in sheep oocyte maturation medium on the oocyte maturation rate, changes in oocyte glutathione (GSH) levels and expression of developmental candidate genes (GDF-9, MPF, C-MOS, IGF-1, BAX). Healthy immature oocytes of Najdi sheep were cultured in a medium supplemented with 5.0% Sider or Nigella sativa (black seed) honey + 1.0 μg/mL honeybee pollen, and after 24 h of incubation, the effects on the improvement of in vitro oocyte maturation were evaluated. Results demonstrated that the mean oocyte maturation rate was the best in group treated with 5% N. sativa (Group 3) compared with group treated with Sider or N. sativa honey (Group 1A and B, respectively). Mean GSH level was higher in Group 3 oocytes (11.09 ± 0.29 nmol) than in Group 2 oocytes (honey alone; 10.93 ± 0.57; P ≤ 0.05). Mean GSH levels were significantly decreased in Group 1. Expression analysis of candidate genes showed significant upregulation of GDF-9, cyclin B, C-MOS and IGF 1 genes in Group 3 and downregulation of BAX compared with control Group 1. In conclusion, addition of 1.0 μg/mL honeybee pollen along with one of two types of bee honey (Sider and N. sativa) at 5% concentration to the in vitro maturation medium of Najdi sheep oocytes has a beneficial effect in improving the maturation rate and gene expression and increasing the glutathione concentration in matured oocytes. © 2020 Friends Science Publishers

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The role of honeybee pollen as a natural source of antioxidants in the in vitro maturation medium of sheep oocytes and its effect on gene expression

Cited by 2 publications

References 45 publications

Ameliorative effect of bee products on in vitro maturation of sheep oocytes

Ameliorative effect of bee products on in vitro maturation of sheep oocytes

Gene Expression and In Vitro Maturation of Sheep Oocytes using Bee Pollen and Bee Honey as Medium Supplements

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