The Role of High Fluorescent Reticulocytes in Monitoring the Aplasia Outcome and Optimizing the Timing of Peripheral Blood Stem Cell Harvesting

Lesesve, Jean-Franç Ois; Lenormand, Bernard; Lacombe, Francis

doi:10.1089/152581602321080664

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…Prolonged survival of younger reticulocytes followed by reticulocyte death would result in a normal/low reticulocyte count (Lesesve, Lenormand & Lacombe, 2002). In cases of stressed erythropoiesis, a left shift of the reticulocyte differential would lead to HFR release in numbers too small to modify/increase the total absolute reticulocyte count, but that could be detected by current automated analysers.…”

Section: Reticulocyte Measurements In Myelodysplastic Syndrome Patienmentioning

confidence: 99%

Increase of immature reticulocyte fraction in myelodysplastic syndromes

Lesesve¹,

Daliphard²,

Callat³

et al. 2004

Clin Lab Haematol

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Section: Reticulocyte Measurements In Myelodysplastic Syndrome Patienmentioning

confidence: 99%

Increase of immature reticulocyte fraction in myelodysplastic syndromes

Lesesve¹,

Daliphard²,

Callat³

et al. 2004

Clin Lab Haematol

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“…Considering that the fluorescence intensity is directly proportional to the amount of RNA, thus reflecting the immaturity of the cells, RMI was proven to be useful for the assessment of both bone marrow engraftment and erythropoietic activity as well as for anaemia classifications (Ware, Rosse & Hall, 1995; Davis, Ornvold & Bigelow, 1995; Davis et al. , 1989; Lesesve, Lenormand & Lacombe, 2002). ADVIA 120 and the newer ADVIA 2120 (SIEMENS) haematological analysers have introduced an innovative method to separate reticulocyte populations on the basis of cell volume and absorbance of laser light (after exposure to the dye Oxazine 750).…”

Section: Introductionmentioning

confidence: 99%

A biparametric flow cytometry analysis for the study of reticulocyte patterns of maturation

Borrione

Spaccamiglio²,

Parisi³

et al. 2010

Int J Lab Hematology

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Automated haematological analysers still represent the gold standard for the study of reticulocyte maturation even if this technique is based on structural properties and staining affinity rather than on functional aspects. On the contrary, flow cytometry allows the simultaneous analysis of multiple cellular characteristics including functional features. Aim was to investigate whether simultaneous analysis of different reticulocyte parameters using flow cytometry may add functional information when considering their pattern of maturation. Thirty-nine healthy donors (H) and 31 haemodialysed patients on treatment with rHuEpo (HDT) were analysed. Reticulocyte counts and their stages of maturation were studied both with ADVIA 2120 and by flow cytometry. TO/CD71 scattergraph reticulocyte analysis designed a peculiar distribution which was similar among the same group of subjects (H or HDT), but different between H and HDT. distribution of the percentage of reticulocytes in low, medium and high boxes calculated by ADVIA 2120 did not show any difference between H and HDT groups, while the analysis using flow cytometry pointed out statistically significant differences between H and HDT groups in the three boxes where the TO+/CD71+ reticulocytes were localized. The present study suggests that TO/CD71 analysis was reproducible and could detect different pattern of maturation of a particular clinical setting.

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The Role of High Fluorescent Reticulocytes in Monitoring the Aplasia Outcome and Optimizing the Timing of Peripheral Blood Stem Cell Harvesting

Cited by 2 publications

References 7 publications

Increase of immature reticulocyte fraction in myelodysplastic syndromes

Increase of immature reticulocyte fraction in myelodysplastic syndromes

A biparametric flow cytometry analysis for the study of reticulocyte patterns of maturation

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