The role of heparan sulphate‐binding activity of Helicobacter pylori bacteria in their adhesion to murine macrophages

Abstract

M., Paziak-Domanska, B., Rudnicka, W. & Wadstrom, T. The role of heparan sulphatebinding activity of Helicobacter pylori bacteria in their adhesion to murine macrophages. APMIS 103: 469474, 1995.The aim of this study was to determine the role of heparan sulphate (HS)-binding activity of Helicobucter pylori microbes in their adhesion to and ingestion by inflammatory peritoneal macrophages. Two H . pylori strains expressing sialic acid-specific haemagglutinins but differing in the expression of heparan sulphate-binding capacity were chosen for investigation. The attachment to an ingestion by macrophages of the H. pylori bacteria were estimated by ELISA using anti-H. pylori antibodies. The adhesion of both H. pylori strains could be inhibited by pretreatment of the bacteria with heparin (H), HS or fetuin, as well as by preincubation of the macrophages with heparinase or neuraminidase. However, detailed analysis of the data on the inhibition of bacterial adhesion to macrophages led to the conclusion that the attachment of H. pylori 25 bacteria, which expressed a high heparan sulphate binding, was mainly determined by HS-binding structures. In contrast, the adhesion to macrophages of H. pylori bacteria 17874 microbes, which expressed a weak heparan sulphate binding, was more dependent on the exhibition of sialic acid-dependent haemagglutinins. The described variation in H. pylori bacterial surface structures mediating their adhesion to macrophages could suggest a similar variation in bacterial adhesion to stomach mucosa and maybe in the pathogenicity of H. pylori strains.

Effect of Heparin, Fucoidan and Other Polysaccharides on Adhesion of Enterohepatic Helicobacter Species to Murine Macrophages

“…According to previously published data, heparin showed optimal inhibition of H pylori adhesion at a concentration of 1 mg/ml [10]. FITC labelled bacterial suspensions was mixed with equal volumes of RPMI alone or with polysaccharides at a concentration of 1.25 mg/ml, agitated for 1 h at 37°C and added to a macrophage monolayer in relation 50 CFU per one macrophage.…”

“…In the present study we chose the murine macrophage cell line J774A.1 since H. pylori was shown to adhere strongly to these cells [9,10]. The macrophages were inoculated with EHS pretreated with different polysulphated agents (heparin, heparan sulphate, chondroitin sulphate, dextran sulphate and fucoidan).…”

Stopping Bacterial Adhesion: A Novel Approach to Treating Infections

“…H. pylori binding is complex and has been comprehensively reviewed by Karlsson [137]; binding ligands include 10 separate carbohydrate specificities. Binding to sialic acid [59], sulphate lipids [138], heparan sulphate [67, 139], fucose [140,141], mucin [142], ganglio glycolipids [143], lactosylceramide [144], monhexylceramides [145], glycosphingolipids [146], phosphlipid, phosphatidylethanolamine [147], Lewis b oligosaccharide [148] and blood group antigens [149, 150] has been demonstrated.…”

Involvement of the heparan sulphate-binding proteins of Helicobacter pylori in its adherence to HeLa S3 and Kato III cell lines

“…Such phenomena may in part explain why H. pylori is a slow pathogen [17,18] that can survive in the gastric mucosa for decades in patients with chronic type B gastritis. Chmiela et al [19] demonstrated that H. pylori also used heparan sulphate-binding activity in its adhesion to murine peritoneal macrophages.…”