The role of heparan sulfate on adhesion of 47 and 51 kDa outer membrane proteins ofHelicobacter pylorito gastric cancer cellsThis study was done in memory of the late Dr. Roberto Carlos Vazquez-Juarez.

Abstract

Helicobacter pylori is a common gastrointestinal pathogenic bacterium in humans and the usual preference for the stomach's outer membrane proteins (OMPs) are antigens involved in the adhesion process. Through SDS-PAGE and blotting analyses, using horseradish peroxidase-labeled heparan sulfate (HRP-HS) as a probe, we identified H. pylori OMPs with affinity for heparan sulfate (OMP-HS). Biotin-streptavidin bacterial-adhesion assay was used to evaluate participation of OMP-HS in the adhesion of H. pylori to semi-confluent HeLa S3 and Kato III cell monolayers. The results provide evidence that induction of antibodies against 2 OMP-HSs (HSBP-47 and HSBP-51) could reduce binding of H. pylori to both cell lines and induce detachment of cell-bound bacteria from infected cultured cells.

Helicobacter pylori adhesion to gastric epithelial cells is mediated by glycan receptors

“…Additionally, two sulfo-binding H. pylori proteins have been described, the neutrophil-www.bjournal.com.br Braz J Med Biol Res 43 (7) 2010 activating protein (NAP), which binds specifically to sulfated oligosaccharide structures such as sulfo-Le a , sulfogalactose and sulfo-N-acetyl-glucosamine on mucins, and Hsp70 that has been suggested to mediate sulfatide recognition under stress conditions (49,50). Heparan sulfate glycosaminoglycans have also been described to have a role in H. pylori adhesion to cell-line models (51,52). In vitro studies have shown that H. pylori infection up-regulates expression of syndecan-4, which represents one of the major sources of heparan-sulfate on the cell surface (53,54).…”