The role of Helicobacter pylori urease in the pathogenesis of gastritis and peptic ulceration.

Mobley, Harry L. T.

doi:10.1046/j.1365-2036.1996.22164006.x

Cited by 143 publications

(99 citation statements)

References 9 publications

(11 reference statements)

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“…Integrins are highly conserved and are reported to play a key role in cell migration, cell-cell adhesion, cell-extracellular matrix adhesion, proliferation, phagocytosis, apoptosis and differentiation (Kwok et al, 2007). UreG is one member of the Ure gene cluster, and it encodes an accessory protein required for nickel ion insertion into the apoenzyme (Mobley, 1996;Mao and Yan, 2004). In the biosynthesis of the active metal-bound form of the nickel-dependent enzyme urease, a lysinecarbamate functional group is formed alongside with the delivery of two Ni (2+) ions into the precast active site of the apoenzyme.…”

Section: Discussionmentioning

confidence: 99%

Antigenic Proteins of Helicobacter pylori of Potential Diagnostic Value

Khalilpour

Agilan²,

Lee³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Helicobacter pylori antigen was prepared from an isolate from a patient with a duodenal ulcer. Serum samples were obtained from culture-positive H. pylori infected patients with duodenal ulcers, gastric ulcers and gastritis (n=30). As controls, three kinds of sera without detectable H. pylori IgG antibodies were used: 30 from healthy individuals without history of gastric disorders, 30 from patients who were seen in the endoscopy clinic but were H. pylori culture negative and 30 from people with other diseases. OFF-GEL electrophoresis, SDS-PAGE and Western blots of individual serum samples were used to identify protein bands with good sensitivity and specificity when probed with the above sera and HRP-conjugated anti-human IgG. Four H. pylori protein bands showed good (≥ 70%) sensitivity and high specificity (98-100%) towards anti-Helicobacter IgG antibody in culturepositive patients sera and control sera, respectively. The identities of the antigenic proteins were elucidated by mass spectrometry. The relative molecular weights and the identities of the proteins, based on MALDI TOF/ TOF, were as follows: CagI (25 kDa), urease G accessory protein (25 kDa), UreB (63 kDa) and proline/pyrroline-5-carboxylate dehydrogenase (118 KDa). These identified proteins, singly and/or in combinations, may be useful for diagnosis of H. pylori infection in patients.

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Section: Discussionmentioning

confidence: 99%

Antigenic Proteins of Helicobacter pylori of Potential Diagnostic Value

Khalilpour

Agilan²,

Lee³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…2A). UreaseA, a major protein of H. pylori that is found both in the bacterial cytosol and associated with the outer bacterial membrane (24), was used as a control to show that the bacteria were not lysed during the fractionation procedure. Fig.…”

Section: Induction Of the Scattering Phenotype Il-8 Release And Coxmentioning

confidence: 99%

Subcellular and Functional Proteomic Analysis of the Cellular Responses Induced by Helicobacter pylori

Chan

Chang

et al. 2006

Molecular & Cellular Proteomics

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Helicobacter pylori infection is a crucial factor in the pathogenesis of several digestive disorders, including peptic ulcers, chronic gastritis, and gastric cancer. Moreover H. pylori induces disease-specific protein expression in gastric epithelial cells. The aim of the present study was to characterize proteins differentially expressed in H. pylori-infected gastric epithelial AGS cells. An in vitro model was established using a multiplicity of infection of 100 and evaluating the effectiveness of H. pylori infection by functional analyses. Changes in protein patterns were identified using a proteomic approach consisting of two-dimensional fluorescence difference gel electrophoresis and mass spectrometry. The expression of many proteins was found to be altered, and 28 of these were identified and classified as protein synthesis-and folding-related proteins, cytoskeleton proteins, metabolic enzymes, transcription-and translation-related proteins, angiogenesis/ metastasis-related proteins, cell communication/signal transduction-related proteins, or others (oxygen-regulated protein and oncoprotein). The expression profiles of eight of these proteins, laminin ␥-1 chain precursor, valosin-containing protein, heat shock 70-kDa protein, mitochondrial matrix protein P1, FK506-binding protein 4, Tcomplex protein 1, enolase ␣, and 14-3-3 ␤ were further examined in cancerous and paired surrounding normal tissues by immunoblot assay and immunohistochemical staining to identify molecular targets that may be involved in the pathogenesis of H. pylori-induced gastric diseases. On the basis of our results, valosin-containing protein, mitochondrial matrix protein P1, T-complex protein 1, enolase ␣, and 14-3-3

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“…1 The bacteria produce urease, which causes tissue damage. 2 Once acquired, the H. pylori infection persists for life if not treated and is responsible for major morbidity and mortality throughout the world. The incidence rate of H. pylori infection in chronic gastritis from various regions of Saudi Arabia ranges from 50% to 96%.…”

Section: Ar Khan An Age-and Gender-specific Analysis Of H Pylori Inmentioning

confidence: 99%

An Age- and Gender-Specific Analysis of H. Pylori Infection

Khan

1998

Ann Saudi Med

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The objective of this study was to determine the incidence of Helicobacter pylori infection in different age and sex groups from the southern region of Saudi Arabia, and to relate the results to the high incidence of gastric cancer in this region. The carcinogenic effect of H. pylori is considered to be age-dependent. H. pylori infection early in life predisposes patients to gastric cancer. In contrast, acquisition of infection later in life leads to development of duodenal ulcer. We studied 528 consecutive endoscopic biopsies over a period from March 1995 to August 1996. The presence or absence of H. pylori was tabulated according to age and sex of the patients. Three hundred and fifty-three (67%) of 528 patients were positive for H. pylori. There were 313 males, among whom 217 (69%) were positive. There were 215 females, among whom 136 (63%) were positive for H. pylori. The presence of H. pylori in various age groups was as follows: under 16 years, 62%; 16 to 25 years, 67%; 26-35 years, 69%; 36-45 years, 67%; 46-55 years, 66%; and 56 years and above, 65%. Statistical analysis revealed no significant difference between the groups. No significant rise in the infection rate was noted from childhood to advanced age. These data support the belief that H. pylori infection was acquired early in life, leading to multifocal gastritis and thus predisposing the patients to gastric cancer later in life. However, H. pylori may not be the single agent of gastric cancer inasmuch as the infection affects both genders equally, whereas gastric cancer has a male preference. Perhaps some additional factors augment the pivotal role of

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The role of Helicobacter pylori urease in the pathogenesis of gastritis and peptic ulceration.

Cited by 143 publications

References 9 publications

Antigenic Proteins of Helicobacter pylori of Potential Diagnostic Value

Antigenic Proteins of Helicobacter pylori of Potential Diagnostic Value

Subcellular and Functional Proteomic Analysis of the Cellular Responses Induced by Helicobacter pylori

An Age- and Gender-Specific Analysis of H. Pylori Infection

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