The Role of Heat Shock Proteins in Response to Extracellular Stress in Aquatic Organisms

Wong, Li Lian; Do, Thinh Dinh

doi:10.1007/978-3-319-73377-7_9

Cited by 11 publications

(6 citation statements)

References 177 publications

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“…ubiquitin carboxyl-terminal hydrolase, proteasome subunits and proteasome regulatory subunits), were also significantly decreased. This result is quite difficult to interpret, because the presence of xenobiotics is known to cause stress at the cellular level, and most of these protein markers (HSPs, oxidative stress proteins and proteasome) are generally described as accumulated under such conditions (Kim & Kang, 2016;Wong & Do, 2017). As said previously, we wondered if the caging by itself could, for different reasons, induce a situation of significant stress for fish.…”

Section: General Perturbation Of the Cell Metabolismmentioning

confidence: 93%

Coupling caging and proteomics on the European flounder (Platichthys flesus) to assess the estuarine water quality at micro scale

Borcier

Artigaud

Gaillard

et al. 2019

Science of The Total Environment

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Estuaries are important areas highly vulnerable to anthropogenic pollutions. Therefore, the assessment of estuarine water quality is a major ecological issue. In this study, we sampled juveniles of the European flounder in the "pristine" Canche estuary, and caged them in Canche and in two polluted sites of the Seine estuary, Rouen and Fosse Nord. After one month, the metal and organic pollutants in these sites were assessed, and we evaluated several phenotypic indicators (condition index, RNA/DNA ratios and genotoxicity), and extracted the proteins in fish livers for analysis using a shotgun proteomics approach.The results showed strong modifications in the fish caged in both sites of the Seine estuary, as compared to those caged in Canche. In particular, many proteins involved in phase I and phase II detoxification reactions were accumulated in the liver of fish caged in the site showing the highest pollution, Rouen. In addition, we observed a general disruption of metabolism, in particular an increase in lipid synthesis and carbohydrate degradation in Rouen, and a decrease in the abundance of proteins associated to translational activity in Fosse Nord. At both sites, several stress proteins were decreased.The proteomic impact of the encagement by itself was also evaluated, by comparing the liver proteome of fish caged in Canche to that of fish stayed in natura during the same time. The results showed proteomic signatures of exposure to stressful conditions (particularly heat stress), most probably related to the microhabitat in which the cages were placed. In conclusion, the caging technique is of great interest for ecotoxicological assessment of estuarine waters, but should consider that the results are representative of the micro-habitat around the cages, which does not necessarily represent the overall heterogeneity of the estuarine environment.

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Section: General Perturbation Of the Cell Metabolismmentioning

confidence: 93%

Coupling caging and proteomics on the European flounder (Platichthys flesus) to assess the estuarine water quality at micro scale

Borcier

Artigaud

Gaillard

et al. 2019

Science of The Total Environment

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“…Lactate is a product of anaerobic glycolysis activated under deficient oxygen supply [25], and its concentration was determined to disclose the possible local occlusions of the hemolymph flow caused by the microcapsules. HSP70 are considered by many authors as a sensitive biomarker that responds to a variety of stressors, including toxic effects and hypoxia [46,47,48]. GST activity and lactate concentrations monitored for two weeks, as well as HSP70 concentration evaluated for one week, showed no reaction (all p > 0.15) to the injections (Figure 4).…”

Section: Resultsmentioning

confidence: 99%

Application of PEG-Covered Non-Biodegradable Polyelectrolyte Microcapsules in the Crustacean Circulatory System on the Example of the Amphipod Eulimnogammarus verrucosus

et al. 2019

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Layer-by-layer assembled microcapsules are promising carriers for the delivery of various pharmaceutical and sensing substances into specific organs of different animals, but their utility in vivo inside such an important group as crustaceans remains poorly explored. In the current study, we analyzed several significant aspects of the application of fluorescent microcapsules covered by polyethylene glycol (PEG) inside the crustacean circulatory system, using the example of the amphipod Eulimnogammarus verrucosus. In particular, we explored the distribution dynamics of visible microcapsules after injection into the main hemolymph vessel; analyzed the most significant features of E. verrucosus autofluorescence; monitored amphipod mortality and biochemical markers of stress response after microcapsule injection, as well as the healing of the injection wound; and finally, we studied the immune response to the microcapsules. The visibility of microcapsules decreased with time, however, the central hemolymph vessel was confirmed to be the most promising organ for detecting the spectral signal of implanted microencapsulated fluorescent probes. One million injected microcapsules (sufficient for detecting stable fluorescence during the first hours after injection) showed no toxicity for six weeks, but in vitro amphipod immune cells recognize the PEG-coated microcapsules as foreign bodies and try to isolate them by 12 h after contact.

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“…HSP is an important protein for cell injury repairing and in guaranteeing normal cell metabolism. It is related not only related to temperature stress, but also to stresses caused by many biotic and abiotic factors (Hagn et al., 2011; Krukenberg, Street, Lavery, & Agard, 2011; Wong & Do, 2017).…”

Section: Discussionmentioning

confidence: 99%

Physiological responses to salinity stress in the Managua Cichlid,Cichlasoma managuense

Chen

Zhong

et al. 2020

Aquac Res

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The present study was designed to examine the physiological responses of juvenile managua cichlid, Cichlasoma managuense under short-term salinity stress, that is transferring C. managuense from freshwater to brackish water of salinity 0 (control group), 3‰, 6‰, 9‰, 12‰ or 15‰ for 48, 96, 144 and 192 hr respectively. The results showed that gill Na + /K +-ATPase (NKA) activity revealed a decreased (48 hr), increased (96 hr) and then slightly decreased (144 hr, 192 hr) response with increasing salinity and the lowest NKA level was consistently detected at 9‰ salinity treatment; gill Ca 2+ /Mg 2+-ATPase (CMA) activities in all salinity treatments were significantly higher than that of the control group (p < .05) before 144 hr; the RNA/ DNA ratio was significantly reduced when exposed to high salinities (≥9‰), suggesting growth inhibition; serum cortisol level gradually decreased at low salinities (up to 6‰) but dramatically increased at high salinities of 12‰ and 15‰; and both serum glucose/lactic acid levels and heat shock protein 90 (HSP90) level in liver/muscle were decreased at the salinity between 9‰ and 6‰ but increased at the salinity between 12‰ and 15‰, which was a U-shape response curve. Taken together, our data suggest that C. managuense can adapt well at salinity up to 0‰-9‰ and high salinity may cause detrimental effects.

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The Role of Heat Shock Proteins in Response to Extracellular Stress in Aquatic Organisms

Cited by 11 publications

References 177 publications

Coupling caging and proteomics on the European flounder (Platichthys flesus) to assess the estuarine water quality at micro scale

Coupling caging and proteomics on the European flounder (Platichthys flesus) to assess the estuarine water quality at micro scale

Application of PEG-Covered Non-Biodegradable Polyelectrolyte Microcapsules in the Crustacean Circulatory System on the Example of the Amphipod Eulimnogammarus verrucosus

Physiological responses to salinity stress in the Managua Cichlid,Cichlasoma managuense

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