The role of heat shock proteins 27 and 70 in redox-dependent regulation of apoptosis in Jurkat tumor cells

Носарева, О. Л.; Ryazantseva, N. V.; Stepovaya, E. A.; Шахристова, Е. В.; Stepanova, Ekaterina; Gulaya, V. S.

doi:10.18097/pbmc20166206670

Cited by 7 publications

(4 citation statements)

References 19 publications

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“…It is worth mentioning that HSPH1 , HSPA6 , HSP70.1 , LOC102178315 (heat shock 70 kDa protein 1B), and HSPB1 were also highly expressed during catagen. These genes belong to the heat shock protein family and are involved in stress resistance, such as ensuring the correct folding of proteins or controlling the targeting of proteins for subsequent degradation [ 49 – 51 ]. These proteins would therefore be useful in preventing apoptosis, making a response to DPs adjacent organization.…”

Section: Discussionmentioning

confidence: 99%

Integrative analysis reveals ncRNA-mediated molecular regulatory network driving secondary hair follicle regression in cashmere goats

Zhou

Kang

et al. 2018

BMC Genomics

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BackgroundCashmere is a keratinized product derived from the secondary hair follicles (SHFs) of cashmere goat skins. The cashmere fiber stops growing following the transition from the actively proliferating anagen stage to the apoptosis-driven catagen stage. However, little is known regarding the molecular mechanisms responsible for the occurrence of apoptosis in SHFs, especially as pertains to the role of non-coding RNAs (ncRNAs) and their interactions with other molecules. Hair follicle (HF) degeneration is caused by localized apoptosis in the skin, while anti-apoptosis pathways may coexist in adjacent HFs. Thus, elucidating the molecular interactions responsible for apoptosis and anti-apoptosis in the skin will provide insights into HF regression.ResultsWe used multiple-omics approaches to systematically identify long non-coding RNAs (lncRNAs), microRNAs (miRNAs) and mRNAs expressed in cashmere goat skins in two crucial phases (catagen vs. anagen) of HF growth. Skin samples were collected from three cashmere goats at the anagen (September) and catagen (February) stages, and six lncRNA libraries and six miRNA libraries were constructed for further analysis. We identified 1122 known and 403 novel lncRNAs in the goat skins, 173 of which were differentially expressed between the anagen and catagen stages. We further identified 3500 gene-encoding transcripts that were differentially expressed between these two phases. We also identified 411 known miRNAs and 307 novel miRNAs, including 72 differentially expressed miRNAs. We further investigated the target genes of lncRNAs via both cis- and trans-regulation during HF growth. Our data suggest that lncRNAs and miRNAs act synergistically in the HF growth transition, and the catagen inducer factors (TGFβ1 and BDNF) were regulated by miR-873 and lnc108635596 in the lncRNA-miRNA-mRNA networks.ConclusionThis study enriches the repertoire of ncRNAs in goats and other mammals, and contributes to a better understanding of the molecular mechanisms of ncRNAs involved in the regulation of HF growth and regression in goats and other hair-producing species.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4603-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Integrative analysis reveals ncRNA-mediated molecular regulatory network driving secondary hair follicle regression in cashmere goats

Zhou

Kang

et al. 2018

BMC Genomics

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“…It has been speculated that Hsp27 enhances the ability of migration and invasion of cancer cells by preventing dendritic cell maturation [ 6 ]. Blocking the expression of Hsp27 protein has become an important research direction in tumor targeted therapy [ 7 , 8 ]. The over-expression of Hsp27 has been detected in invasive tumor cells in breast carcinoma, oral squamous cell carcinoma, and pancreatic carcinoma [ 9 – 11 ].…”

Section: Discussionmentioning

confidence: 99%

Expression of Heat Shock Protein-27 (Hsp27) and P38MAPK in Esophageal Squamous Cell Carcinoma

et al. 2017

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BackgroundEsophageal squamous cell carcinoma (ESCC) is a worldwide concern. This study looked at the relationship between the expression of differential proteins and the clinicopathological data and survival rate of ESCC patients to identify potential tumor markers for the growth and metastasis of ESCC.Material/MethodsThis study included 162 patients who underwent surgical excision for management of ESCC. Fresh ESCC tissue and adjacent normal tissue specimens were collected. Protein expressions were detected by western blotting. The expression of Hsp27 and P38MAPK were detected by immunohistochemistry in formalin-fixed paraffin embedded primary tissue specimens.ResultsThe rate of positive Hsp27 and P38MAPK expression in ESCC tissue were higher than in normal esophageal tissue (p<0.05). The expression of P38MAPK was related to the depth of infiltration (p<0.05). The expression of Hsp27 was correlated with lymph node metastasis (p<0.05), but not with age, depth of infiltration, or tumor size. ROC were plotted to estimate the significance of the diagnosis: for Hsp27, AUC=0.735 (p<0.05), for P38MAPK, AUC=0.882 (p<0.05).ConclusionsThe expression of Hsp27 and P38MAPK plays a role in ESCC development. Hsp27 and P38MAPK could be used as prognostic factors in ESCC.

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“…Hsp27 is an ATP-dependent chaperone that folds substrate proteins and participates in shuttling destabilized or misfolded proteins toward proteolytic degradations [ 35 ]. Hsp27 is cytoprotective; it blocks apoptosis through interaction with various factors active in the apoptotic pathway [ 36 ]. The inhibition of apoptosis is required in the heat shock response (HSR) for cells to survive transient stress-induced apoptosis accompanied by the Hsp-mediated refolding of damaged proteins [ 37 ] ( Figure 2 ).…”

Section: Quantitative Levels and Functions Of Hsp27 Hsp60 Hsp70 And H...mentioning

confidence: 99%

The Chaperone System in Breast Cancer: Roles and Therapeutic Prospects of the Molecular Chaperones Hsp27, Hsp60, Hsp70, and Hsp90

Alberti

Vergilio

Paladino

et al. 2022

IJMS

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Breast cancer (BC) is a major public health problem, with key pieces of information needed for developing preventive and curative measures still missing. For example, the participation of the chaperone system (CS) in carcinogenesis and anti-cancer responses is poorly understood, although it can be predicted to be a crucial factor in these mechanisms. The chief components of the CS are the molecular chaperones, and here we discuss four of them, Hsp27, Hsp60, Hsp70, and Hsp90, focusing on their pro-carcinogenic roles in BC and potential for developing anti-BC therapies. These chaperones can be targets of negative chaperonotherapy, namely the elimination/blocking/inhibition of the chaperone(s) functioning in favor of BC, using, for instance, Hsp inhibitors. The chaperones can also be employed in immunotherapy against BC as adjuvants, together with BC antigens. Extracellular vesicles (EVs) in BC diagnosis and management are also briefly discussed, considering their potential as easily accessible carriers of biomarkers and as shippers of anti-cancer agents amenable to manipulation and controlled delivery. The data surveyed from many laboratories reveal that, to enhance the understanding of the role of the CS in BS pathogenesis, one must consider the CS as a physiological system, encompassing diverse members throughout the body and interacting with the ubiquitin–proteasome system, the chaperone-mediated autophagy machinery, and the immune system (IS). An integrated view of the CS, including its functional partners and considering its highly dynamic nature with EVs transporting CS components to reach all the cell compartments in which they are needed, opens as yet unexplored pathways leading to carcinogenesis that are amenable to interference by anti-cancer treatments centered on CS components, such as the molecular chaperones.

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The role of heat shock proteins 27 and 70 in redox-dependent regulation of apoptosis in Jurkat tumor cells

Cited by 7 publications

References 19 publications

Integrative analysis reveals ncRNA-mediated molecular regulatory network driving secondary hair follicle regression in cashmere goats

Integrative analysis reveals ncRNA-mediated molecular regulatory network driving secondary hair follicle regression in cashmere goats

Expression of Heat Shock Protein-27 (Hsp27) and P38MAPK in Esophageal Squamous Cell Carcinoma

The Chaperone System in Breast Cancer: Roles and Therapeutic Prospects of the Molecular Chaperones Hsp27, Hsp60, Hsp70, and Hsp90

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