The Role of Haemophilus ducreyi in Penile Ulcers in Bangkok, Thailand

Taylor, David N.; Duangmani, Chiraphun; Suvongse, Chaninthorn; O’Connor, Robert J.; Pitarangsi, Chittima; Panikabutra, Kanchana; Echeverria, Peter

doi:10.1097/00007435-198407000-00005

Cited by 51 publications

(23 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other etiologic agents of genital ulcers such as herpes simplex virus (36,37,83,163) and Treponema pallidum (36,37,103,122,156) One of the problems in determining the true incidence of chancroid is that the diagnosis has often been based on the clinical appearance of the lesion and the exclusion of other pathogens. Less than 12% of the 5,409 cases of chancroid reported in the nine U.S. outbreaks since 1981 were confirmed by culture.…”

Section: Epidemiologymentioning

confidence: 99%

Chancroid and Haemophilus ducreyi

1989

View full text Add to dashboard Cite

Haemophilus ducreyi is the causative agent of chancroid, one of the genital ulcerative diseases. H. ducreyi is the major cause of genital ulcer disease in Africa and Southeast Asia and is of increasing concern in the United States. Definitive diagnosis of chancroid requires the isolation and identification of H. ducreyi, but isolation of this organism is difficult and the available medium is not optimal for all strains. Fluorescent antibody and serologic tests are of limited value. In general, our knowledge of this organism is rather limited, and indeed, recent studies have questioned the placement of H. ducreyi in the genus Haemophilus. H. ducreyi has relatively few biochemical activities, and epidemiologic studies are limited because there are limited phenotypic markers available for strain typing. Specific virulence factors of H. ducreyi have yet to be identified. Antimicrobial resistance in H. ducreyi is of special concern, as this organism has acquired both gram-negative and gram-positive resistance determinants. In addition, some of these determinants can be mobilized and transferred to other Haemophilus species or to Neisseria gonorrhoeae.

show abstract

Section: Epidemiologymentioning

confidence: 99%

Chancroid and Haemophilus ducreyi

1989

View full text Add to dashboard Cite

show abstract

“…13-l Using this medium they cultured an organism said to be H ducreyi from over a quarter of patients with genital ulcers as well as from several people without symptoms presenting to the local clinic for sexually transmitted diseases. 4 16 These studies are contrary to those from other Western cities, including Liverpool,'7 Manchester,'8 Winnipeg,'9 and Antwerp,20 where H ducreyi was isolated only rarely with conventional media and always from people with genital ulcers, which were usually acquired abroad. The Sheffield medium has been tested in the tropics, and its lack of sensitivity suggests that it is detecting organisms other than H ducreyi.…”

Section: Chancroidmentioning

confidence: 89%

Chancroid.

Mindel¹

1989

BMJ

View full text Add to dashboard Cite

“…ducreyi, in practice this characteristic appearance is rarely demonstrated in ulcer exudate (Nzanze et al, 1981). Some authorities have found the evidence of gram-stained smears to be specific and sensitive (Taylor et al, 1984), but others have not (Chapel et al, 1977;Nsanze et al, 1981;Choudhary et al, 1982).…”

Section: Discussionmentioning

confidence: 99%

“…ducreyi has few demonstrable biochemical characteristics (Ronald and Albritton 1984) because of its fastidious growth requirements. Reports on the key identification tests of this organism grown on different media are conflicting (Sottnek et al, 1980;Sturm and Zanen, 1984;Taylor al., 1984). Furthermore, there is Speculation that different biotypes of this organism may exist in different parts of the world (Sturm and Zanen, 1984).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Haemophilus Ducreyi in a Clinical Laboratory

Lubwama¹,

Plummer

Ndinya-Achola

et al. 1986

Journal of Medical Microbiology

View full text Add to dashboard Cite

Summary. Routine procedures used to isolate Haemophilus ducreyi in a busy laboratory are reported. Identification was based on colony morphology and nutritional and biochemical properties of 120 fresh isolates of H. ducreyi. These isolates grew very well on Gonococcal Agar and Mueller-Hinton Agar incubated at 34°C in candle extinction jars containing moistened filter paper. Colonies varied in size, giving a polymorphic appearance. They were smooth, dome-shaped, and buffyellow to grey in colour, and measured 2 mm in diameter. They could be pushed intact across the agar surface. By microscopic examination of gram-stained smears the isolates were gram-negative coccobacilli arranged in short chains, clumps or whorls and occasionally in typical "rail track" arrangements. Individual bacteria showed bipolar staining. Colonies autoagglutinated in saline. All strains were catalasenegative and did not produce indole or H2S. They were oxidase-and p-lactamase positive and required X but not V factor for growth. Now that reliable techniques have been developed and characteristics established it is possible for most clinical laboratories to isolate and identify this organism from most patients with chancroid.

show abstract

The Role of Haemophilus ducreyi in Penile Ulcers in Bangkok, Thailand

Cited by 51 publications

References 11 publications

Chancroid and Haemophilus ducreyi

Chancroid and Haemophilus ducreyi

Chancroid.

Isolation and Identification of Haemophilus Ducreyi in a Clinical Laboratory

Contact Info

Product

Resources

About