The Role of H2O2-Scavenging Enzymes (Ascorbate Peroxidase and Catalase) in the Tolerance of Lemna minor to Antibiotics: Implications for Phytoremediation

Abstract

We investigated the individual and combined contributions of two distinct heme proteins namely, ascorbate peroxidase (APX) and catalase (CAT) on the tolerance of Lemna minor plants to antibiotics. For our investigation, we used specific inhibitors of these two H2O2-scavenging enzymes (p-aminophenol, 3-amino,1,2,4-triazole, and salicylic acid). APX activity was central for the tolerance of this aquatic plant to amoxicillin (AMX), whereas CAT activity was important for avoiding oxidative damage when exposed to ciprofloxacin (CIP). Both monitored enzymes had important roles in the tolerance of Lemna minor to erythromycin (ERY). The use of molecular kinetic approaches to detect and increase APX and/or CAT scavenging activities could enhance tolerance, and, therefore, improve the use of L. minor plants to reclaim antibiotics from water bodies.

Antioxidant Enzyme and Cytochrome P450 activities are involved in horseweed (<em>Conyza sumatrensis</em>) Resistance to Glyphosate

“…APX and CAT are important antioxidant enzymes involved in H2O2 scavenging, and therefore in the avoidance of oxidative bursts caused by the accumulation of reactive oxygen species. Additionally, the increased activities of those enzymes have been reported in cases of plant tolerance to xenobiotics 42 , including glyphosate 43 . In contrast to H2O2 scavenging enzymes (APX and CAT), SOD appears not to be involved in glyphosate resistance, as enzyme activity differed between the glyphosate exposed biotypes only at 24 h (Fig.…”

Investigating the role of bentonite clay with different soil amendments to minimize the bioaccumulation of heavy metals in Solanum melongena L. under the irrigation of tannery wastewater

“…Studies have shown that Cr-contaminated soil causes oxidative stress in plants, disrupting the oxidants and antioxidants balance of plants ( Espina et al, 2014 ). APX and CAT are responsible for regulating the photosynthetic mechanism of the plant under adverse environmental conditions ( Zaheer et al, 2019a , b ; Gomes et al, 2022 ). Activities of APX and CAT enzymes in plants perform important H 2 O 2 scavenging activities through more production of hydroxyl groups ( Ahmad et al, 2020 ; Gomes et al, 2022 ).…”

“…APX and CAT are responsible for regulating the photosynthetic mechanism of the plant under adverse environmental conditions ( Zaheer et al, 2019a , b ; Gomes et al, 2022 ). Activities of APX and CAT enzymes in plants perform important H 2 O 2 scavenging activities through more production of hydroxyl groups ( Ahmad et al, 2020 ; Gomes et al, 2022 ). Results revealed that APX and CAT, both antioxidant enzymes, worked synergistically to activate plants’ defensive mechanism against ROS through FOP + BT and CFOP applications most competitively under Cr and Pb stress ( Figure 6 ).…”

Discovering Diverse Roles of Peroxidases and Catalases in Photosynthetic and Non-Photosynthetic Eukaryotes

“…Both enzymatic activities here investigated, namely, peroxidase with ascorbate as electron donor and catalase, were subjected to detailed analyses in Lemna minor plants within the acquired tolerance of this aquatic freshwater plant to antibiotics amoxicillin, ciprofloxacin, and erythromycin, which pose a global contamination problem in various environments [ 2 ]. The resulting effects, tested with specific inhibitors, were different for peroxidase and catalase activities.…”