The role of glutathione in yeast dehydration tolerance

Espíndola, A; Gomes, Débora Silva; Panek, Anita D.; Eleuthério, Elis C. A.

doi:10.1016/j.cryobiol.2003.10.003

Cited by 56 publications

(34 citation statements)

References 22 publications

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“…Therefore, such strains are excluded from the commercial catalogues of yeast manufacturers, awaiting the breakthrough that would allow their optimization. Most previous physiological studies on yeast dehydration tolerance have been performed on intracellular glutathione concentration (de Souza Espindola et al 2003); the role of cytoplasmic catalase (França et al 2005); cell viability at different drying kinetics (Beney et al 2000); drying in the presence of chemic-protecting drugs (Beker and Rapoport 1987); osmotic pressure during the cell-dehydration and -rehydration process (Simonin et al 2007); the effect of magnesium complementation during the yeast rehydration process (Rodríguez-Porrata et al 2008); and when nitrogen catabolite repression is active during the rehydration phase (Vaudano et al 2009). In apparent contradiction with the prevailing intracellular trehalose-based model for S. cerevisiae to tolerate desiccation, Ratnakumar and Tunnacliffe (2006) demonstrated that desiccation tolerance in a mutant strain unrelated to the production of trehalose (deleted trehalose-6-phosphate synthase gene, tps1D), and also that an increasing degree of tolerance exists after diauxic shift or heat stress, albeit slightly less than in the wild type.…”

Section: Introductionmentioning

confidence: 99%

Enhancing yeast cell viability after dehydration by modification of the lipid profile

Rodríguez-Porrata

López-Martínez

Redón

et al. 2010

World J Microbiol Biotechnol

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In the present study, we analysed metabolite features during the dehydration-rehydration process for different yeast species genetically closely related to S. cerevisiae, in order to determine whether metabolites might play a role in cell viability. We ranked the species S. cerevisiae, S. paradoxus, S. kudriavzevii, L. kluyveri, N. castellii, S. mikatae, S. bayanus, and S. servazzii according to their viability rate after the dehydrationrehydration process, and showed that desiccation tolerance across the species did not correlate with the intracellular content of trehalose or glycogen. Cell lipid composition was also investigated during this process, to see whether the content of triacylglycerols and phosphatidylcholine showed significant variations across the species. The increase of phosphatidylcholine level increase in both S. paradoxus and S. bayanus cells grown in supplemented media enhanced both their cell viability after stress imposition and lipid storage.

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Section: Introductionmentioning

confidence: 99%

Enhancing yeast cell viability after dehydration by modification of the lipid profile

Rodríguez-Porrata

López-Martínez

Redón

et al. 2010

World J Microbiol Biotechnol

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“…Quantification of lipid peroxidation was carried out by the reaction of thiobarbituric acid with the malondialdehyde product of oxidized fatty acid breakage (18). Cells were collected and then extracted by vortexing with 1 volume of glass beads in 0.5 ml of 50 mM sodium phosphate buffer (pH 6.0)-10% trichloroacetic acid with FastPrep 24.…”

Section: Methodsmentioning

confidence: 99%

Oxidative Stress Tolerance, Adenylate Cyclase, and Autophagy Are Key Players in the Chronological Life Span of Saccharomyces cerevisiae during Winemaking

Orozco

Matallana

Aranda

2012

Appl Environ Microbiol

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bMost grape juice fermentation takes place when yeast cells are in a nondividing state called the stationary phase. Under such circumstances, we aimed to identify the genetic determinants controlling longevity, known as the chronological life span. We identified commercial strains with both short (EC1118) and long (CSM) life spans in laboratory growth medium and compared them under diverse conditions. Strain CSM shows better tolerance to stresses, including oxidative stress, in the stationary phase. This is reflected during winemaking, when this strain has an increased maximum life span. Compared to EC1118, CSM overexpresses a mitochondrial rhodanese gene-like gene, RDL2, whose deletion leads to increased reactive oxygen species production at the end of fermentation and a correlative loss of viability at this point. EC1118 shows faster growth and higher expression of glycolytic genes, and this is related to greater PKA activity due to the upregulation of the adenylate cyclase gene. This phenotype has been linked to the presence of a ␦ element in its promoter, whose removal increases the life span. Finally, EC1118 exhibits a higher level of protein degradation by autophagy, which might help achieve fast growth at the expense of cellular structures and may be relevant for long-term survival under winemaking conditions.

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“…Consistent with these findings, Z3-86 showed 38.2% and 27.5% more GSH than ZTW1 under both normal and osmotic conditions than ZTW1 (Table 5; t test, P < 0.05). Although enzymatic antioxidant systems are effective against ROS under conditions of sufficient water, only molecular antioxidants such as GSH and trehalose can alleviate oxidative stress when water is insufficient [40]. These molecular antioxidants immobilize the cytoplasm to a glassy state, thereby preventing chemical reactions, molecular diffusion, and conformational changes in biomolecules and protecting cell membranes under water deficiency [41].…”

Section: Resultsmentioning

confidence: 99%

Construction of Novel Saccharomyces cerevisiae Strains for Bioethanol Active Dry Yeast (ADY) Production

et al. 2013

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The application of active dry yeast (ADY) in bioethanol production simplifies operation processes and reduces the risk of bacterial contamination. In the present study, we constructed a novel ADY strain with improved stress tolerance and ethanol fermentation performances under stressful conditions. The industrial Saccharomyces cerevisiae strain ZTW1 showed excellent properties and thus subjected to a modified whole-genome shuffling (WGS) process to improve its ethanol titer, proliferation capability, and multiple stress tolerance for ADY production. The best-performing mutant, Z3-86, was obtained after three rounds of WGS, producing 4.4% more ethanol and retaining 2.15-fold higher viability than ZTW1 after drying. Proteomics and physiological analyses indicated that the altered expression patterns of genes involved in protein metabolism, plasma membrane composition, trehalose metabolism, and oxidative responses contribute to the trait improvement of Z3-86. This work not only successfully developed a novel S. cerevisiae mutant for application in commercial bioethanol production, but also enriched the current understanding of how WGS improves the complex traits of microbes.

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The role of glutathione in yeast dehydration tolerance

Cited by 56 publications

References 22 publications

Enhancing yeast cell viability after dehydration by modification of the lipid profile

Enhancing yeast cell viability after dehydration by modification of the lipid profile

Oxidative Stress Tolerance, Adenylate Cyclase, and Autophagy Are Key Players in the Chronological Life Span of Saccharomyces cerevisiae during Winemaking

Construction of Novel Saccharomyces cerevisiae Strains for Bioethanol Active Dry Yeast (ADY) Production

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