“…Since CO-mediated inhibition of mitochondrial oxidative metabolism can increase the amount of mitochondrial (mt) ROS, a typical phenotype observed with an impaired ETC in cancer cells [23], we measured ROS in mitochondrial extracts and thiobarbituric reactive substances (TBARS), i.e., lipoperoxidation products that indicate the presence of oxidative stress. Compound 1 increased both mtROS and mtTBARS, whereas cisplatin, doxorubicin, gemcitabine, and docetaxel are devoid of effects, although they have been reported to induce oxidative stress in sensitive cells [31][32][33][34] (Figure 6A,B, Supplementary Figure S3A,B). A similar increase in ROS and lipoperoxidation was observed in whole cell lysates (Figure 6C,D, Supplementary Figure S3C,D).…”