The Role of Genomics and Biotechnology in Achieving Global Food Security for High-Oleic Vegetable Oil

Wilson, Richard F.

doi:10.5650/jos.61.357

Cited by 48 publications

(30 citation statements)

References 34 publications

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“…Consumer awareness of oil quality is becoming an increasingly important variable that conditions shifts in the food ingredient selection process, thereby creating new market opportunities (Wilson 2012). Acceleration of breeding cycles could translate into the edge necessary to respond to these new market demands in a timely fashion.…”

Section: Introductionmentioning

confidence: 99%

Association mapping of seed quality traits using the Canadian flax (Linum usitatissimum L.) core collection

et al. 2014

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Key messageThe identification of stable QTL for seed quality traits by association mapping of a diverse panel of linseed accessions establishes the foundation for assisted breeding and future fine mapping in linseed.AbstractLinseed oil is valued for its food and non-food applications. Modifying its oil content and fatty acid (FA) profiles to meet market needs in a timely manner requires clear understanding of their quantitative trait loci (QTL) architectures, which have received little attention to date. Association mapping is an efficient approach to identify QTL in germplasm collections. In this study, we explored the quantitative nature of seed quality traits including oil content (OIL), palmitic acid, stearic acid, oleic acid, linoleic acid (LIO) linolenic acid (LIN) and iodine value in a flax core collection of 390 accessions assayed with 460 microsatellite markers. The core collection was grown in a modified augmented design at two locations over 3 years and phenotypic data for all seven traits were obtained from all six environments. Significant phenotypic diversity and moderate to high heritability for each trait (0.73–0.99) were observed. Most of the candidate QTL were stable as revealed by multivariate analyses. Nine candidate QTL were identified, varying from one for OIL to three for LIO and LIN. Candidate QTL for LIO and LIN co-localized with QTL previously identified in bi-parental populations and some mapped nearby genes known to be involved in the FA biosynthesis pathway. Fifty-eight percent of the QTL alleles were absent (private) in the Canadian cultivars suggesting that the core collection possesses QTL alleles potentially useful to improve seed quality traits. The candidate QTL identified herein will establish the foundation for future marker-assisted breeding in linseed.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-014-2264-4) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Association mapping of seed quality traits using the Canadian flax (Linum usitatissimum L.) core collection

et al. 2014

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“…Recently, Plenish high oleic soybeans have been in commercial production by DuPont since 2012. They were developed using a biotech process known as gene silencing [22]. These results demonstrate that the GmFAD2 family members play a very important role in metabolically engineered oil seed plants [23].…”

Section: Introductionmentioning

confidence: 99%

Changes in Oleic Acid Content of Transgenic Soybeans by Antisense RNA Mediated Posttranscriptional Gene Silencing

Zhang

Yang

Zhang

et al. 2014

International Journal of Genomics

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The Delta-12 oleate desaturase gene (FAD2-1), which converts oleic acid into linoleic acid, is the key enzyme determining the fatty acid composition of seed oil. In this study, we inhibited the expression of endogenous Delta-12 oleate desaturase GmFad2-1b gene by using antisense RNA in soybean Williams 82. By employing the soybean cotyledonary-node method, a part of the cDNA of soybean GmFad2-1b 801 bp was cloned for the construction of a pCAMBIA3300 vector under the soybean seed promoter BCSP. Leaf painting, LibertyLink strip, PCR, Southern blot, qRT-PCR, and fatty acid analysis were used to detect the insertion and expression of GmFad2-1b in the transgenic soybean lines. The results indicate that the metabolically engineered plants exhibited a significant increase in oleic acid (up to 51.71%) and a reduction in palmitic acid (to <3%) in their seed oil content. No structural differences were observed between the fatty acids of the transgenic and the nontransgenic oil extracts.

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“…The data presented here also show an increase in the monounsaturated fatty acid oleic acid (18:1) in bioengineered maize seeds. Also, partial hydrogenation of polyunsaturated oils is used to produce high-oleic acid corn oil, a technology with potential of capturing >40% of the domestic consumption of vegetable oil in the United States by 2020 due to its health benefits (Wilson 2012). However, as per Aranceta and Pérez-Rodrigo (2012) and Dyson et al (2011), foods rich in monounsaturated fatty acids is considered important to improve glucose-insulin profiles and reduce the effects of Type 2 diabetes.…”

Section: Discussionmentioning

confidence: 99%

Metabolic Engineering to Increase the Corn Seed Storage Lipid Quantity and Change Its Compositional Quality

et al. 2017

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Given limited global food supplies and the fact that the global population is expected to double by 2050, there is an urgent need for the development of high‐calorie foods, including culinary oils. The seeds of oil crops contain high‐energy density oil composed of triacylglycerides (TAGs) at up to 80% by dry mass. However, maize (Zea mays L.) seeds are relatively poor in calorie and nutritional values. Therefore, in this report, we address this constraint via metabolic engineering to improve maize seed lipids including TAG and seed TAG nutritional values by overexpression of three major genes, including: (i) the Arabidopsis thaliana (L.) Heynh. diacylglycerol acyltransferase 1 (AtDGAT1), a gene that catalyzes the TAG biosynthesis final step prior to packaging of TAGs into oil bodies; (ii) the transcription factor WRINKLED 1 (WRI1), which promotes the regulation of the expression of genes involved in fatty acid biosynthesis; and (i) the A. thaliana oleosin (AtOleosin) gene, a gene coding for a protein that protects TAGs from degradation. The overexpression of the above three genes, and probably certain unintentional in vitro culture genetic variabilities, resulted in 117% increase of seed TAGs and 25% increase in total oil contents when compared with the wild‐type control corn seeds. In addition, the above genetic modifications led to major shifts in the fatty acid profiles in favor of human health.

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The Role of Genomics and Biotechnology in Achieving Global Food Security for High-Oleic Vegetable Oil

Cited by 48 publications

References 34 publications

Association mapping of seed quality traits using the Canadian flax (Linum usitatissimum L.) core collection

Association mapping of seed quality traits using the Canadian flax (Linum usitatissimum L.) core collection

Changes in Oleic Acid Content of Transgenic Soybeans by Antisense RNA Mediated Posttranscriptional Gene Silencing

Metabolic Engineering to Increase the Corn Seed Storage Lipid Quantity and Change Its Compositional Quality

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