The role of G-proteins in plant immunity

Zhang, Huajian; Gao, Zhimou; Zheng, Xiaobo; Zhang, Zhengguang

doi:10.4161/psb.21431

Cited by 45 publications

(25 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…G-protein signaling pathways have been extensively studied in animals because of their role in regulating almost every physiological response including vision, olfaction, neurotransmission and hormone signaling (Cabrera-Vera et al, 2003;Offermanns, 2003;McCudden et al, 2005;. Recent studies suggest similar multifaceted roles for G-proteins during plant growth and development; however, relatively few molecular details of their mechanisms have been elucidated (Zhang et al, 2012;Urano et al, 2013;Urano & Jones, 2014). G-proteins function as molecular switches.…”

Section: Introductionmentioning

confidence: 99%

Gα and regulator of G‐protein signaling (RGS) protein pairs maintain functional compatibility and conserved interaction interfaces throughout evolution despite frequent loss of RGS proteins in plants

et al. 2016

View full text Add to dashboard Cite

Signaling pathways regulated by heterotrimeric G-proteins exist in all eukaryotes. The regulator of G-protein signaling (RGS) proteins are key interactors and critical modulators of the Gα protein of the heterotrimer. However, while G-proteins are widespread in plants, RGS proteins have been reported to be missing from the entire monocot lineage, with two exceptions. A single amino acid substitution-based adaptive coevolution of the Gα:RGS proteins was proposed to enable the loss of RGS in monocots. We used a combination of evolutionary and biochemical analyses and homology modeling of the Gα and RGS proteins to address their expansion and its potential effects on the G-protein cycle in plants. Our results show that RGS proteins are widely distributed in the monocot lineage, despite their frequent loss. There is no support for the adaptive coevolution of the Gα:RGS protein pair based on single amino acid substitutions. RGS proteins interact with, and affect the activity of, Gα proteins from species with or without endogenous RGS. This cross-functional compatibility expands between the metazoan and plant kingdoms, illustrating striking conservation of their interaction interface. We propose that additional proteins or alternative mechanisms may exist which compensate for the loss of RGS in certain plant species.

show abstract

Section: Introductionmentioning

confidence: 99%

Gα and regulator of G‐protein signaling (RGS) protein pairs maintain functional compatibility and conserved interaction interfaces throughout evolution despite frequent loss of RGS proteins in plants

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Plant G proteins play an important regulatory role in multiple physiological processes. They are involved in signal transduction from hormone receptors, including the plant hormones gibberellin and abscisic acid that regulate gene expression; secretion, defence, stomata movements, channel regulation, sugar sensing and cell death [ 44 ]. The heterotrimeric G protein complex is minimally composed of three subunits: alpha, beta and gamma.…”

Section: Resultsmentioning

confidence: 99%

Identification of Nicotiana benthamiana microRNAs and their targets using high throughput sequencing and degradome analysis

et al. 2015

View full text Add to dashboard Cite

BackgroundNicotiana benthamiana is a widely used model plant species for research on plant-pathogen interactions as well as other areas of plant science. It can be easily transformed or agroinfiltrated, therefore it is commonly used in studies requiring protein localization, interaction, or plant-based systems for protein expression and purification. To discover and characterize the miRNAs and their cleaved target mRNAs in N. benthamiana, we sequenced small RNA transcriptomes and degradomes of two N. benthamiana accessions and validated them by Northern blots.ResultsWe used a comprehensive molecular approach to detect and to experimentally validate N. benthamiana miRNAs and their target mRNAs from various tissues. We identified 40 conserved miRNA families and 18 novel microRNA candidates and validated their target mRNAs with a genomic scale approach. The accumulation of thirteen novel miRNAs was confirmed by Northern blot analysis. The conserved and novel miRNA targets were found to be involved in various biological processes including transcription, RNA binding, DNA modification, signal transduction, stress response and metabolic process. Among the novel miRNA targets we found the mRNA of REPRESSOR OF SILENCING (ROS1). Regulation of ROS1 by a miRNA provides a new regulatory layer to reinforce transcriptional gene silencing by a post-transcriptional repression of ROS1 activity.ConclusionsThe identified conserved and novel miRNAs along with their target mRNAs also provides a tissue specific atlas of known and new miRNA expression and their cleaved target mRNAs of N. benthamiana. Thus this study will serve as a valuable resource to the plant research community that will be beneficial well into the future.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-2209-6) contains supplementary material, which is available to authorized users.

show abstract

“…tomato containing avrRpm1 and RPM1 in Arabidopsis 23 , 24 . Heterotrimeric G-proteins, well known in stress signaling 25 , have been proposed as activators of plant cell death and mediators of stomatal closure signaling 26 as well as are involved in cell wall biogenesis/metabolism and ABA signaling 27 , 28 . The induction of G-protein signaling in DVI suggested its function in early defense response against Foc.…”

Section: Discussionmentioning

confidence: 99%

Chickpea-Fusarium oxysporum interaction transcriptome reveals differential modulation of plant defense strategies

Upasani

Limaye

Gurjar

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Fusarium wilt is one of the major biotic stresses reducing chickpea productivity. The use of wilt-resistant cultivars is the most appropriate means to combat the disease and secure productivity. As a step towards understanding the molecular basis of wilt resistance in chickpea, we investigated the transcriptomes of wilt-susceptible and wilt-resistant cultivars under both Fusarium oxysporum f.sp. ciceri (Foc) challenged and unchallenged conditions. Transcriptome profiling using LongSAGE provided a valuable insight into the molecular interactions between chickpea and Foc, which revealed several known as well as novel genes with differential or unique expression patterns in chickpea contributing to lignification, hormonal homeostasis, plant defense signaling, ROS homeostasis, R-gene mediated defense, etc. Similarly, several Foc genes characteristically required for survival and growth of the pathogen were expressed only in the susceptible cultivar with null expression of most of these genes in the resistant cultivar. This study provides a rich resource for functional characterization of the genes involved in resistance mechanism and their use in breeding for sustainable wilt-resistance. Additionally, it provides pathogen targets facilitating the development of novel control strategies.

show abstract

The role of G-proteins in plant immunity

Cited by 45 publications

References 64 publications

Gα and regulator of G‐protein signaling (RGS) protein pairs maintain functional compatibility and conserved interaction interfaces throughout evolution despite frequent loss of RGS proteins in plants

Gα and regulator of G‐protein signaling (RGS) protein pairs maintain functional compatibility and conserved interaction interfaces throughout evolution despite frequent loss of RGS proteins in plants

Identification of Nicotiana benthamiana microRNAs and their targets using high throughput sequencing and degradome analysis

Chickpea-Fusarium oxysporum interaction transcriptome reveals differential modulation of plant defense strategies

Contact Info

Product

Resources

About