The Role of Fusobacterium nucleatum in Colorectal Cancer Cell Proliferation and Migration

Wu, Zihong; Ma, Qiang; Guo, Ying; You, Fengming

doi:10.3390/cancers14215350

Cited by 10 publications

(5 citation statements)

References 87 publications

(128 reference statements)

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“…This cascade, in turn, engenders tumor cell proliferation and migration. 28 , 76 Furthermore, Gram-negative bacteria, including F. nucleatum , release outer membrane vesicles (OMVs), extracellular vesicles containing cytoplasmic and periplasmic components enclosed within a protein-lipid bilayer. 77 F. nucleatum has been substantiated as an activator of TLR4 through the discharge of OMVs, instigating a sequence that involves NF-κB, CREB, extracellular signal-regulated kinases, and inflammatory cytokines within human cell lines and colon-like monolayers.…”

Section: F Nucleatum and Tumorigenesis Tumor Metastasismentioning

confidence: 99%

Fusobacterium nucleatum in tumors: from tumorigenesis to tumor metastasis and tumor resistance

Ye,

Liu,

Liu

et al. 2024

Cancer Biology & Therapy

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Fusobacterium nucleatum , an anaerobic Gram-negative bacterium primarily residing in the oral cavity, has garnered significant attention for its emerging role in cancer progression and prognosis. While extensive research has revealed mechanistic links between Fusobacterium nucleatum and colorectal cancer, a comprehensive review spanning its presence and metastatic implications in cancers beyond colorectal origin is conspicuously absent. This paper broadens our perspective from colorectal cancer to various malignancies associated with Fusobacterium nucleatum , including oral, pancreatic, esophageal, breast, and gastric cancers. Our central focus is to unravel the mechanisms governing Fusobacterium nucleatum colonization, initiation, and promotion of metastasis across diverse cancer types. Additionally, we explore Fusobacterium nucleatum ‘s adverse impacts on cancer therapies, particularly within the domains of immunotherapy and chemotherapy. Furthermore, this paper underscores the clinical research significance of Fusobacterium nucleatum as a potential tumor biomarker and therapeutic target, offering a novel outlook on its applicability in cancer detection and prognostic assessment.

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Section: F Nucleatum and Tumorigenesis Tumor Metastasismentioning

confidence: 99%

Fusobacterium nucleatum in tumors: from tumorigenesis to tumor metastasis and tumor resistance

Ye,

Liu,

Liu

et al. 2024

Cancer Biology & Therapy

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“…Notably, an increased presence of Fusobacterium nucleatum in CRC patients compared to healthy individuals has emerged as a significant marker for CRC diagnosis (5). Studies have shown that F. nucleatum promotes CRC progression by activating signaling pathways such as Ecadherin/β-catenin and TLR4/MyD88, and modulating autophagy and immune responses (6). Moreover, F. nucleatum is implicated in various molecular events, including microsatellite instability (MSI), CpG island methylator phenotype (CIMP), and tumorigenic mutations in genes such as TP53, BRAF, CHD7, and CHD8, Jundishapur J Microbiol.…”

Section: Introductionmentioning

confidence: 99%

Associations Between Fusobacterium nucleatum and msh2, mlh1, and msh6 Gene Expression in Colorectal Cancer

Torkashvand,

Hajikhani,

Hosseini Doust

et al. 2024

Jundishapur J Microbiol

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Background: Colorectal cancer (CRC) is a major global health concern, and the link with Fusobacterium nucleatum has received considerable attention. Objectives: This study aimed to explore the prevalence of F. nucleatum and to assess the expression of the msh2, mlh1, and msh6 genes in CRC patients compared to a control group using real-time PCR. Methods: Forty CRC patients and twenty individuals from a control group participated in this study. Gastroenterologists collected biopsy specimens from which DNA and RNA were extracted using a specialized tissue extraction kit. Complementary DNA (cDNA) was then synthesized. Real-time PCR was employed to evaluate the expression levels of the msh2, mlh1, and msh6 genes and the presence of the F. nucleatum-specific 16srRNA gene to determine the relative prevalence of this bacterium in each group. Results: Results indicated a higher prevalence of the F. nucleatum-specific 16srRNA gene in CRC patients than in the control group. Additionally, expression levels of the msh2, mlh1, and msh6 genes were significantly higher in the cancer group, suggesting their role in CRC pathogenesis. The distribution of F. nucleatum was particularly high in the sigmoid and rectum areas of the colon. Conclusions: This study underscores the significance of F. nucleatum in CRC and provides insights into its association with altered gene expression patterns. Understanding the prevalence of F. nucleatum and its impact on msh2, mlh1, and msh6 genes may aid in developing improved diagnostic and therapeutic strategies for CRC. Further research is necessary to elucidate these relationships more comprehensively.

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“…Various studies have shown an increase in Fusobacterium species in patients with CRC compared with healthy individuals and inflammatory tissue of the colon in the precancerous phase [ 11 , 12 ]. Fusobacterium nucleatum ( F. nucleatum ) is an anaerobic gram-negative bacterium that uses its pathogenic factors, such as adhesion molecules and lipopolysaccharide, to induce inflammation and activate the immune system's immune responses through various cellular signaling pathways [ 13 , 14 ]. According to the results of studies on the early detection and diagnosis of CRC, various genetic factors can be used as molecular markers for the early detection of CRC, including the family of miRNAs [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Association between colorectal cancer and expression levels of miR-21, miR-17-5P, miR-155 genes and the presence of Fusobacterium nucleatum in biopsy samples obtained from Iranian patients

Bostanshirin

Hajikhani

Vaezi

et al. 2023

Infect Agents Cancer

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Background Colorectal cancer (CRC) is considered the second-deadliest and third-most common malignancy worldwide. Studying the carcinogenic mechanism of bacteria or their role in aggravating cancer can be precious. Fusobacterium nucleatum (F. nucleatum) is one of the important bacteria in the occurrence and spread of CRC. In this study, we investigated the expression levels of miR-21, miR-17-5P, miR-155, and the relative frequency of F. nucleatum in biopsy samples from patients with CRC. Method DNA and RNA samples were extracted using a tissue extraction kit, and then cDNAs were synthesized using a related kit. Based on the sequence of miR-17-5P, miR-21, and miR-155 genes, F. nucleatum specific 16srRNA and bacterial universal16srRNA specific primers were selected, and the expression levels of the target genes were analyzed using the Real-Time PCR method. Results The expression level of miR-21, miR-17-5P, and miR-155 genes showed a significant increase in the cancer group. Also, the expression of the mentioned miRNAs was significantly raised in the positive samples for F. nucleatum presence. The relative frequency of F. nucleatum in the cancer group was significantly increased compared to the control group. Conclusion Due to the changes in the expression of genes involved in causing CRC in the presence of F. nucleatum, it is possible to prompt identification and provide therapeutic solutions to cancer patients by studying their microbial profiles and the expression changes of different selected genes.

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The Role of Fusobacterium nucleatum in Colorectal Cancer Cell Proliferation and Migration

Cited by 10 publications

References 87 publications

Fusobacterium nucleatum in tumors: from tumorigenesis to tumor metastasis and tumor resistance

Fusobacterium nucleatum in tumors: from tumorigenesis to tumor metastasis and tumor resistance

Associations Between Fusobacterium nucleatum and msh2, mlh1, and msh6 Gene Expression in Colorectal Cancer

Association between colorectal cancer and expression levels of miR-21, miR-17-5P, miR-155 genes and the presence of Fusobacterium nucleatum in biopsy samples obtained from Iranian patients

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