The role of focal adhesion kinase in transforming growth factor-β2 induced migration of human lens epithelial cells

Liu, Jie; Xu, Dan; Li, Jingming; Gao, Ning; Liao, Chongbing; Jing, Ruihua; Wu, Bogang; Ma, Bo; Shao, Yongping; Pei, Cheng

doi:10.3892/ijmm.2018.3912

Cited by 4 publications

(5 citation statements)

References 49 publications

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“…Various genes are involved in the migration of LECs [ 19 , 21 , 33 , 34 , 35 ]. We observed an increased expression of MMP9 at 1 and 2 Gy ( Figure 6 A).…”

Section: Discussionmentioning

confidence: 99%

“…An increase in MMP9 protein levels has previously been identified following proton irradiation [ 10 ]. Liu et al [ 35 ] showed that an increase in TGFβ2 expression significantly increased migration and that inhibition of PTK2 decreased TGFβ2-mediated cell migration, indicating that both genes have a role in the migration of LECs. In the present study, no significant changes were observed in the expression of PTK2 ( Figure 6 B), suggesting that this gene does not play a role in the changes in migration following low doses of radiation.…”

Section: Discussionmentioning

confidence: 99%

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Radiation-Induced Alterations in Proliferation, Migration, and Adhesion in Lens Epithelial Cells and Implications for Cataract Development

et al. 2022

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The lens of the eye is one of the most radiosensitive tissues. Although the exact mechanism of radiation-induced cataract development remains unknown, altered proliferation, migration, and adhesion have been proposed as factors. Lens epithelial cells were exposed to X-rays (0.1–2 Gy) and radiation effects were examined after 12 h and 7 day. Proliferation was quantified using an MTT assay, migration was measured using a Boyden chamber and wound-healing assay, and adhesion was assessed on three extracellular matrices. Transcriptional changes were also examined using RT-qPCR for a panel of genes related to these processes. In general, a nonlinear radiation response was observed, with the greatest effects occurring at a dose of 0.25 Gy. At this dose, a reduction in proliferation occurred 12 h post irradiation (82.06 ± 2.66%), followed by an increase at 7 day (116.16 ± 3.64%). Cell migration was increased at 0.25 Gy, with rates 121.66 ± 6.49% and 232.78 ± 22.22% greater than controls at 12 h and 7 day respectively. Cell adhesion was consistently reduced above doses of 0.25 Gy. Transcriptional alterations were identified at these same doses in multiple genes related to proliferation, migration, and adhesion. Overall, this research began to elucidate the functional changes that occur in lens cells following radiation exposure, thereby providing a better mechanistic understanding of radiation-induced cataract development.

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“…Various genes are involved in the migration of LECs [ 19 , 21 , 33 , 34 , 35 ]. We observed an increased expression of MMP9 at 1 and 2 Gy ( Figure 6 A).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Radiation-Induced Alterations in Proliferation, Migration, and Adhesion in Lens Epithelial Cells and Implications for Cataract Development

et al. 2022

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“…ECM is known to acquire substantial changes in composition, structure, and mechanics, which together exacerbate disease etiology through promotion of angiogenesis, cell differentiation, and immune activation (Shelef et al., ). Fn was verified to activate FAK consequently promote HLEC cell migration (Liu et al., ). Our results showed that with STAT3 and NF‐κB pathways inhibitors, the migration rate of RPE cells induced by IL‐2 dropped as well.…”

Section: Discussionmentioning

confidence: 99%

Interleukin‐2 induces extracellular matrix synthesis and TGF‐β2 expression in retinal pigment epithelial cells

Jing

Wen

et al. 2019

Dev Growth Differ

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Macular fibrosis is a vital obstacle of vision acuity improvement of age‐related macular degeneration patients. This study was to investigate the effects of interleukin 2 (IL‐2) on epithelial‐mesenchymal transition (EMT), extracellular matrix (ECM) synthesis and transforming growth factor β2 (TGF‐β2) expression in retinal pigment epithelial (RPE) cells. 10 μg/L IL‐2 was used to induce fibrosis in RPE cells for various times. Western blot was used to detect the EMT marker α‐smooth muscle actin (α‐SMA), ECM markers fibronectin (Fn) and type 1 collagen (COL‐1), TGF‐β2, and the activation of the JAK/STAT3 and NF‐κB signaling pathway. Furthermore, JAK/STAT3 and NF‐κB signaling pathways were specifically blocked by WP1066 or BAY11‐7082, respectively, and the expression of α‐SMA, COL‐1, Fn and TGF‐β2 protein were detected. Wound healing and Transwell assays were used to measure cell migration ability of IL‐2 with or without WP1066 or BAY11‐7082. After induction of IL‐2, the expressions of Fn, COL‐1, TGF‐β2 protein were significantly increased, and this effect was correlated with IL‐2 treatment duration, while α‐SMA protein expression did not change significantly. Both WP1066 and BAY11‐7082 could effectively downregulate the expression of Fn, COL‐1 and TGF‐β2 induced by IL‐2. What's more, both NF‐κB and JAK/STAT3 inhibitors could suppress the activation of the other signaling pathway. Additionally, JAK/STAT3 inhibitor WP1066 and NF‐κB inhibitor BAY 11‐7082 could obviously decrease RPE cells migration capability induced by IL‐2. IL‐2 promotes cell migration, ECM synthesis and TGF‐β2 expression in RPE cells via JAK/STAT3 and NF‐κB signaling pathways, which may play an important role in proliferative vitreoretinopathy.

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“…Transforming growth factor β (TGF β ) and especially TGF- β 2, the major isoform in the aqueous humor of the eye, play a central role in the pathogenesis of PCO [15, 16]. In our study, treatment with TGF- β 2 at a concentration of 10 ng/ml for 48 h was used to stimulate SRA01/04 cells [17, 18], mimicking PCO in vitro.…”

Section: Discussionmentioning

confidence: 99%

LncRNA FEZF1-AS1 Promotes TGF-β2-Mediated Proliferation and Migration in Human Lens Epithelial Cells SRA01/04

Wang

Chen

et al. 2019

Journal of Ophthalmology

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Posterior capsule opacification (PCO) is a common complication after cataract surgery attributed to the proliferation and migration of postoperative residual lens epithelial cells (LECs). The long noncoding RNA (lncRNA) FEZ family zinc finger 1 antisense RNA 1 (FEZF1-AS1) promotes the proliferation and migration of multiple types of cancer cells. Here, we discovered that FEZF1-AS1 is markedly upregulated in TGF-β2-treated SRA01/04 cells. In addition, the proliferation and migration of SRA01/04 cells were enhanced following TGF-β2 treatment. FEZF1-AS1 knockdown inhibited the TGF-β2-induced proliferation and migration of SRA01/04 cells. Accordingly, FEZF1-AS1 overexpression promoted the TGF-β2-induced proliferation and migration of SRA01/04 cells. Finally, FEZF1-AS1 upregulated TGF-β2-induced SRA01/04 cell proliferation and migration via boosting FEZF1 protein levels. Our findings indicate that the dysregulation of FEZF1-AS1 participates in the TGF-β2-induced proliferation and migration of human lens epithelial cells (HLECs), which might be achieved, at least in part, through the induction of FEZF1 expression.

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The role of focal adhesion kinase in transforming growth factor-β2 induced migration of human lens epithelial cells

Cited by 4 publications

References 49 publications

Radiation-Induced Alterations in Proliferation, Migration, and Adhesion in Lens Epithelial Cells and Implications for Cataract Development

Radiation-Induced Alterations in Proliferation, Migration, and Adhesion in Lens Epithelial Cells and Implications for Cataract Development

Interleukin‐2 induces extracellular matrix synthesis and TGF‐β2 expression in retinal pigment epithelial cells

LncRNA FEZF1-AS1 Promotes TGF-β2-Mediated Proliferation and Migration in Human Lens Epithelial Cells SRA01/04

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