The Role of Fibronectin in Neural Development

Rogers, Sherry L.; Letourneau, Paul C.; Pech, Irene V.

doi:10.1159/000111904

Cited by 43 publications

(30 citation statements)

References 49 publications

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“…Fibronectin is also a n axon growth-promoting factor, but it may not provide guidance for growing axons (Rogers et al, 1989). During initial olfactory axon outgrowth (E12-E13), fibronectin is ubiquitously distributed throughout the cranial mesenchyme, but there is no specific pattern of this molecule associated with the olfactory nerve.…”

Section: Discussionmentioning

confidence: 99%

Expression of extracellular matrix molecules and cell surface molecules in the olfactory nerve pathway during early development

1996

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The spatial and temporal expression patterns of several extracellular matrix molecules--laminin and fibronectin and cell surface molecules, neural cell adhesion molecule (NCAM), L1, tenascin, chondroitin sulfate proteoglycan, and peanut agglutinin (PNA) binding sites--were investigated during early olfactory nerve development. NCAM and L1 have similar patterns: They are expressed in the olfactory nerve and on the olfactory receptor neurons (ORNs) commencing with the earliest olfactory axon outgrowth (E12-E15). Their expression patterns suggest that both NCAM and L1 are associated with extension and fasciculation of olfactory axons. A comparison of L1 and olfactory marker protein suggests that L1 is expressed predominantly on immature ORNs. Laminin has an unique punctate staining pattern in the developing olfactory pathway as early as E12. These laminin puncta might play a role in olfactory neurite outgrowth and guidance. At E14, when pioneer olfactory axons enter the brain, the laminin-positive meninges on the surface of the olfactory bulb primordium break down but remain intact in the rest of the telencephalon. This suggests a functional interaction between the olfactory axons and the glial-pial barrier. Fibronectin staining is diffuse throughout the cranial mesenchyme but is absent from the olfactory nerve pathway. No specific patterns of tenascin or chondroitin sulfate, were observed during early olfactory development. PNA binding sites were associated with olfactory axon fasciculation. The expression of several extracellular matrix molecules and cell surface molecules is spatially and temporally regulated in the developing olfactory system. These molecules, thus, may play functional roles in olfactory axon outgrowth, fasciculation, and/or guidance.

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Section: Discussionmentioning

confidence: 99%

Expression of extracellular matrix molecules and cell surface molecules in the olfactory nerve pathway during early development

1996

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“…An understanding of the mechanisms by which these afferent connections are constructed will require additional study. However, it is of interest to note that fibronectin-like protein, a substrate adhesion molecule known to be important in neurite guidance (34), is transiently expressed within the extracellular spaces below gerbil IHCs and OHCs from birth to P4 (35), the time period during which these nonspecific afferent arbors are forming.…”

Section: Discussionmentioning

confidence: 99%

Developmental segregation in the afferent projections to mammalian auditory hair cells.

Echteler

1992

Proc. Natl. Acad. Sci. U.S.A.

120

152

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The ammalian ear contains two types of auditory receptors, inner and outer hair cells, that lie in dose proximity to each other within the sensory epithelium of the cochlea. In adult mammals, these two classes of auditory hair cells are innervated by separate populations ofafferent neurons that differ strikingly in their cellular morphology and their pattern of arborization within the cochlea. At present, it Is unclear when or how these disinctive patterns of cochlear innervation emerge and become segregated during development. In the present study, an in vitro horseradish peroxidase labeling method was used to examine the formation of individual auditory neuron arbors at the same location within the apex of the developing gerbil cochlea. At birth, most cochlear neurons displayed peripheral arbors that embraced both inner and outer hair cell receptors. During the next 6 days, however, the arbors of individual cochlear afferents become confined to either the inner or outer hair cell zone, and thus there is a complete segregation of afferent innervation. This neural segregation occurs principally through the withdrawal of inappropriate connectimns to the outer hair cell ystem and is completed well before hearing commences.A central goal of neurobiology is to determine how developing neurons choose their specific synaptic partners. An excellent system for examining this issue is the mammalian auditory endorgan, the cochlea, since its pattern of innervation is among the most precise of any sensory or motor system. The adult cochlea contains two types of auditory receptors, termed inner (IHCs) and outer (OHCs) hair cells, which receive separate and highly distinctive patterns of afferent innervation. Ninety percent of all cochlear afferents project to individual IHCs via thick unbranched radial afferents, each of which terminates in a single synapse. The OHCs, on the other hand, receive a more diffuse innervation from a relatively few thin spiral afferents, each of which projects for hundreds of micrometers along the length of cochlea before branching to contact from 5 to 50 receptorsUltrastructural studies have provided evidence for substantial ontogenic changes in the form, number, and spatial arrangement of synapses between mammalian auditory receptors and cochlear nerve fibers (9-16). To understand the process by which these synaptic patterns are adjusted during development, however, it is necessary to examine the early morphological changes that occur in the formation of indi- In the present investigation, I have employed an in vitro horseradish peroxidase (HRP) method that permits labeling of small populations of auditory nerve fibers at any location within the developing cochlea. The Mongolian gerbil was selected for study since this species displays a prolonged period of postnatal cochlear maturation. In addition, the gerbil cochlea, like that of the mouse (26), lengthens <10% after birth, from an average of 10.8 mm in the newborn (unpublished data) to 12.1 mm in the adult (27), which greatly reduces ...

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“…Besides non-specific substrate adhesivity , morphoregulatory molecules are important regulators of neurite growth. Extracellular matrix glycoproteins, such as laminin, tenascin, janusin, and fibronectin, previously analyzed in detail for their influence on neurite growth of hippocampal neurons (Lochter et al, 1991;Lochter and Schachner, 1993;Lochter et al, 1994), are well known for their positive effects on promotion of axonal outgrowth from all neuronal cell types tested to date (Rathjen, 1988;Rogers et al, 1989;Beck et al, 1990;Chiquet-Ehrismann, 199 I). In contrast, dendritic growth is either not affected or even hindered by the presence of extracellular matrix molecules (Chamak and Prochiantz, 1989a;Lein and Higgins, 1989;Lein et al, 1991Lein et al, , 1992Osterhout et al, 1992;Lochter and Schachner, 1993).…”

Section: Neuritogenesis On Substrate-bound Morphoreguiatory Moleculesmentioning

confidence: 99%

Control of neuronal morphology in vitro: Interplay between adhesive substrate forces and molecular instruction

Lochter

Taylor

Braunewell

et al. 1995

J of Neuroscience Research

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Among the factors which influence neuronal morphology, the degree of substrate adhesivity has been suggested to play an important role in the growth and guidance of neurites. The present study was undertaken to investigate apparently contradictory results relating substrate adhesivity to the extent of neurite outgrowth. By using substrates coated with different concentrations of polyornithine to vary adhesivity, we could show that intermediate levels of neuron-to-substrate adhesive strength favored neurite outgrowth more than substrates of high or low adhesivity. However, when neurons were plated on substrates derived from the extracellular matrix, the strength of neuron-to-substrate adhesion was important for the growth of dendrite-like minor neurites, but not for the extension of axon-like major neurites, which grew independently of adhesive forces. On substrates of the cell adhesion molecule L1, growth of both major and minor neurites was adhesion-independent. Finally, in the presence of tenascin added to the culture medium, neurite growth was inhibited irrespective of the adhesivity of the substrate and the presence of substrate-bound extracellular matrix molecules or L1. These observations suggest that intermediate forces of adhesivity favor neurite growth in general, but that purely adhesive forces can be dominated by specific molecular instructions which differentially affect growth of major and minor neurites in positive and negative ways.

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The Role of Fibronectin in Neural Development

Cited by 43 publications

References 49 publications

Expression of extracellular matrix molecules and cell surface molecules in the olfactory nerve pathway during early development

Expression of extracellular matrix molecules and cell surface molecules in the olfactory nerve pathway during early development

Developmental segregation in the afferent projections to mammalian auditory hair cells.

Control of neuronal morphology in vitro: Interplay between adhesive substrate forces and molecular instruction

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