The Role of Extracellular Vesicles Secreted From Thermal Stress-Induced Adipose-Derived Stem Cells on Bone Regeneration

Akdeniz-Doğan, Zeynep D.; Sendur, Samet; Karademir-Yilmaz, Betul; Bugdayci, Onur; Çilingir‐Kaya, Özlem Tuğçe; Yilmaz-Goler, Ayse Mine; Gündüz, Oğuzhan; Çelebïler, Özhan

doi:10.1097/scs.0000000000007901

Cited by 3 publications

(3 citation statements)

References 37 publications

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“…By changing the ambient temperature of cultured cells (42°C and 37°C), it was found that the expression of exosome surface markers CD9 and CD63 in the 42°C group was more significant. It was found that the effect of using heat stress induced adipose-derived stem cell (ADSC) exosomes and using β-tricalcium phosphate (TCP) as carrier for bone regeneration was better than using β-TCP or using ADSCs and β-TCP alone [ 19 ]. Some studies have even collected exosome containing chemokines from heat-stressed adipocytes through the lipid raft-dependent pathway to attract and activate DC and T cells and induce an effective anti-tumor immune response as a new direction of tumor vaccine development.…”

Section: Temperaturementioning

confidence: 99%

Review of the Role of Mesenchymal Stem Cells and Exosomes Derived from Mesenchymal Stem Cells in the Treatment of Orthopedic Disease

2022

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Exosomes can be derived from mesenchymal stem cells (MSCs) and have recently been used to treat defects in the tendons. They may also have applications in treating osteoporosis. MSC-derived exosomes communicate with mesenchymal and osteogenic cells through endocrine or paracrine mechanisms and contribute factors involved in physiological and pathological orthopedic conditions associated with hypoxia and bone tumors. Also, generalized medical conditions, such as obesity, hyperglycemia, and degenerative diseases, can inhibit the osteogenic effect of MSC-derived exosomes. This review aims to present an update on the roles of MSCs and exosomes derived from MSCs in treating orthopedic diseases, such as osteoporosis, and in the repair of cartilage, tendons, and bone fractures.

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Section: Temperaturementioning

confidence: 99%

Review of the Role of Mesenchymal Stem Cells and Exosomes Derived from Mesenchymal Stem Cells in the Treatment of Orthopedic Disease

2022

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“…By changing the ambient temperature of cultured cells (42°C and 37°C), it was found that the surface markers CD9 and CD63 were more significantly expressed in the 42°C group. And that bone regeneration experiments using heat stress-induced ADSC-Exo with β-TCP (tricalcium phosphate) as a carrier were more effective than using β-TCP alone or using the ADSC with β-TCP ( Akdeniz-Dogan et al, 2021 ).…”

Section: Regulators Of Msc-exos Productionmentioning

confidence: 99%

MSC-Exos: Important active factor of bone regeneration

Ren

Lin

Liu

et al. 2023

Front. Bioeng. Biotechnol.

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Bone defect and repair is a common but difficult problem in restorative and reconstructive surgery. Bone tissue defects of different sizes caused by different reasons bring functional limitations and cosmetic deformities to patients. Mesenchymal stem cells (MSC), a major hotspot in the field of regeneration in recent years, have been widely used in various studies on bone tissue regeneration. Numerous studies have shown that the bone regenerative effects of MSC can be achieved through exosome-delivered messages. Although its osteogenic mechanism is still unclear, it is clear that MSC-Exos can directly or indirectly support the action of bone regeneration. It can act directly on various cells associated with osteogenesis, or by carrying substances that affect cellular activators or the local internal environment in target cells, or it can achieve activation of the osteogenic framework by binding to materials. Therefore, this review aims to summarize the types and content of effective contents of MSC-Exos in bone regeneration, as well as recent advances in the currently commonly used methods to enable the binding of MSC-Exos to the framework and to conclude that MSC-Exos is effective in promoting osteogenesis.

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“…Studies investigating exosomes derived from uMSCs that have been pre-differentiated down the osteogenic lineage in vitro prior to EV harvest, also induce bone regeneration when seeded into rat radial bone defect (158). EVs obtained from adipose tissue-derived MSCs (AD-MSCs) have successfully stimulated osteogenic differentiation in murine mouse models in vivo (159,160), although recent studies have indicated the regenerative potential of AD-MSC-EVs has similar potency to transplanted AD-MSCs (161). Recent in vitro studies have highlighted the ability of exosomes secreted by hAD-MSCs, combined with PLAbased scaffolds, to stimulate the osteogenic commitment of hAD-MSCs (162).…”

Section: Utilizing Evs For Bone Regenerative Medicinementioning

confidence: 99%

Identifying the Efficacy of Extracellular Vesicles in Osteogenic Differentiation: An EV-Lution in Regenerative Medicine

et al. 2022

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Mesenchymal stromal cells (MSCs) have long been the focus for regenerative medicine and the restoration of damaged or aging cells throughout the body. However, the efficacy of MSCs in cell-based therapy still remains unpredictable and carries with it enumerable risks. It is estimated that only 3-10% of MSCs survive transplantation, and there remains undefined and highly variable heterogeneous biological potency within these administered cell populations. The mode of action points to secreted factors produced by MSCs rather than the reliance on engraftment. Hence harnessing such secreted elements as a replacement for live-cell therapies is attractive. Extracellular vesicles (EVs) are heterogenous lipid bounded structures, secreted by cells. They comprise a complex repertoire of molecules including RNA, proteins and other factors that facilitate cell-to-cell communication. Described as protected signaling centers, EVs can modify the cellular activity of recipient cells and are emerging as a credible alternative to cell-based therapies. EV therapeutics demonstrate beneficial roles for wound healing by preventing apoptosis, moderating immune responses, and stimulating angiogenesis, in addition to promoting cell proliferation and differentiation required for tissue matrix synthesis. Significantly, EVs maintain their signaling function following transplantation, circumventing the issues related to cell-based therapies. However, EV research is still in its infancy in terms of their utility as medicinal agents, with many questions still surrounding mechanistic understanding, optimal sourcing, and isolation of EVs for regenerative medicine. This review will consider the efficacy of using cell-derived EVs compared to traditional cell-based therapies for bone repair and regeneration. We discuss the factors to consider in developing productive lines of inquiry and establishment of standardized protocols so that EVs can be harnessed from optimal secretome production, to deliver reproducible and effective therapies.

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The Role of Extracellular Vesicles Secreted From Thermal Stress-Induced Adipose-Derived Stem Cells on Bone Regeneration

Abstract: ¸ibu ¨yu ¨k Campus, Bas ¸ibu ¨yu ¨k Mah. Maltepe Bas ¸ibu ¨yu ¨k Yolu Sok. No: 9/2, Maltepe,

Cited by 3 publications

References 37 publications

Review of the Role of Mesenchymal Stem Cells and Exosomes Derived from Mesenchymal Stem Cells in the Treatment of Orthopedic Disease

Review of the Role of Mesenchymal Stem Cells and Exosomes Derived from Mesenchymal Stem Cells in the Treatment of Orthopedic Disease

MSC-Exos: Important active factor of bone regeneration

Identifying the Efficacy of Extracellular Vesicles in Osteogenic Differentiation: An EV-Lution in Regenerative Medicine

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