The role of extracellular vesicles in malaria biology and pathogenesis

Sampaio, Natália G.; Cheng, Lesley; Eriksson, Emily M.

doi:10.1186/s12936-017-1891-z

Cited by 57 publications

(65 citation statements)

References 65 publications

(96 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Extracellular vesicles are increasingly being recognised to play important roles in parasite cell–cell communication and interaction with the host (Mantel & Marti, ; Sampaio, Cheng, & Eriksson, ). Many parasite species produce EVs and employ these for modulation of host cells, which enhances pathogenesis (Szempruch et al, ; Twu et al, ) and/or inhibits immune responses (Buck et al, ; Silverman et al, ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Extracellular vesicles from early stagePlasmodium falciparum-infected red blood cells contain PfEMP1 and induce transcriptional changes in human monocytes

Sampaio

Emery

Garnham

et al. 2018

Cellular Microbiology

Self Cite

View full text Add to dashboard Cite

Pathogens can release extracellular vesicles (EVs) for cell-cell communication and host modulation. EVs from Plasmodium falciparum, the deadliest malaria parasite species, can transfer drug resistance genes between parasites. EVs from late-stage parasite-infected RBC (iRBC-EVs) are immunostimulatory and affect endothelial cell permeability, but little is known about EVs from early stage iRBC. We detected the parasite virulence factor PfEMP1, which is responsible for iRBC adherence and a major contributor to disease severity, in EVs, only up to 12-hr post-RBC invasion. Furthermore, using PfEMP1 transport knockout parasites, we determined that EVs originated from inside the iRBC rather than the iRBC surface. Proteomic analysis detected 101 parasite and 178 human proteins in iRBC-EVs. Primary human monocytes stimulated with iRBC-EVs released low levels of inflammatory cytokines and showed transcriptomic changes. Stimulation with iRBC-EVs from PfEMP1 knockout parasites induced more gene expression changes and affected pathways involved in defence response, stress response, and response to cytokines, suggesting a novel function of PfEMP1 when present in EVs. We show for the first time the presence of PfEMP1 in early stage P. falciparum iRBC-EVs and the effects of these EVs on primary human monocytes, uncovering a new mechanism of potential parasite pathogenesis and host interaction.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Extracellular vesicles are increasingly being recognised to play important roles in parasite cell-cell communication and interaction with the host (Mantel & Marti, 2014;Sampaio, Cheng, & Eriksson, 2017).…”

Section: Discussionmentioning

confidence: 99%

Extracellular vesicles from early stagePlasmodium falciparum-infected red blood cells contain PfEMP1 and induce transcriptional changes in human monocytes

Sampaio

Emery

Garnham

et al. 2018

Cellular Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…The release of EVs occurs either through direct budding from cell membranes (ectocytosis) or through the release of preformed vesicles from cellular compartments (exocytosis) (Sadallah et al, 2010). The systemic secretion of EVs is evolutionarily conserved among living organisms (Yanez-Mo' et al, 2015;Sampaio et al, 2017), and it is a constitutive cellular processes among protozoan parasites (Deolindo et al, 2013;Kehrer et al, 2016). Largely, the secretion of vesicles by parasitic protozoa maintains parasite-defensive mechanisms (Wowk et al, 2017), initiation of parasite infection and stronger interaction with host cells (Da Silveira et al, 1979;Ramirez et al, 2017;Moreira et al, 2019).…”

Section: Ppevs: Formation Characterization and Subcellular Originmentioning

confidence: 99%

Perils and Promises of Pathogenic Protozoan Extracellular Vesicles

Olajide

Cai

2020

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Extracellular vesicles (EVs) are membranous structures formed during biological processes in living organisms. For protozoan parasites, secretion of EVs can occur directly from the parasite organellar compartments and through parasite-infected or antigen-stimulated host cells in response to in vitro and in vivo physiological stressors. These secreted EVs characteristically reflect the biochemical features of their parasitic origin and activating stimuli. Here, we review the species-specific morphology and integrity of parasitic protozoan EVs in concurrence with the origin, functions, and internalization process by recipient cells. The activating stimuli for the secretion of EVs in pathogenic protozoa are discoursed alongside their biomolecules and specific immune cell responses to protozoan parasite-derived EVs. We also present some insights on the intricate functions of EVs in the context of protozoan parasitism.

show abstract

“…iRBC-derived EVs also mediate cell-cell communication between parasites. EVs internalized by iRBCs can transfer resistance markers and promote sexual differentiation, although the mechanistic details remain unknown (Mantel et al, 2013;Regev-Rudzki et al, 2013 andreviewed in Mantel andMarti, 2014;Sampaio et al, 2017). Communication between parasites extends beyond vesicle secretion.…”

Section: Sensing Each Othermentioning

confidence: 99%

Parasite Sensing of Host Nutrients and Environmental Cues

Zuzarte‐Luís

Mota

2018

Cell Host & Microbe

View full text Add to dashboard Cite

Parasites undergo complex life cycles that comprise a wide variety of cellular differentiation events in different host compartments and transmission across multiple hosts. As parasites depend on host resources, it is not surprising they have developed efficient mechanisms to sense alterations and adapt to the available resources in a wide range of environments. Here we provide an overview of the nutritional needs of different parasites throughout their diverse life stages and highlight recent insights into strategies that both hosts and parasites have developed to meet these nutritional requirements needed for defense, survival, and replication. These studies will provide the foundation for a systems-level understanding of host-parasite interactions, which will require the integration of molecular, epidemiologic, and mechanistic data and the application of interdisciplinary approaches to model parasite regulatory networks that are triggered by alterations in host resources.

show abstract

The role of extracellular vesicles in malaria biology and pathogenesis

Cited by 57 publications

References 65 publications

Extracellular vesicles from early stagePlasmodium falciparum-infected red blood cells contain PfEMP1 and induce transcriptional changes in human monocytes

Extracellular vesicles from early stagePlasmodium falciparum-infected red blood cells contain PfEMP1 and induce transcriptional changes in human monocytes

Perils and Promises of Pathogenic Protozoan Extracellular Vesicles

Parasite Sensing of Host Nutrients and Environmental Cues

Contact Info

Product

Resources

About