The role of extracellular matrix in the pathophysiology of diabetic wounds

Huang, Yaqing; Kyriakides, Themis R.

doi:10.1016/j.mbplus.2020.100037

Cited by 50 publications

(41 citation statements)

References 146 publications

(107 reference statements)

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“…Diabetes mellitus (DM) may impair wound healing, though the pathophysiology of impaired wound healing in DM is not fully understood ( Huang and Kyriakides, 2020 ). High blood glucose decreases collagen production and fibroblast migration and inhibits inflammatory cell proliferation, leading to chronically non-healing ulcers ( Okonkwo and DiPietro, 2017 ).…”

Section: Collagen Prolidase and Wound Healingmentioning

confidence: 99%

PROLIDASE: A Review from Discovery to its Role in Health and Disease

Eni-Aganga

Lanaghan

Muthukumar

et al. 2021

Front. Mol. Biosci.

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Prolidase (peptidase D), encoded by the PEPD gene, is a ubiquitously expressed cytosolic metalloproteinase, the only enzyme capable of cleaving imidodipeptides containing C-terminal proline or hydroxyproline. Prolidase catalyzes the rate-limiting step during collagen recycling and is essential in protein metabolism, collagen turnover, and matrix remodeling. Prolidase, therefore plays a crucial role in several physiological processes such as wound healing, inflammation, angiogenesis, cell proliferation, and carcinogenesis. Accordingly, mutations leading to loss of prolidase catalytic activity result in prolidase deficiency a rare autosomal recessive metabolic disorder characterized by defective wound healing. In addition, alterations in prolidase enzyme activity have been documented in numerous pathological conditions, making prolidase a useful biochemical marker to measure disease severity. Furthermore, recent studies underscore the importance of a non-enzymatic role of prolidase in cell regulation and infectious disease. This review aims to provide comprehensive information on prolidase, from its discovery to its role in health and disease, while addressing the current knowledge gaps.

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Section: Collagen Prolidase and Wound Healingmentioning

confidence: 99%

PROLIDASE: A Review from Discovery to its Role in Health and Disease

Eni-Aganga

Lanaghan

Muthukumar

et al. 2021

Front. Mol. Biosci.

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“…Compromised ECM remodeling is a typical presentation of diabetic foot ulcers. In defective wound healing, the balance between ECM degradation, production and maturation tends to yield more degraded, non-soluble fibrils, resulting in a disorganized ECM network [ 44 ].…”

Section: Microscopic Organization Of Foot Fasciae and Their Possible Role In Diabetic Footmentioning

confidence: 99%

“…Alterations in ECM are well-documented changes which occur during the fibrotic disease process in diabetes mellitus (DM). However, there is a controversial situation between the in vivo and in vitro conditions that underlines the role of the functional environment that can determine a decrease in the integrity of the skin [ 44 ].…”

Section: Microscopic Organization Of Foot Fasciae and Their Possible Role In Diabetic Footmentioning

confidence: 99%

Diabetic Foot: The Role of Fasciae, a Narrative Review

Pirri

Fede

Pirri

et al. 2021

Biology

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Wound healing is an intricate, dynamic process, in which various elements such as hyperglycemia, neuropathy, blood supply, matrix turnover, wound contraction, and the microbiome all have a role in this “out of tune” diabetic complex symphony, particularly noticeable in the complications of diabetic foot. Recently it was demonstrated that the fasciae have a crucial role in proprioception, muscular force transmission, skin vascularization and tropism, and wound healing. Indeed, the fasciae are a dynamic multifaceted meshwork of connective tissue comprised of diverse cells settled down in the extracellular matrix and nervous fibers; each constituent plays a particular role in the fasciae adapting in various ways to the diverse stimuli. This review intends to deepen the discussion on the possible fascial role in diabetic wounds. In diabetes, the thickening of collagen, the fragmentation of elastic fibers, and the changes in glycosaminoglycans, in particular hyaluronan, leads to changes in the stiffness, gliding, and the distribution of force transmission in the fasciae, with cascading repercussions at the cellular and molecular levels, consequently feeding a vicious pathophysiological circle. A clear scientific perception of fascial role from microscopic and macroscopic points of view can facilitate the identification of appropriate treatment strategies for wounds in diabetes and create new perspectives of treatment.

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“…Single cell and collective cell migrations are dependent on ECM integrity [22]. Diabetes mellitus negatively remodulates the ECM and compromises ECM-cell and cell-to-cell communications [21,23].…”

Section: Lecs Migratory Proliferative and Differentiation Activitiesmentioning

confidence: 99%

Bioimage analysis of cell physiology of primary lens epithelial cells from diabetic and non-diabetic cataract patients

Haykin

Oscar

Dimitrova

et al. 2020

Biotechnology & Biotechnological Equipment

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Cataract is a multifactorial disease with increasing prevalence with age. Adult diabetics develop cataract earlier. Lens epithelial cells (LECs) exposed to oxidative stress (ROS), increased calcium deposit and membrane damage, undergo apoptosis, which results in lens opacification. Remaining LECs post-surgery leads to posterior capsular opacification (PCO). This study's aim was to investigate the physiological characteristics of LECs from cataractous diabetic and nondiabetic lenses. Leader cells migration from age-related cataracts started on day 5-7 and from type-2 diabetics on day 8-10. Differences were found in the collective migratory activity and colony formation. On day 22, the colonies formed by LECs from age-related cataracts were three times more, than those formed by diabetic LECs. DNA synthesis and FOXM1 expression occurred in 55.76% of age-related cataract LECs, but only in 33.45% of diabetic LECs. The highest level of reactive oxygen species (ROS) was found in diabetic LECs. Extracellular matrix calcification followed the same pattern as ROS. Among the main reasons for the development of age-related and diabetic cataracts is lens damage due to ROS release and elevated calcium levels. Diabetic LECs experience significantly lower in vitro migration and proliferative activities, compared to LECs from age-related cataracts. This is the first study of its kind in Bulgaria, contributing to the elucidation of the mechanisms of primary and secondary cataractogenesis in diabetic and nondiabetic adults.

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The role of extracellular matrix in the pathophysiology of diabetic wounds

Cited by 50 publications

References 146 publications

PROLIDASE: A Review from Discovery to its Role in Health and Disease

PROLIDASE: A Review from Discovery to its Role in Health and Disease

Diabetic Foot: The Role of Fasciae, a Narrative Review

Bioimage analysis of cell physiology of primary lens epithelial cells from diabetic and non-diabetic cataract patients

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