The role of excitatory amino acids and substance P in the mediation of the cough reflex within the nucleus tractus solitarii of the rabbit

Mutolo, Donatella; Bongianni, Fulvia; Fontana, Giovanni; Pantaleo, Tito

doi:10.1016/j.brainresbull.2007.07.001

Cited by 61 publications

(206 citation statements)

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“…It has already been shown that substance P microinjected into the caudal NTS markedly potentiates cough reflex responses (Mazzone et al, 2005;Mutolo et al, 2007) while 10 mM CP-99,994 microinjected into the same site abolishes them (Mutolo et al, 2008). Thus, we searched for a concentration of the NK 1 receptor antagonist that injected into the caudal NTS did not cause any antitussive effect under control conditions, but could abolish lisinopril-induced potentiation.…”

Section: Resultsmentioning

confidence: 99%

“…All efforts were made to minimize both the number of animals used and their suffering. Experimental procedures and details about the methods employed have previously been described Mutolo et al, 2007Mutolo et al, , 2008Mutolo et al, , 2009Mutolo et al, , 2010Mutolo et al, , 2012Mutolo et al, , 2013Mutolo et al, , 2014.…”

Section: Ethical Approvalmentioning

confidence: 99%

“…The histological control of pipette tracks and injection sites was performed as previously described (for details, see Mutolo et al, 2007Mutolo et al, , 2012. Medullary sections in which injection sites The diagram also shows the location of injection sites ( ) into some control regions where lisinopril did not cause appreciable changes in the pattern of breathing and cough responses.…”

Section: Histologymentioning

confidence: 99%

“…The average values of cough-related variables were taken as single measurements for subsequent statistical analysis (GraphPad Prism 5, La Jolla, CA, USA). On some occasions, an expiration reflex could occur as the first motor event in a cough epoch (Korpáš and Tomori, 1979;Widdicombe and Fontana, 2006 for further details see Tatar et al, 2008;Mutolo et al, 2007Mutolo et al, , 2008Mutolo et al, , 2009Mutolo et al, , 2012Cinelli et al, 2013). Expiration reflexes were not considered for data analysis.…”

Section: Data Collection and Analysismentioning

confidence: 99%

“…Since losartan and lisinopril cross the blood-brain barrier (Pediconi et al, 2005;Ranadive et al, 1992;Tan et al, 2005), a central action of these drugs on the cough reflex can be hypothesized especially at the level of the caudal nucleus tractus solitarii (NTS), the main central terminus of cough-related afferents (Kubin and Davies, 1995;Kubin et al, 2006;Mutolo et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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The cough reflex is upregulated by lisinopril microinjected into the caudal nucleus tractus solitarii of the rabbit

Cinelli

Bongianni

Pantaleo

et al. 2015

Respiratory Physiology & Neurobiology

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a b s t r a c tWe have previously shown that cough potentiation induced by intravenous administration of the AT 1 receptor antagonist losartan is lower than that induced by the ACE inhibitor lisinopril in anesthetized and awake rabbits. Since losartan and lisinopril cross the blood-brain barrier, their central action on the cough reflex can be hypothesized. Mechanical stimulation of the tracheobronchial tree and citric acid inhalation were used to induce cough reflex responses in pentobarbital sodium-anesthetized, spontaneously breathing rabbits. Bilateral microinjections (30-50 nl) of losartan (5 mM), lisinopril (1 mM), bradykinin (0.05 mM), HOE-140 (0.2 mM, a bradykinin B 2 receptor antagonist) and CP-99,994 (1 mM, an NK 1 receptor antagonist) were performed into the caudal nucleus tractus solitarii, the predominant site of termination of cough-related afferents. Lisinopril, but not losartan increased the cough number. This effect was reverted by HOE-140 or CP-99,994. Cough potentiation was also induced by bradykinin. The results support for the first time a central protussive action of lisinopril mediated by an accumulation of bradykinin and substance P.

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Section: Resultsmentioning

confidence: 99%

Section: Ethical Approvalmentioning

confidence: 99%

Section: Histologymentioning

confidence: 99%

Section: Data Collection and Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The cough reflex is upregulated by lisinopril microinjected into the caudal nucleus tractus solitarii of the rabbit

Cinelli

Bongianni

Pantaleo

et al. 2015

Respiratory Physiology & Neurobiology

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Suppression of the cough reflex byα₂-adrenergic receptor agonists in the rabbit

et al. 2013

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The α2-adrenergic receptor agonist clonidine has been shown to inhibit citric acid-induced cough responses in guinea pigs when administered by aerosol, but not orally. In contrast, oral or inhaled clonidine had no effect on capsaicin-induced cough and reflex bronchoconstriction in humans. In addition, intravenous administration of clonidine has been shown to depress fentanyl-induced cough in humans. We investigated the effects of the α2-adrenergic receptor agonists, clonidine and tizanidine, on cough responses induced by mechanical and chemical (citric acid) stimulation of the tracheobronchial tree. Drugs were microinjected (30–50 nL) into the caudal nucleus tractus solitarii (cNTS) and the caudal ventral respiratory group (cVRG) as well as administered intravenously in pentobarbital sodium-anesthetized, spontaneously breathing rabbits. Bilateral microinjections of clonidine into the cNTS or the cVRG reduced cough responses at 0.5 mmol/L and abolished the cough reflex at 5 mmol/L. Bilateral microinjections of 0.5 mmol/L tizanidine into the cNTS completely suppressed cough responses, whereas bilateral microinjections of 5 mmol/L into the cVRG only caused mild reductions in them. Depressant effects on the cough reflex of clonidine and tizanidine were completely reverted by microinjections of 10 mmol/L yohimbine. Intravenous administration of clonidine (80–120 μg/kg) or tizanidine (150–300 μg/kg) strongly reduced or completely suppressed cough responses. These effects were reverted by intravenous administration of yohimbine (300 μg/kg). The results demonstrate that activation of α2-adrenergic receptors in the rabbit exerts potent inhibitory effects on the central mechanism generating the cough motor pattern with a clear action at the level of the cNTS and the cVRG.

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Sensory Pathways and Neural Modulation of Cough

Poliaček

2020

Cough: Pathophysiology, Diagnosis and Treatment

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The role of excitatory amino acids and substance P in the mediation of the cough reflex within the nucleus tractus solitarii of the rabbit

Cited by 61 publications

References 39 publications

The cough reflex is upregulated by lisinopril microinjected into the caudal nucleus tractus solitarii of the rabbit

The cough reflex is upregulated by lisinopril microinjected into the caudal nucleus tractus solitarii of the rabbit

Suppression of the cough reflex byα₂-adrenergic receptor agonists in the rabbit

Sensory Pathways and Neural Modulation of Cough

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The role of excitatory amino acids and substance P in the mediation of the cough reflex within the nucleus tractus solitarii of the rabbit

Cited by 61 publications

References 39 publications

The cough reflex is upregulated by lisinopril microinjected into the caudal nucleus tractus solitarii of the rabbit

The cough reflex is upregulated by lisinopril microinjected into the caudal nucleus tractus solitarii of the rabbit

Suppression of the cough reflex byα2-adrenergic receptor agonists in the rabbit

Sensory Pathways and Neural Modulation of Cough

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Product

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Suppression of the cough reflex byα₂-adrenergic receptor agonists in the rabbit