The role of ethylene in somatic embryogenesis from leaf discs of Coffea canephora

Hatanaka, Tomoko; Sawabe, Eiko; Azuma, Tetsushi; Uchida, Naotsugu; Yasuda, Takeshi

doi:10.1016/0168-9452(95)04103-2

Cited by 59 publications

(33 citation statements)

References 15 publications

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“…B.S.P. Endogenous ethylene has been reported to promote somatic embryogenesis in Daucus carota L. (Nissen 1994) and Coffea canephora (Hatanaka et al 1995). It has been suggested that ethylene modulates cotyledon expansion in microspore-derived embryos of Brassica napus (Hays et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Disturbance of ethylene biosynthesis and perception during somatic embryogenesis in Medicago sativa L. reduces embryos’ ability to regenerate

Kępczyńska

Zielińska

2011

Acta Physiol Plant

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Effects of non-specific ethylene biosynthesis inhibitors: salicylic acid (SA) and aminoethoxyvinylglycine (AVG), and of specific inhibitors of ethylene binding to receptors: 1-methylcyclopropene (1-MCP) and 2,5-norbornadiene (NBD) applied during proliferation and differentiation phases of indirect somatic embryogenesis (SE) of Medicago sativa L. cv. Rangelander on embryogenic suspension growth, embryo production, development, and ability to germinate and convert were studied. Application of SA and AVG alone or together at concentrations from 1 to 500 lM in B 5 g liquid medium during the proliferation phase had an inhibitory effect on ethylene production and embryogenic suspension growth. Additionally, it caused a drastic reduction in production of embryos and their development on BOi2Y solid differentiation medium. The inhibitory effect of SA was more visible than that of AVG. In addition, disturbance of ethylene biosynthesis during the proliferation phase of SE resulted in diminished lateral germination and conversion of cotyledonary embryos on MS solid medium. Moreover, blocking of ethylene receptors by 1-MCP during the proliferation phase also inhibited ethylene production and embryogenic suspension growth and reduced embryo production during differentiation. MCP almost completely inhibited development of cotyledonary embryos. At the same time, development of more embryos was arrested at the globular stage, and the number of abnormal embryos almost doubled. Similarly, addition of 1-MCP or NBD to the ambient atmosphere during the differentiation phase evidently arrested the development of embryos and, consequently, their ability to germinate and convert on MS regeneration medium. All the results presented above demonstrated that not only ethylene biosynthesis, but also ethylene action is involved in the control of individual phases of SE in Medicago sativa L. cv.Rangelander. And what is more, disturbance of these processes during distinct phases of SE adversely affects vigor of the somatic embryos obtained.

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Section: Introductionmentioning

confidence: 99%

Disturbance of ethylene biosynthesis and perception during somatic embryogenesis in Medicago sativa L. reduces embryos’ ability to regenerate

Kępczyńska

Zielińska

2011

Acta Physiol Plant

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“…For example, addition of ethylene inhibitors enhanced embryogeniccallus initiation in Zea mays (Vain et al 1989 ) and SE in Picea glauca (Kong and Yeung 1994 ), Hevea brasiliensis (Auboiron et al 1990 ). On contrary, SE has been shown to be stimulated by ethylene in M edicago sativa (Kepczynski et al 1992 ) and Coffea camephora (Hatanaka et al 1995 ). Moreover, in Daucus carota ethylene may either act as an inhibitor (Roustan et al 1990 ) or stimulator (Nissen 1994 ).…”

Section: Ethylenementioning

confidence: 84%

Genes and Trans-Factors Underlying Embryogenic Transition in Plant Soma-Cells

Pandey

Chaudhary

2015

Advances in the Understanding of Biological Sciences Using Next Generation Sequencing (NGS) Approaches

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Reproduction is a unique feature of all living beings in order to maintain the species existence. In plant kingdom, majorly pollination, fertilization, embryo development and its germination are the natural practices to persist in the species management. Many plants choose alternate mechanisms for their genetic succession either parallel or independently. For example, in the genus Bryophyllum plantlets develop on the fringes of leaves whereas fragments of the plant bodies of liverworts and mosses regenerate through embryo formation. In many Citrus species, nucellar cells produce embryos, perhaps for overcoming the fertilization barrier and higher growth competency. Theoretically it is understood that all plant cells are totipotent and can give rise complete plantlet. If so, why do different taxa, cultivars and type of explants (tissues) exhibit signifi cant variation in the regeneration potential? It is evident that different plant species respond in vitro with a wide spectrum of regeneration methods including organogenesis and somatic embryogenesis. The latter has drawn more attention in recent past as limited success had been achieved across plant species. Somatic EmbryogenesisSomatic embryogenesis (SE) is one of the most diverged modes of regeneration in plants. Somatic embryogenesis was fi rst reported in carrot cell suspension cultures (Steward et al. 1958 ). Somatic embryogenesis is the process of transition of a

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“…Based on the previous studies, ethylene is known to act positively during somatic embryogenesis in many species such as Coffea canephora [63], Oncidium sp. [64], Medicago sativa [65], Pinus sylvestris [66], and Quercus ilex [67].…”

Section: Ethylene-inducible Genesmentioning

confidence: 99%

Progress in understanding the regulation and expression of genes during plant somatic embryogenesis: A review

2018

J App Biol Biotech

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Based on the previous available documents involving molecular events during plant somatic embryogenesis, this report aims to review the advances that have been made for the past several years in the area of molecular mechanism of plant somatic embryogenesis. To begin with, studies suggest that the induction and differentiation of embryos from somatic tissue directly or through callusing involves the interaction of various cellular and molecular factors. Several intra-and extra-cellular proteins such as germins and germins-like proteins, lipid transfer proteins, heatshock proteins, and late embryogenesis abundant proteins are known to regulate the induction of somatic embryos from the somatic cell. Simultaneously, regulation and expression of specific genes such as housekeeping genes OsIAA in rice; hormone-responsive genes Dcarg-1, Dchsp-1, DcECP31, DcEMB1 in carrot; and AtECP63, Mt somatic embryo-related factor 1 in arabidopsis have been identified to play key roles during the process of somatic embryogenesis. These genes are known to express differentially for synthesis of new proteins during induction and development of somatic embryo. In addition, several transcription factors such as leafy cotyledon genes, agamouslike15 (AGL15) gene, ethylene-responsive element-binding protein (EREBPs), knotted1-like homeobox proteins, and RWP-RK group of plant-specific transcription factors are equally known that efficiently control the molecular events of somatic embryogenesis. Further, it is also now established that epigenetic factors such asDNA methylation, histone deacetylation/methylation, and microRNAs also influence the molecular mechanism of plant somatic embryogenesis.

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The role of ethylene in somatic embryogenesis from leaf discs of Coffea canephora

Cited by 59 publications

References 15 publications

Disturbance of ethylene biosynthesis and perception during somatic embryogenesis in Medicago sativa L. reduces embryos’ ability to regenerate

Disturbance of ethylene biosynthesis and perception during somatic embryogenesis in Medicago sativa L. reduces embryos’ ability to regenerate

Genes and Trans-Factors Underlying Embryogenic Transition in Plant Soma-Cells

Progress in understanding the regulation and expression of genes during plant somatic embryogenesis: A review

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