The role of estrogen in the developmental appearance of sensory-motor behaviors in the zebrafish (Danio rerio): The characterization of the “listless” model

Nelson, Bryan P.; Henriet, R; Holt, Andrew; Bopp, Katherine C.; Houser, Alexander; Allgood, Ottie E.; Turner, James

doi:10.1016/j.brainres.2008.05.049

Cited by 29 publications

(25 citation statements)

References 30 publications

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“…The AI 4-hydroxy androstenedione (4-OH-A, Sigma) was used at a 50 μM concentration, and estrogen (17β-Estradiol, Sigma) at 10 nM as established previously [16]. Both were dissolved in 0.05% ethanol and diluted to the appropriate concentration with and egg rearing solution (ERS).…”

Section: Methodsmentioning

confidence: 99%

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The Relationship between Estrogen and Nitric Oxide in the Prevention of Cardiac and Vascular Anomalies in the Developing Zebrafish (Danio Rerio)

et al. 2016

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It has been known that both estrogen (E2) and nitric oxide (NO) are critical for proper cardiovascular system (CVS) function. It has also been demonstrated that E2 acts as an upstream effector in the nitric oxide (NO) pathway. Results from this study indicate that the use of a nitric oxide synthase (NOS) inhibitor (NOSI) which targets specifically neuronal NOS (nNOS or NOS1), proadifen hydrochloride, caused a significant depression of fish heart rates (HR) accompanied by increased arrhythmic behavior. However, none of these phenotypes were evident with either the inhibition of endothelial NOS (eNOS) or inducible NOS (iNOS) isoforms. These cardiac arrhythmias could also be mimicked by inhibition of E2 synthesis with the aromatase inhibitor (AI), 4-OH-A, in a manner similar to that of nNOSI. In both scenarios, by using an NO donor (DETA-NO) in either NO + nNOSI or E2 + AI co-treatments, fish could be significantly rescued from decreased HR and increased arrhythmias. However, the addition of an NOS inhibitor (L-NAME) to the E2 + AI co-treatment fish prevented the rescue of low heart rates and arrhythmias, which strongly implicates the NO pathway as a downstream E2 targeted molecule for the maintenance of healthy cardiomyocyte contractile conditions in the developing zebrafish. Cardiac arrhythmias could be mimicked by the S-nitrosylation pathway inhibitor DTT (1,4-dithiothreitol) but not by ODQ (1H-[1–3]oxadiazolo[4,3-a]quinoxalin-1-one), the inhibitor of the NO receptor molecule sGC in the cGMP-dependent pathway. In both the nNOSI and AI-induced arrhythmic conditions, 100% of the fish expressed the phenotype, but could be rapidly rescued with maximum survival by a washout with dantrolene, a ryanodine Ca2+ channel receptor blocker, compared to the time it took for rescue using a control salt solution. In addition, of the three NOS isoforms, eNOS was the one most implicated in the maintenance of an intact developing fish vascular system. In conclusion, results from this study have shown that nNOS is the prominent isoform that is responsible, in part, for maintaining normal heart rates and prevention of arrhythmias in the developing zebrafish heart failure model. These phenomena are related to the upstream stimulatory regulation by E2. On the other hand, eNOS has a minimal effect and iNOS has little to no influence on this phenomenon. Data also suggests that nNOS acts on the zebrafish cardiomyocytes through the S-nitrosylation pathway to influence the SR ryanidine Ca2+ channels in the excitation-coupling phenomena. In contrast, eNOS is the prominent isoform that influences blood vessel development in this model.

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Section: Methodsmentioning

confidence: 99%

“…Previous work in our lab has described the “listless” model’ which is initiated by E2 and NO deprivation [13,16,17]. Specifically, both E2 and NO deficiencies cause reduced heart rates and increased arrhythmias, as well as initiating severe sensory-motor deficits.…”

Section: Introductionmentioning

confidence: 99%

The Relationship between Estrogen and Nitric Oxide in the Prevention of Cardiac and Vascular Anomalies in the Developing Zebrafish (Danio Rerio)

et al. 2016

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“…Reproductive steroids have both organizational and activational effects on motor behavior, and estrogen is necessary for the proper development of critical nervous system sensorimotor behaviors (214). Specifically, exogenous administration of estrogen to prenatal rodents results in increased spontaneous motor activity in adulthood (77), suggesting that estrogen has organizational effects on sensorimotor development.…”

Section: Behaviors Examples Too Numerous To Comprehensively Cite Incmentioning

confidence: 99%

Reproductive Steroid Regulation of Mood and Behavior

Schiller

Johnson

Abate

et al. 2016

Comprehensive Physiology

147

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In this article, we examine evidence supporting the role of reproductive steroids in the regulation of mood and behavior in women and the nature of that role. In the first half of the article, we review evidence for the following: (i) the reproductive system is designed to regulate behavior; (ii) from the subcellular to cellular to circuit to behavior, reproductive steroids are powerful neuroregulators; (iii) affective disorders are disorders of behavioral state; and (iv) reproductive steroids affect virtually every system implicated in the pathophysiology of depression. In the second half of the article, we discuss the diagnosis of the three reproductive endocrine-related mood disorders (premenstrual dysphoric disorder, postpartum depression, and perimenopausal depression) and present evidence supporting the relevance of reproductive steroids to these conditions. Existing evidence suggests that changes in reproductive steroid levels during specific reproductive states (i.e., the premenstrual phase of the menstrual cycle, pregnancy, parturition, and the menopause transition) trigger affective dysregulation in susceptible women, thus suggesting the etiopathogenic relevance of these hormonal changes in reproductive mood disorders. Understanding the source of individual susceptibility is critical to both preventing the onset of illness and developing novel, individualized treatments for reproductive-related affective dysregulation. © 2016 American Physiological Society. Compr Physiol 6:1135-1160, 2016e.

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“…Our data suggest that small fish could be very valuable models to address these important issues at the molecular, neuroanatomical and behavioural levels. For example, a recent study has documented the effects of early estrogen exposure on sensory-motor behaviors in zebrafish (Nelson et al, 2008).…”

Section: Early Functions For Estrogen Receptorsmentioning

confidence: 99%

Early regulation of brain aromatase (cyp19a1b) by estrogen receptors during zebrafish development

Mouriec

Lareyre

Tong

et al. 2009

Developmental Dynamics

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The role of estrogen in the developmental appearance of sensory-motor behaviors in the zebrafish (Danio rerio): The characterization of the “listless” model

Cited by 29 publications

References 30 publications

The Relationship between Estrogen and Nitric Oxide in the Prevention of Cardiac and Vascular Anomalies in the Developing Zebrafish (Danio Rerio)

The Relationship between Estrogen and Nitric Oxide in the Prevention of Cardiac and Vascular Anomalies in the Developing Zebrafish (Danio Rerio)

Reproductive Steroid Regulation of Mood and Behavior

Early regulation of brain aromatase (cyp19a1b) by estrogen receptors during zebrafish development

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