The role of EphB4/ephrinB2 signaling in root repair after orthodontically-induced root resorption

Li, Tiancheng; Wang, Han; Liu, Ruojing; Wang, Xin; Huang, Li; Wu, Zuping; Yin, Xing; Zou, Shujuan; Duan, Peipei

doi:10.1016/j.ajodo.2020.07.035

Cited by 4 publications

(3 citation statements)

References 48 publications

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“…The augmented expression of the four aforesaid biomarkers confirmed the anabolic effect of intermittent PTH on cementoblasts; however, OPN was found to decline after 3 cycles of intermittent PTH treatment, which was contradictory to some previously‐reported findings (Li et al, 2021; Vasconcelos et al, 2014; Xu et al, 2019). This discrepancy could be justified.…”

Section: Discussioncontrasting

confidence: 92%

Effects of intermittent parathyroid hormone on cementoblast‐mediated periodontal repair

et al. 2022

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Objectives To investigate the effects of intermittent parathyroid hormone on cementoblast‐mediated periodontal repair in the context of orthodontic‐induced root resorption. Materials and Methods The rat model of orthodontic‐induced root resorption was established. Sixty rats were randomly allocated into the experiment group (n = 30) and the control group (n = 30), either receiving a daily subcutaneous injection of recombinant human PTH or placebo vehicle. Enzyme‐linked immunosorbent assay, Micro‐computed tomography, hematoxylin and eosin staining, and immunohistochemistry staining were performed to detect the periodontal repair. In vitro, OCCM‐30 cells were exposed to intermittent PTH (incubated with PTH for the first 6 h in each 24‐h cycle). After three cycles, flow cytometry assay, alkaline phosphatase staining, and Alizarin red staining were performed. Quantitative real‐time polymerase chain reaction and Western blotting were employed to further determine the effects of intermittent PTH. Results Intermittent PTH‐responsive repair enhancement was detected with the expression of bone sialoprotein, osteocalcin, collagen‐1, and alkaline phosphatase significantly upregulated. Increased expressions of cementoblastic proteins were positively correlated to cycles of PTH administration. The proportion of cementoblasts in S and G2/M phases was increased; namely, intermittent PTH promoted cementoblast cell proliferation. Conclusions Intermittent parathyroid hormone administration promotes cementoblast‐mediated cementogenesis during periodontal repair in a time‐dependent manner.

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Section: Discussioncontrasting

confidence: 92%

Effects of intermittent parathyroid hormone on cementoblast‐mediated periodontal repair

et al. 2022

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“…Huang et al (2020) showed that modulating the balance of the ephB4-ephrinB2 axis could improve the characteristics of osteoporosis. EphB4-ephrinB2 signaling affects the osteoclastic factors RANK ligand/osteoprotegerin (Li et al, 2021). Differential outcomes of ephB4-ephrinB2 signaling may offer formidable challenges for the development of RA therapeutics.…”

Section: Discussionmentioning

confidence: 99%

Yi Shen Juan Bi Pill alleviates bone destruction in inflammatory arthritis under postmenopausal conditions by regulating ephrinB2 signaling

Li²,

Cao³

et al. 2022

Front. Pharmacol.

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Yi Shen Juan Bi Pill (YSJB) is a traditional Chinese medicine (TCM) formulation that has a therapeutic effect upon rheumatoid arthritis (RA), but how YSJB affects bone destruction in arthritis under postmenopausal conditions is not known. We evaluated the therapeutic role of YSJB in bone destruction in postmenopausal arthritis, We used collagen-induced arthritis (CIA) rats who had been ovariectomized (OVX) as models and explored the possible mechanism from the synovium and bone marrow (BM). Arthritis was generated after ovariectomy or sham surgery for 12 weeks. After 14 days of primary immunization, rats were administered YSJB or estradiol valerate (EV) for 28 days. YSJB could prevent bone destruction in the inflamed joints of rats in the OVX + CIA group. CIA promoted osteoclast differentiation significantly in the synovial membrane according to tartrate resistant acid phosphatase (TRACP) staining, and OVX tended to aggravate the inflammatory reaction of CIA rats according to hematoxylin-and-eosin staining. Immunohistochemistry revealed that the synovium did not have significant changes in erythropoietin-producing hepatocellular interactor (ephrin)B2 or erythropoietin-producing hepatocellular (eph) B4 expression after YSJB treatment, but YSJB treatment reduced nuclear factor of activated T cells (NFATc)1 expression. The BM of rats in the OVX + CIA exhibited remarkable increases in the number of osteoclasts and NFATc1 expression, as well as significantly reduced expression of ephrinB2 and ephB4 compared with the CIA group and sham group. YSJB treatment reduced NFATc1 expression significantly but also increased ephrinB2 expression in the BM markedly. These data suggest that YSJB exhibit a bone-protective effect, it may be a promising therapeutic strategy for alleviating bone destruction in arthritis under postmenopausal conditions, and one of the mechanisms is associated with the modulation of ephrinB2 signaling.

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“…Therefore, the identification of potential CTSK inhibitors will facilitate the development of treatments for bone metabolic diseases. NVP-BHG712, which is as a specific EphB4 inhibitor, has attracted much attention because of its effects in inhibiting tumor metastasis (Becerikli et al, 2015;Li et al, 2021;Troster et al, 2018) . Angiogenesis plays a key role in bone formation and osteoporosis (Filipowska, Tomaszewski, Niedźwiedzki, Walocha, & Niedźwiedzki, 2017;Ramasamy, Kusumbe, Wang, & Adam A recent study reported that NVP-BHG712 inhibited angiogenesis (You et al, 2017) .…”

Section: Ctskmentioning

confidence: 99%

NVP-BHG712 against postmenopausal osteoporosis in mice by targeting Cathepsin K

Zhao

Liu

et al. 2022

Preprint

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Background and Purpose Recent evidence suggests that postmenopausal osteoporosis is associated with increased bone resorption function in osteoclasts, of which Cathepsin K is a key molecule. In this study, we aimed to screen small molecule compounds targeting Cathepsin K and evaluate whether they affect osteoclast bone resorption function. Experimental Approach We screened out the small-molecule compound NVP-BHG712 targeting CTSK by molecular docking, and studied its pharmacological effect on bone resorption function of osteoclasts. To this end, we evaluated bone mass changes in postmenopausal mice by μCT, ELISA, and H&E staining. In addition, we also investigated the effects of NVP-BHG712 on osteoclast differentiation, bone resorption function and expression of osteoclast differentiation related factors in vitro. Key Results Surprisingly, we found that oral NVP-BHG712 treatment significantly reduced bone loss in postmenopausal mice. In vitro osteoclast culture, it was found that this effect was achieved by inhibiting osteoclast differentiation and bone resorption. Meanwhile, NVP-BHG712 significantly decreased the expression of genes related to osteoclast differentiation, including CTSK, MMP9, CTR, IP3R1, IP3R3, and OC-STAMP. Conclusion and Implications The present findings suggest that NVP-BHG712 reduces bone resorption function by inhibiting osteoclast differentiation, and is a potential drug for preventing and treating postmenopausal osteoporosis and other diseases.

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The role of EphB4/ephrinB2 signaling in root repair after orthodontically-induced root resorption

Cited by 4 publications

References 48 publications

Effects of intermittent parathyroid hormone on cementoblast‐mediated periodontal repair

Effects of intermittent parathyroid hormone on cementoblast‐mediated periodontal repair

Yi Shen Juan Bi Pill alleviates bone destruction in inflammatory arthritis under postmenopausal conditions by regulating ephrinB2 signaling

NVP-BHG712 against postmenopausal osteoporosis in mice by targeting Cathepsin K

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