The role of EphA7 in different tumors

Chen, Xiangyi; Yu, De-Chen; Zhou, Hai-Yu; Zhang, Xiaobo; Hu, Yi-Cun; Zhang, Ruihao; Gao, Xi-Dan; Lin, Mo‐Jun; Guo, Taowen; Zhang, Kun

doi:10.1007/s12094-022-02783-1

Cited by 7 publications

(5 citation statements)

References 135 publications

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“…EPHA7, a member of the Eph receptor family, is widely involved in intercellular signal transduction, regulating cell proliferation and differentiation. 42 However, its expression on trophoblast cells and the regulation by 1,25(OH) 2 D 3 have not been reported. CRLF1, a member of the cytokine type I receptor family, is upregulated by pro-inflammatory cytokines such as tumor necrosis factor α and interleukin 6, 43 which indicated that CRLF1 may be involved in the regulation of the immune system in the inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome‐wide profile of 1α,25 dihydroxyvitamin D₃ in HTR‐8/SVneo cells

Luo

Zhang

et al. 2023

J of Obstet and Gynaecol

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Aim Vitamin D3 has been implicated in multiple reproductive events, whereas the effect of its bioactive metabolite 1α, 25 dihydroxyvitamin D3 (1,25(OH) 2D3) on transcriptome profile of the placenta is unclear. The aim of this article is to determine transcriptome‐wide profile caused by 1,25(OH) 2D3 in human placental trophoblast cells. Methods We performed RNA sequencing after stimulation of HTR‐8/SVneo cells with 0.1, 1, 10, and 100 nM 1,25(OH)2D3 for 24 h, identified differentially expressed genes by edgeR package (version 3.38.4), and analyzed Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways by webtool Metascape. Also, common genes and specific genes in different concentrations of 1,25(OH) 2D3 were identified. Results There were 180, 158, 161, and 174 differentially expressed genes after 0.1, 1, 10, and 100 nM 1,25(OH) 2D3 stimulation, respectively. KEGG pathway analysis displayed that “lipid and atherosclerosis” were significantly enriched at 0.1 and 1 nM 1,25(OH)2D3, while “cytokine‐cytokine receptor interaction,” “TGF‐beta signaling pathway” and “hippo signaling pathway” were significantly enriched in 1, 10, and 100 nM 1,25(OH)2D3. CYP24A1 was a significantly expressed common gene. UCP3 was significantly expressed in low concentrations and might affect energy metabolism. TCF24, EIF3CL, ABCD2, EPHA7, CRLF1, and SECTM1 were specific genes at physiological concentration. Similarly, SPDYE1, IQUB, IL18R1, and ZNF713 were considered as specific genes at supraphysiological concentration. Conclusions 1,25(OH)2D3 mainly affected the expression of CYP24A1 gene in HTR‐8/SVneo cells. Specific genes accounted for the majority of differentially expressed genes at different concentrations. However, their functions need to be further confirmed.

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Section: Discussionmentioning

confidence: 99%

“…However, the actual function of ABCD2 in vivo is unclear. EPHA7, a member of the Eph receptor family, is widely involved in intercellular signal transduction, regulating cell proliferation and differentiation 42 . However, its expression on trophoblast cells and the regulation by 1,25(OH) 2 D 3 have not been reported.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome‐wide profile of 1α,25 dihydroxyvitamin D₃ in HTR‐8/SVneo cells

Luo

Zhang

et al. 2023

J of Obstet and Gynaecol

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“…31 EPHA7 is a member of the proteintyrosine kinase family and plays a dual part of an oncogene or tumor suppressor in intercellular signal transduction and the regulation of cell proliferation and differentiation as well as resistance to chemotherapy drugs. 32,33 Besides, down-regulation of SNCA and EPHA7…”

Section: Discussionmentioning

confidence: 99%

“…By reducing the activity of PI3K/AKT, this gene inhibits the proliferation of lung adenocarcinoma cells 31 . EPHA7 is a member of the protein‐tyrosine kinase family and plays a dual part of an oncogene or tumor suppressor in intercellular signal transduction and the regulation of cell proliferation and differentiation as well as resistance to chemotherapy drugs 32,33 . Besides, down‐regulation of SNCA and EPHA7 genes has been associated with multidrug resistance in OC 34 .…”

Section: Discussionmentioning

confidence: 99%

“… 31 EPHA7 is a member of the protein‐tyrosine kinase family and plays a dual part of an oncogene or tumor suppressor in intercellular signal transduction and the regulation of cell proliferation and differentiation as well as resistance to chemotherapy drugs. 32 , 33 Besides, down‐regulation of SNCA and EPHA7 genes has been associated with multidrug resistance in OC. 34 The PI3K/AKT signaling pathway is the most common changed signaling pathway in OC and dysregulation of this pathway has been identified in 70% of OC patients.…”

Section: Discussionmentioning

confidence: 99%

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Meta‐analysis of microarray data to determine gene indicators involved in the cisplatin resistance in ovarian cancer

Hashemi Sheikhshabani,

Amini‐Farsani,

Kazemifard

et al. 2023

Cancer Reports

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BackgroundSignificant miss‐expressed gene indicators contributing to cisplatin resistance in ovarian cancer have not been completely understood. It seems that several regulatory genes and signaling pathways are associated with the emergence of the chemo‐resistant phenotype.AimsHere, a meta‐analysis approach was adopted to assess deregulated genes involved in relapse after the first line of chemotherapy (cisplatin).Methods and ResultsTo do so, six ovarian cancer libraries were gathered from GEO repository. Batch effect removal and quality assessment, and boxplots and PCA were performed using SVA and ggplot2 packages in R, respectively. Cisplatin‐resistant and ‐sensitive ovarian cancer groups were compared with find genes with significant expression changes using linear regression models in the LIMMA R package. The significance threshold for DEGs was taken as adj p‐value < .05 and − 1 > logFC > 1. A total of 261 genes were identified to have significant differential expression levels in the cisplatin‐resistant versus cisplatin‐sensitive group. Among the 10 top up‐regulated and down‐regulated genes, PITX2, SNCA, and EPHA7 (up), as well as TMEM98 (down) are indirect upstream regulators of PI3K/AKT signaling pathway, contributing greatly to the development of chemo‐resistance in cancer via promoting cell proliferation, survival, and cell cycle progression as well as inhibiting apoptosis. Moreover, a comprehensive assessment of DEGs revealed the dysregulation of not only membrane ion channels KCa1.1, Kv4, and CACNB4, affecting cell excitability, proliferation, and apoptosis but also cell adhesion proteins COL4A6, EPHA3, and CD9, affecting the attachment of normal cells to ECM and apoptosis, introducing good options to reverse cisplatin resistance.ConclusionOur results predict and suggest that upstream regulators of PI3K/AKT signaling pathway, ion channels, and cell adhesion proteins play important roles in cisplatin resistance development in ovarian cancer.

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