The role of eosinophils in asthma

Alenzi, Faris Q.; Alanazi, Fahad G. B.; Al-Faim, Abdulaziz D.; Al-Rabea, Mohamed W.; Tamimi, Waleed; Tarakji, Bassel; Kujan, Omar; Al-Jabri, Ali A.; Wyse, Richard K.H.

doi:10.4236/health.2013.52a045

Cited by 1 publication

(1 citation statement)

References 42 publications

(33 reference statements)

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“…The rates of activity of these molecular switches are determined by factors like guanine exchange factors(GEFs), which catalyze Rap/Ras proteins to GTP-bound active state and GTPase activating proteins(GAPs) which enhance the intrinsic hydrolysis of bound GTP to GDP. 5 Ras-like GTPase proteins regulate important signalling pathways such as mitogen activated protein kinase (MAPK) MAPK/ERK and PI3K 6 and such regulation is determined by the effector proteins with which they interact and Raf-1 is one of the most important effector molecules for Ras family of proteins 7 . Raf is the first kinase in the MAPK cascade and interacts with Ras-like GTPases via the Ras binding domain (RBD).…”

Section: Introductionmentioning

confidence: 99%

Phosphorylation of interfacial phosphosite leads to increased binding of Rap-Raf complex

Devanand

Ghosh

Venkatraman

et al. 2021

Preprint

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The effect of phosphorylation of a serine residue in the Rap protein, residing at the complex interface of Rap-Raf complex is studied using atomistic molecular dynamics simulations. As the phosphosite of interest (SER39) is buried at the interface of the Rap-Raf complex, phosphorylation of only Rap protein was simulated and then complexed with the RBD of Raf for further analysis of complex stability. Our simulations reveal that the phosophorylation increases the binding of complex through strong electrostatic interactions and changes the charge distribution of the interface significantly. This is manifested as an increase in stable salt-bridge interactions between the Rap and Raf of the complex. Network analysis clearly shows that the phosphorylation of SER39 reorganizes the community network to include the entire region of Raf chain, including, Raf L4 loop potentially affecting downstream signalling.

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Section: Introductionmentioning

confidence: 99%