The Role of Endothelin System in Renal Structure and Function during the Postnatal Development of the Rat Kidney

Borghese, María Florencia Albertoni; Ortiz, Mariela; Balonga, Sabrina; Szokalo, Rocío Moreira; Majowicz, Mónica P.

doi:10.1371/journal.pone.0148866

Cited by 5 publications

(7 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Renal function is influenced by direct and indirect action of endothelin. There is evidence that endothelin has an important role in rat renal postnatal development ( 21 ).…”

Section: Discussionmentioning

confidence: 99%

Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children

Hashemi¹,

Sadeghi‐Bojd²,

Aryanezhad³

et al. 2016

J Nephropathol

View full text Add to dashboard Cite

Background: Primary nephrotic syndrome (NS) is a common kidney disease in children. Objectives: The present study was aimed to investigate whether rs5370 G>T (lys198Asn) genetic variant of endothelin-1 (ET-1) is involved in the susceptibility to NS. Patients and Methods: This case-control study was performed on 138 patients with NS and 150 healthy children. Genomic DNA was extracted from whole blood using salting out method. Polymorphism of the ET-1 rs5370 G>T (lys198Asn) polymorphism detected by T-ARMS-PCR as well as PCR-RFLP method. Results: The results showed that the genotype and allelic frequencies of the ET-1 rs5370 G>T variant were not significantly different between cases and controls. Furthermore, subgroup analysis showed that rs5370 G>T variant was not associated with gender of patients. In NS patients the genotype was not associated with cholesterol, triglyceride, total protein and albumin levels. Conclusions: In conclusion, our findings indicate that ET-1 rs5370 G>T is not associated with NS. Further studies with larger sample sizes and different ethnicities are required to validate our findings.

show abstract

“…Renal function is influenced by direct and indirect action of endothelin. There is evidence that endothelin has an important role in rat renal postnatal development ( 21 ).…”

Section: Discussionmentioning

confidence: 99%

Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children

Hashemi¹,

Sadeghi‐Bojd²,

Aryanezhad³

et al. 2016

J Nephropathol

View full text Add to dashboard Cite

show abstract

“…In this paper we show that adult male SD rats that had been treated with a dual ERA during their early postnatal period have a 20% increase in their blood pressure after 8 days consuming a high sodium diet. We had shown in a previous paper that the inhibition of the ET system with a dual ERA produces a decrease in nephron number [9]. Although low nephron number is not always associated with hypertension, offspring with diminished nephron number are more susceptible to a second insult or adverse impact [40].…”

Section: Discussionmentioning

confidence: 97%

“…Newborn rats were treated daily from postnatal day 1 to postnatal day 20 with vehicle (distilled water) or with Bosentan (Actelion, 20 mg/kg/day), a dual ERA, which was administered orally with a micropipette. Blockade of ET receptors was performed during the first 20 days of life, comprising all the lactation period, since in rats growth and maturation of the kidney also continue after the completion of nephrogenesis and it has been considered that nephrons reach terminal differentiation at the time of weaning [9].…”

Section: Animals and Treatmentsmentioning

confidence: 99%

“…However, the role of ET system during the postnatal period is not completely understood. We have shown previously that the inhibition of ET system in the rat with a dual ET receptor antagonist (ERA) during the early postnatal period affects both renal structure and function, decreasing the number of glomeruli, the juxtamedullary filtration surface area and the glomerular filtration rate and increasing the proteinuria, being these effects more pronounced in male rats [9]. It is widely accepted that a reduced glomerular number predisposes to hypertension and to kidney disease in the adulthood [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inhibition of Endothelin system during the postnatal nephrogenic period in the rat. Its relationship with hypertension and renal disease in adulthood

et al. 2020

Self Cite

View full text Add to dashboard Cite

The aim of this work was to study the effect of a high sodium (HS) diet on blood pressure and renal function in male adult rats that have been treated with a dual Endothelin receptor antagonist (ERA) during their early postnatal period (day 1 to 20 of life). Male Sprague-Dawley rats were divided in four groups: C NS : control rats with normosodic diet; ERA NS : ERAtreated rats with normosodic diet; C HS : control rats with high sodium diet; ERA HS : ERAtreated rats with HS diet. Systolic blood pressure (SBP) was recorded before and after the diet and 24-hour metabolic cage studies were performed. AQP2 and α-ENac expressions were measured by western blot and real time PCR in the renal medulla. Vasopressin (AVP) pathway was evaluated by measuring V2 receptor and adenylyl cyclase 6 (AC6) expression and cAMP production in the renal medulla. Pre-pro ET-1mRNA was also evaluated in the renal medulla. Only rats that had been treated with an ERA during their postnatal period increased their SBP after consumption of a HS diet, showing an impaired capacity to excrete sodium and water, i.e. developing salt sensitivity. This salt sensitivity would be mediated by an increase in renomedullary expression and activity of AQP2 and α-ENaC as a consequence of increased AC6 expression and cAMP production and/or a decreased ET-1 production in the renal medulla. The knowledge of the molecular mechanisms underlying the perinatal programming of salt sensitive hypertension will allow the development of reprogramming strategies in order to avoid this pathology.

show abstract

“…A subset of these organs is shown in Figure S2. In the rat, the process of nephrogenesis continues in the immediate postnatal period [13]. We therefore examined the development of the kidney in Csf1rko rats in detail.…”

Section: The Effect Of the Csf1rko On Major Organsmentioning

confidence: 99%

CSF1R-dependent macrophages control postnatal somatic growth and organ maturation

Keshvari

Caruso

Batoon

et al. 2020

Preprint

View full text Add to dashboard Cite

The development of the mononuclear phagocyte system (MPS) is controlled by signals from the CSF1 receptor (CSF1R). Homozygous mutation of the Csf1r locus (Csf1rko) in inbred rats led to the loss of non-classical monocytes and tissue macrophage populations, reduced postnatal somatic growth, severe developmental delay impacting all major organ systems and early mortality. The developmental impacts overlap with growth hormone/insulin-like growth factor (GH/IGF1) deficiency and Csf1rko rats lacked circulating IGF1. The liver is the main source of circulating IGF1. Expression profiling of juvenile wild-type and Csf1rko livers identified 2760 differentially expressed genes associated with the loss of macrophages, severe hypoplasia, delayed hepatocyte maturation, disrupted lipid metabolism and dysregulation of the GH/IGF1 system. Transfer of WT bone marrow at weaning restored circulating IGF1 and reversed the mutant phenotypes enabling long term survival and fertility. Phenotypic rescue was achieved without reconstituting blood monocytes or CSF1-responsive bone marrow progenitors. The results demonstrate that CSF1R-dependent macrophages control the growth and maturation of the liver and regulate the GH/IGF1 axis.

show abstract

The Role of Endothelin System in Renal Structure and Function during the Postnatal Development of the Rat Kidney

Cited by 5 publications

References 47 publications

Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children

Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children

Inhibition of Endothelin system during the postnatal nephrogenic period in the rat. Its relationship with hypertension and renal disease in adulthood

CSF1R-dependent macrophages control postnatal somatic growth and organ maturation

Contact Info

Product

Resources

About