The role of endothelin pathway in modulation of airway reactivity to methacholine in C57Bl/6 and BALB/c mice

Landgraf, Richardt Gama; Jancar, Sônia

doi:10.1016/j.ejphar.2008.06.015

Cited by 3 publications

(5 citation statements)

References 17 publications

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“…This is in accordance with a previous publication [16] in which we found that the non-selective antagonist of endothelin receptors (Bosentan), significantly reduced (56%) the lung concentration of endothelins. In both studies ETs were measured in homogenates of lung tissue of C57Bl/6.…”

Section: Discussionsupporting

confidence: 94%

“…This indicates that there is a basal production of endothelins in the lung and that there is a turnover of endothelin synthesis and degradation, where endothelins exert a positive feedback on their synthesis. However, this effect seems to be strain-dependent since in the previous study this feedback was not observed in BALB/c mice [16]. Surprisingly, the concentration of endothelins in whole lung homogenates did not increase after antigen challenge of sensitized mice, compared to control mice (sensitized and not challenged) or to naive mice.…”

Section: Discussioncontrasting

confidence: 49%

“…DMSO has to be used because these antagonists are insoluble in aqueous solution at this concentration according to Brochu et al [15]. The concentration of the antagonists was chosen from previous studies [16][17][18][19]. …”

Section: Drug Treatmentsmentioning

confidence: 99%

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Endothelin A receptor antagonist modulates lymphocyte and eosinophil infiltration, hyperreactivity and mucus in murine asthma

Landgraf

Jancar

2008

International Immunopharmacology

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Section: Discussionsupporting

confidence: 94%

Section: Discussioncontrasting

confidence: 49%

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Endothelin A receptor antagonist modulates lymphocyte and eosinophil infiltration, hyperreactivity and mucus in murine asthma

Landgraf

Jancar

2008

International Immunopharmacology

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Section: Discussionmentioning

confidence: 99%

“…Previous comparisons of responsiveness between C57BL/6 and BALB/c mice were also performed in lung isolated perfusion system ( Held and Uhlig, 2000 ; Landgraf and Jancar, 2008 ). While the lung from BALB/c mice was more responsive than the lung from C57BL/6 mice in one study ( Held and Uhlig, 2000 ), the other study showed the opposite ( Landgraf and Jancar, 2008 ). The increased responsiveness in the former was not restricted to methacholine, as the lung of BALB/c mice was also more responsive to endothelin-1 and the thromboxane A 2 analog U-46619 ( Held and Uhlig, 2000 ).…”

Section: Discussionmentioning

confidence: 99%

Smooth Muscle Hypocontractility and Airway Normoresponsiveness in a Mouse Model of Pulmonary Allergic Inflammation

et al. 2021

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The contractility of airway smooth muscle (ASM) is labile. Although this feature can greatly modulate the degree of airway responsiveness in vivo, the extent by which ASM’s contractility is affected by pulmonary allergic inflammation has never been compared between strains of mice exhibiting a different susceptibility to develop airway hyperresponsiveness (AHR). Herein, female C57BL/6 and BALB/c mice were treated intranasally with either saline or house dust mite (HDM) once daily for 10 consecutive days to induce pulmonary allergic inflammation. The doses of HDM were twice greater in the less susceptible C57BL/6 strain. All outcomes, including ASM contractility, were measured 24 h after the last HDM exposure. As expected, while BALB/c mice exposed to HDM became hyperresponsive to a nebulized challenge with methacholine in vivo, C57BL/6 mice remained normoresponsive. The lack of AHR in C57BL/6 mice occurred despite exhibiting more than twice as much inflammation than BALB/c mice in bronchoalveolar lavages, as well as similar degrees of inflammatory cell infiltrates within the lung tissue, goblet cell hyperplasia and thickening of the epithelium. There was no enlargement of ASM caused by HDM exposure in either strain. Unexpectedly, however, excised tracheas derived from C57BL/6 mice exposed to HDM demonstrated a decreased contractility in response to both methacholine and potassium chloride, while tracheas from BALB/c mice remained normocontractile following HDM exposure. These results suggest that the lack of AHR in C57BL/6 mice, at least in an acute model of HDM-induced pulmonary allergic inflammation, is due to an acquired ASM hypocontractility.

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The responses of pulmonary and systemic circulation and airway to anaphylactic mediators in anesthetized BALB/c mice

et al. 2016

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The role of endothelin pathway in modulation of airway reactivity to methacholine in C57Bl/6 and BALB/c mice

Cited by 3 publications

References 17 publications

Endothelin A receptor antagonist modulates lymphocyte and eosinophil infiltration, hyperreactivity and mucus in murine asthma

Endothelin A receptor antagonist modulates lymphocyte and eosinophil infiltration, hyperreactivity and mucus in murine asthma

Smooth Muscle Hypocontractility and Airway Normoresponsiveness in a Mouse Model of Pulmonary Allergic Inflammation

The responses of pulmonary and systemic circulation and airway to anaphylactic mediators in anesthetized BALB/c mice

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