“…Predicted peaks associated with each expression cluster were then further analyzed for known transcription factor motif enrichment, separately for cephalopod and ancient microsynteny (“Methods”, Supplementary Data 2 ). We find that the cephalopod microsynteny module 2, which is associated with multiple nervous tissues, was enriched for the transcription factors binding motifs Chop 26 , E2F1 27 , NeuroG2 28 , COUP-TFII 29 , Atf4 30 involved in nervous system differentiation and developmental transcription factors ZBTB18, Esrrb, Tcf21, Pitx1, GATA in all three developmental stages ( p < 1e−3). Module 4 was similarly enriched in transcription factor binding motifs involved in nervous system and general development such as Tcf3, TCFL2, GATA, Tcf21, and Pitx1 in all three developmental stages ( p < 1e−3).…”