The Role of DNA Polymerase I in Genetic Recombination and Viability of Escherichia coli

Smirnov, G. B.; Sinzinis, B.I.; Саенко, А.С.

doi:10.1007/978-1-4684-2895-7_55

Molecular Mechanisms for Repair of DNA 1975

DOI: 10.1007/978-1-4684-2895-7_55

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The Role of DNA Polymerase I in Genetic Recombination and Viability of Escherichia coli

G. B. Smirnov¹,

B.I. Sinzinis²,

А.С. Саенко³

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2000

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“…Okazaki fragments and fill in gaps generated by base excision repair and nucleotide excision repair (Smirnov et al, 1975). Pol III (encoded dnaE, dnaQ and holE), is the main replicative polymerase and is responsible for carrying out replication of the genome ).…”

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confidence: 99%

Use of Two-Dimensional Agarose-Gel Analysis to Characterize Processing of UV-Irradiated Plasmids and the Composition of the Replisome Following UV-induced Arrest

Jeiranian¹

2000

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In this thesis, I address two fundamental questions related to our understanding of how DNA damage is processed and repaired during replication. Using Two-dimensional (2-D) agarose gel analysis, I first examine whether DNA damage on plasmids introduced by transformation is processed in a manner similar to that observed on endogenously replicating plasmids and the chromosome. The original intent for using this approach was to develop a technique that could examine how different DNA adducts would be repaired in various sequence contexts. However, I found that distinct differences exist between the processing of DNA damage on transforming plasmids and the chromosome. The 2-D agarose gel analysis shows that RecA-mediated processing does not contribute to the survival of transforming plasmids and that this effect is likely due to inefficient replication of the plasmids after they are initially introduced into cells. These observations, while important, place limitations on the usefulness of transforming plasmids to characterize cellular repair processes. In a second question, I characterize the composition of the replisome following arrest by UV-induced DNA damage. Using 2-D agarose gel analysis the structural changes that occur in DNA during processing and repair have been well characterized, however, little is known about the fate of the replisome itself during these events. I used thermosensitive replication mutants to compare the

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mentioning

confidence: 99%

Use of Two-Dimensional Agarose-Gel Analysis to Characterize Processing of UV-Irradiated Plasmids and the Composition of the Replisome Following UV-induced Arrest

Jeiranian¹

2000

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The Role of DNA Polymerase I in Genetic Recombination and Viability of Escherichia coli

Cited by 1 publication

References 12 publications

Use of Two-Dimensional Agarose-Gel Analysis to Characterize Processing of UV-Irradiated Plasmids and the Composition of the Replisome Following UV-induced Arrest

Use of Two-Dimensional Agarose-Gel Analysis to Characterize Processing of UV-Irradiated Plasmids and the Composition of the Replisome Following UV-induced Arrest

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