The role of dioleoyl phosphatidylethanolamine in cationic liposome mediated gene transfer

Farhood, Hassan; Serbina, Natalya V.; Huang, Leaf

doi:10.1016/0005-2736(95)80016-9

Cited by 763 publications

(479 citation statements)

References 16 publications

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“…DOPE, which contains an ethanolamine head group, promotes transfection whereas a similar neutral phospholipid, dioleoylphosphatidylcholine (DOPC), which contains a choline head group, does not. 142,143 A critical factor accounting for this difference in transfection enhancing activity is that DOPE forms nonbilayer lipid structures whereas DOPC does not (Litzinger D and Huang. Biochim Biophys Acta 1992; 1113: 201).…”

Section: Endosomolysismentioning

confidence: 99%

Intracellular trafficking of nonviral vectors

2005

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Section: Endosomolysismentioning

confidence: 99%

Intracellular trafficking of nonviral vectors

2005

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“…24 The presence of DOPE greatly enhances the transfection efficiency and is thought to be the consequence of the non-bilayer forming properties of this lipid. 26 Several drawbacks of this transfection system have to be noted. (1) The DNA/lipid complexes formed are not stable in the presence of serum, 27 limiting the application of this system in vivo.…”

Section: Nmol) Were Mixed With Increasing Amounts Of Virosomes Contaimentioning

confidence: 99%

Gene transfer mediated by fusion protein hemagglutinin reconstituted in cationic lipid vesicles

et al. 1999

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Hemagglutinin, the membrane fusion protein of influenza erythrocyte ghosts. Efficient cell transfection of BHK-21 virus, is known to mediate a low-pH-dependent fusion cells was observed with virosomes containing 30 mol% reaction between the viral envelope and the limiting mem-DODAC and plasmid encoding for ␤-galactosidase (pCMV brane of the endosomal cell compartment following cellular ␤-gal) associated to their surface. The transfection activity uptake of the virus particles by receptor-mediated endoobserved was dependent on the functional activity of hemcytosis. Here we exploited this activity of hemagglutinin to agglutinin. Contrary to DNA/cationic lipid complexes the achieve efficient gene delivery to cultured cells. Hemagglutransfection was not dependent on the cationic lipid to DNA tinin was reconstituted in the presence of the monocationic charge ratio. Importantly, transfection of BHK-21 cells with lipid dioleoyldimethylammonium chloride (DODAC) to perpCMV ␤-gal by DODAC-containing virosomes did not show mit plasmid binding to the virosome surface. Virosomes any significant signs of cytotoxicity that is commonly with 30 mol% DODAC exhibited a distinct binding capacity observed with DNA/cationic lipid complexes. Together with for plasmid without causing aggregation. The virosome the high levels of expression of the transgene this highfusion activity was not affected by the cationic lipid DODAC lights the potential of DODAC-containing virosomes as a as demonstrated by low-pH-dependent lipid mixing with novel approach in nonviral gene transfer.

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“…In most cases, these lipids are mixed with an equimolar amount of a ''helper'' lipid. Most frequently, the neutral lipid DOPE is used, since this is known to enhance transfection efficiency (Felgner et al, 1994;Gao and Huang, 1995;Stegmann and Legendre, 1997;Zhou and Huang, 1994), due to its ability to facilitate the formation of cationic liposomes and to its tendency to form non-bilayer phases that may contribute to the destabilization of the endosomal membrane (Farhood et al, 1995;Felgner et al, 1994;Legendre and Szoka, 1992;Zhou and Huang, 1994). On the other hand, cholesterol (Chol) constitutes another promising ''helper'' lipid, since it has been demonstrated that high levels of transfection can be obtained both in vitro and in vivo, upon its association with a cationic lipid (Crook et al, 1998;Templeton et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Improving lipoplex-mediated gene transfer into C6 glioma cells and primary neurons

Cruz

Simões

Lima

2004

Experimental Neurology

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The development of methodologies for gene transfer into the central nervous system is crucial for gene therapy of neurological disorders. In this study, different cationic liposome formulations were used to transfer DNA into C6 glioma cells and primary hippocampal and cortical neurons by varying the nature of the helper lipid (DOPE, Chol) or a mixture of DOPE and cholesterol (Chol) associated to DOTAP. In addition, the effect of the lipid/DNA (+/À) charge ratio, the association of the ligand transferrin to the lipoplexes, and the stage of differentiation of the primary cells on the levels of transfection activity, transfection efficiency, and duration of gene expression were evaluated. Mechanistic studies were also performed to investigate the route of delivery of the complexes into neurons. Our results indicate that DOTAP:Chol (1:1 mol ratio) was the best formulation to transfer a reporter gene into C6 glioma cells, primary hippocampal neurons, and primary cortical neurons. The use of transferrin-associated lipoplexes resulted in a significant enhancement of transfection activity, as compared to plain lipoplexes, which can be partially attributed to the promotion of their internalization mediated by transferrin. While for hippocampal neurons the levels of luciferase gene expression are very low, for primary cortical neurons the levels of transgene expression are high and relatively stable, although only 4% of the cells has been transfected. The stage of cell differentiation revealed to be critical to the levels of gene expression. Consistent with previous findings on the mechanisms of cell internalization, the experiments with inhibitors of the endocytotic pathway clearly indicate that transferrin-associated lipoplexes are internalized into primary neurons by endocytosis. Promising results were obtained in terms of the levels and duration of gene expression, particularly in cortical neurons when transfected with the Tfassociated lipoplexes, this finding suggesting the usefulness of these lipid-based carriers to deliver genes within the CNS. D 2004 Elsevier Inc. All rights reserved.

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The role of dioleoyl phosphatidylethanolamine in cationic liposome mediated gene transfer

Cited by 763 publications

References 16 publications

Intracellular trafficking of nonviral vectors

Intracellular trafficking of nonviral vectors

Gene transfer mediated by fusion protein hemagglutinin reconstituted in cationic lipid vesicles

Improving lipoplex-mediated gene transfer into C6 glioma cells and primary neurons

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