2013
DOI: 10.1016/j.ibmb.2013.05.003
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The role of desaturases in the biosynthesis of marking pheromones in bumblebee males

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Cited by 25 publications
(38 citation statements)
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“…Yeast cells were harvested and total cellular lipids were extracted as described by Buček et al [34], and fatty acid methyl esters (FAMEs) were prepared according to Matoušková et al [35]. The resulting FAMEs were extracted with hexane (600 μl), and the extracts were analyzed by gas chromatography coupled to mass spectrometry (GC/MS) using the conditions described below.…”
Section: Methodsmentioning
confidence: 99%
“…Yeast cells were harvested and total cellular lipids were extracted as described by Buček et al [34], and fatty acid methyl esters (FAMEs) were prepared according to Matoušková et al [35]. The resulting FAMEs were extracted with hexane (600 μl), and the extracts were analyzed by gas chromatography coupled to mass spectrometry (GC/MS) using the conditions described below.…”
Section: Methodsmentioning
confidence: 99%
“…In insects, Δ9-desaturases serve in biosynthetic pathways towards cuticular hydrocarbons in the fruit fly Drosophila melanogaster 2324, scent-marking pheromones in male bumblebees25 and long-range sex pheromones in some female moths2627. However, in the latter group, molecular reconstructions of biosynthetic pathways revealed that the majority of chemical structures derive from the evolution of novel desaturase multigene families absent from other insect orders2728.…”
mentioning
confidence: 99%
“…In insects, desaturases have been extensively studied based on their role in chemical communication and potential to contribute to speciation. To date, a number of desaturase genes have been shown to be involved in pheromone biosynthesis in many insects including moths (Roelofs & Rooney, ; Buček et al ., ) and flies (Knipple et al ., ; Buček et al ., ). In these systems, the introduction of carbon–carbon double bonds at specific positions in pheromone precursors to generate pheromone components that vary in chain length, double‐bond number, double‐bond position and double‐bond orientation contributes to the structural diversity in pheromone structures that drive species specificity (Knipple et al ., ; Roelofs & Rooney, ).…”
Section: Discussionmentioning
confidence: 99%