The Role of Deoxyribonucleic Acid in Ribonucleic Acid Synthesis

Frederick, Elizabeth W.; Maitra, Umadas; Hurwitz, Jerard

doi:10.1016/s0021-9258(18)94446-7

Cited by 56 publications

(3 citation statements)

References 32 publications

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“…As has been observed for a variety of templates with RNA polymerase from E. coli (Maitra and Hurwitz, 1965), A. cinelandii (Krakow and Horsely, 1967), and M. luteus (Frederick et al, 1969) there is relatively greater total initiation (although still over 90% with ATP and GTP) and initiation by GTP relative to ATP increases. Increased total initiation indicates a shorter average chain length.…”

Section: Resultsmentioning

confidence: 56%

Transcription of chromatin. Initial and terminal nucleotides of ribonucleic acid synthesized by calf thymus and Escherichia coli ribonucleic acid polymerases

Keshgegian¹,

Garibian²,

Fürth³

1973

Biochemistry

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Section: Resultsmentioning

confidence: 56%

Transcription of chromatin. Initial and terminal nucleotides of ribonucleic acid synthesized by calf thymus and Escherichia coli ribonucleic acid polymerases

Keshgegian¹,

Garibian²,

Fürth³

1973

Biochemistry

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“…Similar properties include: a requirement for divalent metal ions (Mn2+ or Mg2+) and a primer RNA; a specificity for ATP as substrate; and attachment of the poly(A) product to the 3 '-hydroxyl end of the primer RNA. The failure of native or denatured DNA to serve as a primer for the yeast enzyme and the inhibition of AMP by the other 3 ribonucleoside triphosphates clearly distinguishes this enzyme from the yeast DNA-dependent RNA polymerase (Frederick et al, 1969) which also catalyzes a DNA-dependent synthesis of polyriboadenylate from ATP.…”

Section: Discussionmentioning

confidence: 99%

“…The assay for DNA-dependent RNA polymerase was carried out exactly as described by Frederick et al (1969) using [14C]ATP as the labeled ribonucleoside triphosphate and either native or alkali-denatured calf thymus DNA. The paper disk assay as previously described was used to determine any [1 AMP incorporation into product.…”

Section: Methodsmentioning

confidence: 99%

Properties of a polyriboadenylate polymerase isolated from yeast ribosomes

Js¹,

Bretthauer²

1971

Biochemistry

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Dynamics of interaction of RNA polymerase II with nucleosomes. I. Effect of salts

Bhargava

1993

Protein Science

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Mononucleosomes were labeled with the sulfhydryl-specific fluorescence probe 1,5-IAEDANS (5-(2-((iodoacety1)amino)ethyl)amino-naphthalene-1-sulfonic acid) by attaching the dye to the single cysteine of H3 through a covalent linkage. The enzyme RNA polymerase I1 (pol 11) utilized the native and the reconstituted fluorescent nucleosomes as templates with greatest efficiency when 0.2 M potassium acetate (AcOK) was used as the supporting salt; 0.2 M NaCl was found to be very much inhibitory. Measurement of polarity of the microenvironment of the dye at its binding site in the nucleosome showed the conformation to be more open in the presence of AcOK, compared to that in 0.1 or 0.2 M NaCl. The binding of pol I1 to the nucleosome resulted in a relatively more compact structure when measured in terms of the polarity of the microenvironment of the dye in various salt-dependent conformations of the nucleosomes. Time-resolved fluorescence spectroscopy showed that the probe molecule at its binding site undergoes certain excited-state processes, and the presence/absence or rate of these excited-state processes depends on the conformation of nucleosomes, which in turn depends on the type and concentration of the ion present in the medium. Time-resolved emission spectra showed that binding of nucleosomes by pol 11 established some new contacts that resulted in inaccessibility of the dye to the bulk solvent, reflecting a more hydrophobic environment for the dye in the steady-state spectra. Thus, binding or transcription of nucleosomes by pol I1 did not break open their structure. Rather, some transient internal adjustments within the histone octamer may take place to accommodate the bulky pol I1 molecule.

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The Role of Deoxyribonucleic Acid in Ribonucleic Acid Synthesis

Cited by 56 publications

References 32 publications

Transcription of chromatin. Initial and terminal nucleotides of ribonucleic acid synthesized by calf thymus and Escherichia coli ribonucleic acid polymerases

Transcription of chromatin. Initial and terminal nucleotides of ribonucleic acid synthesized by calf thymus and Escherichia coli ribonucleic acid polymerases

Properties of a polyriboadenylate polymerase isolated from yeast ribosomes

Dynamics of interaction of RNA polymerase II with nucleosomes. I. Effect of salts

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