The role of dendritic cells in allergic diseases

Liu, Peng; Kang, Chenglin; Zhang, Jin; Liu, Yue; Liu, Jiang‐Qi; Hu, Tian‐Yong; Zeng, Xianhai; Qiu, Shuqi

doi:10.1016/j.intimp.2022.109449

Cited by 12 publications

(8 citation statements)

References 103 publications

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“…It is generally believed that LPS polarizes DCs toward a Th1-promoting profile; the culture of BMDCs with LPS (1 μg/mL) resulted in a decreased expression of OX40L ( Figure 4 a), whereas TIM-4 expression was not affected ( Figure 4 b). Furthermore, the incubation of BMDCs with Mo-Pr also stimulated the production of CCL22 and CCL17 ( Figure 5 a,b), two chemokines capable of recruiting Th2 cells to the sites of allergic inflammation [ 24 ]. We performed allogeneic MLR.…”

Section: Resultsmentioning

confidence: 99%

The Potential Role of Moringa oleifera Lam. Leaf Proteins in Moringa Allergy by Functionally Activating Murine Bone Marrow-Derived Dendritic Cells and Inducing Their Differentiation toward a Th2-Polarizing Phenotype

Xi,

Li,

Liu

et al. 2023

Nutrients

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Moringa oleifera leaves are an inexpensive substitute for staple foods. Despite limited data, Moringa oleifera leaf protein (Mo-Pr) may be allergenic in BALB/c mice. In mouse models and allergic patients, dendritic cells (DCs) may be involved in food allergy. In addition, some allergens, including food allergens, can directly activate DCs and induce Th2 polarization. We investigated whether Mo-Pr can modulate the functional profile of murine bone marrow-derived dendritic cells (BMDCs) in vitro. BMDCs were obtained from mouse bone marrow cultured with granulocyte–macrophage colony-stimulating factor (GM-CSF) for 7 days and then treated with lipopolysaccharide (LPS) or Mo-Pr. BMDC phenotypes were evaluated via flow cytometry, cytokine production was assessed using ELISA, the expression of key genes was studied using qRT-PCR, the effects on T-cell differentiation were investigated using mixed lymphocyte reaction (MLR), and transcriptional changes in BMDCs were investigated using RNA-Seq. Mo-Pr-specific IgE was investigated in recipient serum after BMDC transfer. Mo-Pr treatment significantly induced BMDC maturation, increased the expression of CD80/86 and MHC II, resulted in the production of IL-12 and TNF-α, and induced T-cell differentiation. Mo-Pr treatment stimulated BMDCs’ expression of the Th2 promoters OX40L and TIM-4, induced the production of the Th2-type chemokines CCL22 and CCL17, and decreased the Th1/Th2 ratio in vitro. Healthy recipients of Mo-Pr-treated BMDCs produced Mo-Pr-specific IgE.

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Section: Resultsmentioning

confidence: 99%

The Potential Role of Moringa oleifera Lam. Leaf Proteins in Moringa Allergy by Functionally Activating Murine Bone Marrow-Derived Dendritic Cells and Inducing Their Differentiation toward a Th2-Polarizing Phenotype

Xi,

Li,

Liu

et al. 2023

Nutrients

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“…Previous studies suggested that DC and eosinophils played important roles in the pathogenesis and development of AR. 3 , 24 An increase in the number and activity of DC has been observed in patients with AR, especially in those with moderate-to-severe symptoms, which suggests an overactive immune response. 25 In addition, it has been demonstrated that DC can facilitate the differentiation of Th2 cells and stimulate the production of IgE, as well as facilitate the infiltration of eosinophils into nasal mucosal tissue.…”

Section: Discussionmentioning

confidence: 99%

“… 1 The main pathological feature of AR is T helper 2 (Th2)-type inflammation with eosinophil infiltration, which was primarily mediated by dendritic cells (DC) and macrophages. 2 , 3 Previous research has demonstrated that AR exhibits a high degree of clinical heterogeneity, with varying levels of disease severity observed among affected individuals, which poses significant challenges for clinical diagnosis, assessment, and treatment. 4 , 5 Despite previous publications finding that several indicators or biomarkers correlated with disease severity, their sensitivities and specificities were limited.…”

Section: Introductionmentioning

confidence: 99%

Soluble TAM Receptor Tyrosine Kinases Correlate with Disease Severity and Predict the Early Responsiveness of Sublingual Immunotherapy in Allergic Rhinitis

Zhou,

Feng,

Wen

et al. 2023

JIR

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Background Allergic rhinitis (AR) is a common allergic disease, and SLIT has shown effectiveness as a treatment method. This study focuses on the evaluation of serum TAM receptor tyrosine kinases (TYRO3, AXL, and MER) levels as potential indicators of disease severity and predictive markers for sublingual immunotherapy (SLIT) responsiveness in AR patients. Methods A total of 160 AR subjects, including 40 mild AR (MAR) and 120 moderate-severe AR (MSAR) patients, and 40 healthy controls (HC) were recruited. Serum concentrations of TYRO3, AXL, and MER were measured and their relationships with disease severity were examined. In the MSAR group, 102 patients underwent SLIT, and the early efficacy was evaluated. The correlations between the baseline serum concentrations of TYRO3, AXL, and MER and the early responsiveness of SLIT were analyzed. Results Serum concentrations of TYRO3, AXL, and MER were significantly reduced in AR patients, particularly in those MSAR subjects. Correlation analysis results indicated that serum TYRO3 and MER levels were negatively correlated with the visual analog scale (VAS) and the total nasal symptom score (TNSS). After one year of follow-up, 80 AR patients completed the treatment and were divided into effective and ineffective groups. Serum baseline levels of TYRO3 and MER were found to be lower in the effective group compared to the ineffective group. Additionally, there was a significant increase in serum TYRO3 and MER levels compared to baseline levels. Receiver operating characteristic (ROC) analysis revealed that circulating TYRO3 and MER had potential values for reflecting AR severity and predicting early SLIT responsiveness. Conclusion Serum TYRO3 and MER concentrations were decreased in AR patients and negatively associated with disease severity. Circulating TYRO3 and MER seem to be promising indicators for monitoring the efficacy of SLIT in AR patients.

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“…ARh 2 exhibits a complex pathology, characterized primarily by an excessive type 2 immune response, and mediated by type 2 innate lymphoid cells (ILC2s), 9 regulatory T-cells (Treg) cells 2 and dendritic (DC) cells, 10 which induce the production of various immune substances, such as cytokines and chemokines. 2,9 However, an accurate regulation mechanism remains to be fully established.…”

mentioning

confidence: 99%

Mite‐negative allergic rhinitis: A model of the regulation mechanism of atopy onset

Horiuchi

2024

Scand J Immunol

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The role of dendritic cells in allergic diseases

Cited by 12 publications

References 103 publications

The Potential Role of Moringa oleifera Lam. Leaf Proteins in Moringa Allergy by Functionally Activating Murine Bone Marrow-Derived Dendritic Cells and Inducing Their Differentiation toward a Th2-Polarizing Phenotype

The Potential Role of Moringa oleifera Lam. Leaf Proteins in Moringa Allergy by Functionally Activating Murine Bone Marrow-Derived Dendritic Cells and Inducing Their Differentiation toward a Th2-Polarizing Phenotype

Soluble TAM Receptor Tyrosine Kinases Correlate with Disease Severity and Predict the Early Responsiveness of Sublingual Immunotherapy in Allergic Rhinitis

Mite‐negative allergic rhinitis: A model of the regulation mechanism of atopy onset

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