The role of cytoskeleton, components of inositol phospholipid signaling pathway and iron in Ehrlichia canis in vitro proliferation

Levenhagen, Marcelo Arantes; Alves, Rosiane Nascimento; Rieck, Susana Elisa; Labruna, Marcelo Bahia; Beletti, Marcelo Emı́lio

doi:10.1007/s11259-012-9525-y

Cited by 5 publications

(2 citation statements)

References 12 publications

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“…This test used strain Bc05.10 and the test method was performed as described previously. 24 Verapamil, a calcium channel blocker, 39 was used to study the effect on the antifungal activity of SL. We chose 150.0 and 200.0 μg•mL −1 as the concentrations of Verapamil and 30.0 μg•mL −1 as the concentration of SL for this experiment.…”

Section: Reaction Assay Of Sl With Cna By Bio-layer Interferometry (Bli)mentioning

confidence: 99%

Crucial Role of the Ca²⁺/CN Signaling Pathway in the Antifungal Activity of Seselin against Botrytis cinerea

Wang

Hou

et al. 2023

J. Agric. Food Chem.

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Botrytis cinerea causes gray mold in many fruit and vegetable crops. We previously found that Seselin (SL) displayed antifungal activity against B. cinerea (EC50 = 6.1 μg·mL–1), and this study investigated the effects of Ca2+ and the Ca2+/CN signaling pathway on its antifungal activity against B. cinerea. The results indicated that exogenous Ca2+, Cyclosporine A, and Verapamil reduced the sensitivity of SL against B. cinerea; SL significantly reduced the intracellular Ca2+ concentration in the hyphae; the sensitivity of strains ΔbcCCH1 and ΔbcMID1 to SL were significantly increased; and the expressions of CCH1, MID1, CNA, PMC1, and PMR1 genes of the Ca2+/CN signaling pathway were significantly downregulated by SL treatment. Hence, SL is a potential compound for developing fungicides against B. cinerea. SL dramatically reduces intracellular Ca2+ concentration and disturbs Ca2+ homeostasis, leading to cell death. The Ca2+/CN signaling pathway plays an important role in the antifungal activity of SL against B. cinerea.

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Section: Reaction Assay Of Sl With Cna By Bio-layer Interferometry (Bli)mentioning

confidence: 99%

Crucial Role of the Ca²⁺/CN Signaling Pathway in the Antifungal Activity of Seselin against Botrytis cinerea

Wang

Hou

et al. 2023

J. Agric. Food Chem.

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“…This chemical inhibitor has been demonstrated to prevent a range of cytoskeletal remodeling events to prevent phagocytosis [276]. Cytochalasin D has been extensively used to study bacterial invasion [253,[276][277][278][279][280][281][282][283][284][285][286]. Importantly, while Cytochalasin D treatment inhibits invasion, it has not been shown to Fig.…”

Section: Elevated Rlu At 20 Min Post-infection Indicates a Defect In mentioning

confidence: 99%

Identification of host factors required for Yersinia pestis macrophage intracellular survival and their impact on vacuole maturation, acidification and trafficking.

Connor¹

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Ehrlichia's molecular tricks to manipulate their host cells

Moumène

Meyer

2016

Microbes and Infection

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Ehrlichia is a large genus of obligate intracellular Gram-negative bacteria transmitted by ticks that cause several emerging infectious diseases in humans and are pathogenic on rodents, ruminants, and dogs. Ehrlichia spp. invade and replicate either in endothelial cells, white blood cells, or within midgut cells and salivary glands of their vector ticks. In this review, we discuss the insights that functional studies are providing on how this group of bacteria exploits their host by subverting host innate immunity and hijacking cellular processes.

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The role of cytoskeleton, components of inositol phospholipid signaling pathway and iron in Ehrlichia canis in vitro proliferation

Cited by 5 publications

References 12 publications

Crucial Role of the Ca²⁺/CN Signaling Pathway in the Antifungal Activity of Seselin against Botrytis cinerea

Crucial Role of the Ca²⁺/CN Signaling Pathway in the Antifungal Activity of Seselin against Botrytis cinerea

Identification of host factors required for Yersinia pestis macrophage intracellular survival and their impact on vacuole maturation, acidification and trafficking.

Ehrlichia's molecular tricks to manipulate their host cells

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The role of cytoskeleton, components of inositol phospholipid signaling pathway and iron in Ehrlichia canis in vitro proliferation

Cited by 5 publications

References 12 publications

Crucial Role of the Ca2+/CN Signaling Pathway in the Antifungal Activity of Seselin against Botrytis cinerea

Crucial Role of the Ca2+/CN Signaling Pathway in the Antifungal Activity of Seselin against Botrytis cinerea

Identification of host factors required for Yersinia pestis macrophage intracellular survival and their impact on vacuole maturation, acidification and trafficking.

Ehrlichia's molecular tricks to manipulate their host cells

Contact Info

Product

Resources

About

Crucial Role of the Ca²⁺/CN Signaling Pathway in the Antifungal Activity of Seselin against Botrytis cinerea

Crucial Role of the Ca²⁺/CN Signaling Pathway in the Antifungal Activity of Seselin against Botrytis cinerea