The role of cytokinins in relation to flower-bud blasting in Iris cv. Ideal: Cytokinin determination by an improved enzyme-linked immunosorbent assay

Vonk, C. R.; Davelaar, E.; Ribot, S.A.

doi:10.1007/bf00025350

Cited by 50 publications

(23 citation statements)

References 16 publications

(25 reference statements)

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“…Purification, separation, and quantification of cytokinins were performed as described (Vonk et al, 1986;Jordi et al, 2000).…”

Section: Cytokinin Analysismentioning

confidence: 99%

Effects of PSAG12-IPT Gene Expression on Development and Senescence in Transgenic Lettuce

McCabe¹

2001

Plant Physiology

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An ipt gene under control of the senescence-specific SAG12 promoter from Arabidopsis (P SAG12 -IPT) significantly delayed developmental and postharvest leaf senescence in mature heads of transgenic lettuce (Lactuca sativa L. cv Evola) homozygous for the transgene. Apart from retardation of leaf senescence, mature, 60-d-old plants exhibited normal morphology with no significant differences in head diameter or fresh weight of leaves and roots. Induction of senescence by nitrogen starvation rapidly reduced total nitrogen, nitrate, and growth of transgenic and azygous (control) plants, but chlorophyll was retained in the lower (outer) leaves of transgenic plants. Harvested P SAG12 -IPT heads also retained chlorophyll in their lower leaves. During later development (bolting and preflowering) of transgenic plants, the decrease in chlorophyll, total protein, and Rubisco content in leaves was abolished, resulting in a uniform distribution of these components throughout the plants. Homozygous P SAG12 -IPT lettuce plants showed a slight delay in bolting (4-6 d), a severe delay in flowering (4-8 weeks), and premature senescence of their upper leaves. These changes correlated with significantly elevated concentrations of cytokinin and hexoses in the upper leaves of transgenic plants during later stages of development, implicating a relationship between cytokinin and hexose concentrations in senescence.Leaf senescence is a type of programmed cell death characterized by loss of chlorophyll, lipids, total protein, and RNA (Smart, 1994; Gan and Amasino, 1997). This process is believed to be an evolutionarily acquired, active genetic trait that contributes to plant fitness, for example, by remobilizing nutrients from vegetative tissues to reproductive organs (Oh et al., 1997). The biological importance and potential for improvement of crop characteristics, particularly plant productivity and postharvest storage, have prompted extensive physiological, molecular, and genetic analyses of leaf senescence (Nam, 1997). Senescence can be induced by environmental stress, such as low light intensity, nutrient deficiency, pathogen attack, drought, waterlogging, and detachment from the plant. Endogenous factors, including leaf age and reproductive development, also trigger senescence (Smart, 1994; Gan and Amasino, 1997).Plant growth regulators, including auxins, gibberellins, ethylene, abscisic acid, and cytokinins, are believed to play major roles in regulating senescence. Attention has focused on cytokinins that are key components of plant senescence (Van Staden et al., 1988;Singh et al., 1992;Gan and Amasino, 1996, 1997; Buchanan-Wollaston, 1997;Nam, 1997). These compounds have been implicated in several aspects of plant development and are thought to be synthesized mainly in the roots and transported to the shoots via the xylem (Gan and Amasino, 1996). Three main approaches have been used to study the effect of cytokinins in plant senecence, namely exogenous application of cytokinin solutions, measurement of endogenous cytokinins duri...

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“…Purification, separation, and quantification of cytokinins were performed as described (Vonk et al, 1986;Jordi et al, 2000).…”

Section: Cytokinin Analysismentioning

confidence: 99%

Effects of PSAG12-IPT Gene Expression on Development and Senescence in Transgenic Lettuce

McCabe¹

2001

Plant Physiology

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“…The extraction, purification and determination by enzyme immuno assay method (EIA) were performed as described by Kuiper et al (1989) and Vonk et al (1986).…”

Section: Cytokininsmentioning

confidence: 99%

Actual cytokinin concentrations in plant tissue as an indicator for salt resistance in cereals

1990

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Growth rates of roots and shoots of hydroponically grown plants of a salt resistant variety of barley decreased rapidly after an exposure to 65 M m -3 sodium chloride. This growth rate reduction tended to be temporarily and it was accompanied by a lowered shoot to root ratio. At the same time growth reduction took place, the cytokinin concentrations in root and shoot tissue decreased rapidly too, as they were measured by an enzyme-linked immuno assay. The addition of 5-10 9 Mm-3 benzyladenine (BA) retarded the decreases of growth rates, shoot to root ratio and internal cytokinin concentrations.Growth rates of roots and shoot of identically grown plants of salt sensitive varieties of barley maintained their growth rates and shoot to root ratio after an exposure to NaC1 for at least ten days in combination with unchanged levels of endogenous cytokinins. After ca 14 days NaC1 induced severe decreases in RGR values, but not in shoot to root ratio. Addition of benzyladenine inhibited growth to some extent and enhanced the cytokinin concentration.It was concluded, that cytokinin concentrations measured directly after exposure to NaC1 form a promising screening method for salt resistance. The addition of BA made salt resistance varieties behave as salt sensitive varieties.

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“…Enzyme-linked immunoassays (ELISA) were carried out by means of Costar microtitre plates (flat bottom, 96 wells), as described by Vonk et al (35). The following buffers were used: a coating buffer (0.05 M Na2C03-NaHCO,, pH 9.6), a PBST buffer, and a substrate buffer (coating buffer + 0.5 mM MgCl,).…”

Section: Enzyme-linked Immunoassaymentioning

confidence: 99%

Gynodioecy in Plantago lanceolata. VI. Functions of cytokinins in growth, development, and reproduction of two sex types

Olff¹,

Kuiper²,

Damme³

et al. 1989

Can. J. Bot.

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. 1989. Gynodioecy in Plantago lanceolata. VI. Functions of cytokinins in growth, development, and reproduction of two sex types. Can. J. Bot. 67: 2765-2769. Theoretical models predict that male sterile plants of gynodioecious species should show at least some compensation for their disadvantage of not reproducing as males through female component of fitness, In this study, growth, development, and reproduction of a hermaphrodite and a male sterile family of Plantago lanceolata L. were compared under controlled conditions. The male sterile plants produced more and longer spikes and had relatively longer styles. The male sterile plants achieved their final biomass sooner, by an earlier formation of side rosettes, and flowered earlier. The hypothesis was tested as to whether cytokinins in the plants are involved as a pleiotropic factor in either or both sex expression and the various plant characteristics associated with the male sterile phenotype. The roots of the male sterile plants had higher concentrations of putative zeatin riboside than the roots of the hermaphroditic plants, as quantified by an enzyme-linked immunoassy after separation of cytokinins by high performance liquid chromatography. Spraying the plants with benzyladenine did not affect internal cytokinin concentrations or sex expression. Benzyladenine spray increased the growth rate of the main rosette and stimulated floral initiation. Our results indicate that cytokinins are possibly involved in determining the morphological differences between sex types in this species.OLFF, H., KUIPER, D., VAN des cytokinines endogbnes sont impliquCes comme facteur plCiotrope dans l'expression de I'un ou des deux sexes et des diverses caractCristiques vCgCtales associCes au phCnotype i stCrilitC mile fut vCrifiCe. Les racines des plantes i stCrilitC mile avaient des concentrations plus ClevCes en zCatine riboside que les racines des plantes hermaphrodites, tel que quantifiC par un essai immunologique, aprks sCparation des cytokinines par la chromatographie liquide i haute performance. L'arrosage des plantes avec de la benzyladknine n'a pas affect6 les concentrations en cytokinines internes, ni l'expression du sexe. L'arrosage avec la benzyladknine a augment6 le taux de croissance de la rosette principale et stimulC l'initiation florale. Nos rksultats indiquent que les cytokinines sont possiblement impliquCes dans la determination des diffkrences morphologiques entre les types de sexe chez cette espbce.[Traduit par la revue]

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The role of cytokinins in relation to flower-bud blasting in Iris cv. Ideal: Cytokinin determination by an improved enzyme-linked immunosorbent assay

Cited by 50 publications

References 16 publications

Effects of PSAG12-IPT Gene Expression on Development and Senescence in Transgenic Lettuce

Effects of PSAG12-IPT Gene Expression on Development and Senescence in Transgenic Lettuce

Actual cytokinin concentrations in plant tissue as an indicator for salt resistance in cereals

Gynodioecy in Plantago lanceolata. VI. Functions of cytokinins in growth, development, and reproduction of two sex types

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