The role of cysteine in improving somatic embryos of salak Sidempuan <i>(Salacca sumatrana</i> Becc.)

Elimasni,; Haryati, Haryati; Nurwahyuni, Isnaini; Gusvani, V

doi:10.1088/1742-6596/1116/5/052019

Cited by 2 publications

(1 citation statement)

References 5 publications

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“…(Modeste et al, 2017)and Salacca sumatrana Becc. (Elimasni et al, 2020).The differential accumulation of phenolic compounds, in the culture medium of C. arabica could be a major cause of the interruption to the cell division pattern, affecting cellular proliferation and the process of somatic embryogenesis (Nic-Can et al, 2015).Thebalanced concentration and distribution of polyphenols induces embryogenesis (Gallego Rúa et al, 2016). Therefore, the reduced browning of coffee callus resulted from the secretion of phenolic compounds in scale 2 (callus with 1% to 33% of browning) affectedSE induction (Tables 2 and 3).…”

Section: Discussionmentioning

confidence: 99%

Effects of Reducing Browning on the Somatic Embryogenesis of Coffea arabica

2023

JJBS

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The browning of plant tissues is a major constraint in plant tissue culture.This is especially true for embryogenic cells grown on a culture medium to produce true-to-type hybrid coffee via somatic embryogenesis. There isvery little information in the current literature of the rate of browning intensity involved the development stage of coffee callus.We investigated the use ofthe antioxidantcysteine (Cys) and activated charcoal (AC) to induce callus formation, reduce browning of the callus, and induce somatic embryos of Coffea arabica.Explants were cultured on MS media supplemented with Cys and AC. The study involved varying the Cys rates in the order of 30, 35, and 40 mg/L, as well as varying the AC rates in the order of 0.008%, 0.01%, and 0.08% (w/v). The statistical analysis of the results was performed using the Statistical Package for Social Sciences (SPSS) version 14, with a significance level set at p<0.05. Neither anti-browningagent increased the rate of callus induction.The use ofMS+1 µM 2,4-dichlorophenoxyacetic acid (2,4-D) +5 µM 6-benzyl adenine (BA) media supplemented with 30 or 35 mg/LCys or 0.008% AC reduced the rate of tissue browning and induced a high rate of somatic embryo (SE) induction (80%, 84.21%, and 65%, respectively). SEstreated with30 mg/LCys couldbe germinated to the asynchronous developmental stages within six months.They couldbe converted to mature plantlets on MS without plant growth regulator hormones. Therefore, theuse ofCys and AC could be valuable for the propagation of Coffee arabicaL. cv. CatimorCIFC 7963.

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Section: Discussionmentioning

confidence: 99%

Effects of Reducing Browning on the Somatic Embryogenesis of Coffea arabica

2023

JJBS

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Cystathionine Gamma-Lyase Regulate Psilocybin Biosynthesis in Gymnopilus dilepis Mushroom via Amino Acid Metabolism Pathways

Yao

Wei

Lin

et al. 2022

JoF

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As a potential medicine for the treatment of depression, psilocybin has gradually attracted attention. To elucidate the molecular mechanism regulating psilocybin synthesis in Gymnopilus dilepis, ultra-performance liquid chromatography (UPLC) was used to detect the changes in psilocybin content after S-adenosyl-l-homocysteine (SAH) treatment and the changes of psilocybin content in different parts (stipe and pileus), and RNA-Seq was used to explore the mechanism of psilocybin content changes. In this study, the psilocybin content in G. dilepis mycelia treated with SAH was significantly lower than that in the control group, and the content of psilocybin in the stipe was significantly higher than that in the pileus. Transcriptome analysis revealed that differential expression genes (DEGs) were associated with cysteine and methionine metabolism. In particular, the transcription levels of genes encoding Cystathionine gamma-lyase (CTH) in different treatments and different parts were positively correlated with psilocybin content. In addition, we found that the exogenous addition of CTH activity inhibitor (DL-propargylglycine, PAG) could reduce the content of psilocybin and L-serine, and the content of psilocybin and L-serine returned to normal levels after L-cysteine supplementation, suggesting that psilocybin synthesis may be positively correlated with L-cysteine or CTH, and L-cysteine regulates the synthesis of psilocybin by affecting L-serine and 4-hydroxy-L-tryptophan. In conclusion, this study revealed a new molecular mechanism that affects psilocybin biosynthesis, which can provide a theoretical basis for improving psilocybin synthesis and the possibility for the development of biomedicine.

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The role of cysteine in improving somatic embryos of salak Sidempuan (Salacca sumatrana Becc.)

Cited by 2 publications

References 5 publications

Effects of Reducing Browning on the Somatic Embryogenesis of Coffea arabica

Effects of Reducing Browning on the Somatic Embryogenesis of Coffea arabica

Cystathionine Gamma-Lyase Regulate Psilocybin Biosynthesis in Gymnopilus dilepis Mushroom via Amino Acid Metabolism Pathways

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