The role of CTNNB1 and LEF1 in feather follicles development of Anser cygnoides and Anser anser

In ovo injection of CHIR-99021 promotes feather follicles development via activating Wnt/β-catenin signaling pathway during chick embryonic period

“…By E17, it was observed that feather follicles morphogenesis was mainly completed with feather fiber elongation, and then feather fiber continuously differentiated and matured into feather follicles ( Gong et al, 2018 ). Moreover, we found that β-catenin distributed in the processing of feather follicles morphogenesis at all stages ( Figure 2 ), which was similar to previous studies ( Sun et al, 2020 ). Therefore, the time nodes, in ovo injection of CHIR-99021 at E9 and observations at E17, were chosen to further understand the role of the Wnt/β-catenin in the feather follicles morphogenesis during chick embryonic period.…”

“…LEF1 is an important downstream target regulating transcription and translation of the Wnt signaling pathway. In addition, the transcription factor LEF1 is a member of the Lef/Tcf family that is necessary for the differentiation of Hair Follicle Stem Cells ( HFSCs ), and the formation of Inner Root Sheath in feather follicles ( Brunner et al, 2017 ; Sun et al, 2020 ). Thus, we also investigated the expression of LEF1 at gene and protein levels.…”

Lef1 and Dlx3 May Facilitate the Maturation of Secondary Hair Follicles in the Skin of Gansu Alpine Merino

“…In contrast, in this study, we observed that the peak mRNA and protein levels of Lef1 and Dlx3 occurred at the 30th and 60th days, respectively; however, both peaks occurred during the period of fastest maturation of the secondary hair follicles. Furthermore, in a study of spatiotemporal expression patterns, it was observed that Lef1 was an important regulatory gene in the Wnt pathway for the induction of primary feather follicles and a positive regulator of primary feather follicle development in goose skin [ 31 ], while Lef1 also had an effect on the growth and development of swan and gosling hair follicles [ 32 ]. In contrast, a weighted gene co-expression network analysis (WGCNA) was applied in velvet goats to identify Lef1 as a key factor in hair follicle cycle development, and it was further determined to play an important role in hair follicle cycle development using RT-qPCR [ 33 ].…”

Dermal FOXO3 activity in response to Wnt/β-catenin signaling is required for feather follicle development of goose embryos (Anser cygnoides)

“…Wnt/β-catenin serves as a conserved classical pathway signaling widely active in feather follicles, which modulates epithelial-mesenchymal cell interactions and influences the embryogenesis of the feather follicle ( Yue et al, 2006 ). Lymphatic enhancer factor 1 ( LEF1 ), a transcription factor, is one of the main downstream members of the positively regulated Wnt/β-catenin signaling pathway ( Ota et al, 2016 ; Xie et al, 2020a ; Sun et al, 2020 ). In addition, Lef1 regulates cell differentiation and tissue self-renewal by binding to other transcription factors to form complexes that activate target transcription genes ( Zhang et al, 2013 ).…”