The role of copper in particulate methane monooxygenase from Methylosinus trichosporium OB3b

Takeguchi, Masayuki; Miyakawa, Kiyoko; Okura, Ichiro

doi:10.1016/s1381-1169(98)00123-x

Cited by 37 publications

(58 citation statements)

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“…The results also suggest that Cu-mb is the probable source of the variability, complexity and controversy associated with the copper centres of the pMMO (Basu et al, 2003;Chan et al, 2004;Choi et al, 2003;Lieberman et al, 2003;Lieberman & Rosenzweig, 2004Nguyen et al, 1994Nguyen et al, , 1996Nguyen et al, , 1998Takeguchi & Okura, 2000;Takeguchi et al, 1999;Téllez et al, 1998;Yuan et al, 1997Yuan et al, , 1998aYuan et al, , b, 1999Zahn & DiSpirito, 1996). Laboratories examining the pMMO have differed in the analysis of the EPR spectra.…”

Section: Discussionmentioning

confidence: 88%

Effect of methanobactin on the activity and electron paramagnetic resonance spectra of the membrane-associated methane monooxygenase in Methylococcus capsulatus Bath

et al. 2005

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Improvements in the purification of methanobactin (mb) from either Methylosinus trichosporium OB3bT or Methylococcus capsulatus Bath resulted in preparations that stimulated methane-oxidation activity in both whole-cell and cell-free fractions of Methylococcus capsulatus Bath expressing the membrane-associated methane monooxygenase (pMMO). By using washed membrane factions with pMMO activities in the 290 nmol propylene oxidized min "1 (mg protein) "1 range, activities approaching 400 nmol propylene oxidized min "1 (mg protein) "1 were commonly observed following addition of copper-containing mb (Cu-mb), which represented 50-75 % of the total whole-cell activity.The stimulation of methane-oxidation activity by Cu-mb was similar to or greater than that observed with equimolar concentrations of Cu(II), without the inhibitory effects observed with high copper concentrations. Stimulation of pMMO activity was not observed with copper-free mb, nor was it observed when the copper-to-mb ratio was <0?5 Cu atoms per mb. The electron paramagnetic resonance (EPR) spectra of mb differed depending on the copper-to-mb ratio. At copper-to-mb ratios of <0?4 Cu(II) per mb, Cu(II) addition to mb showed an initial coordination by both sulfur and nitrogen, followed by reduction to Cu(I) in <2 min. At Cu(II)-to-mb ratios between 0?4 and 0?9 Cu(II) per mb, the intensity of the Cu(II) signal in EPR spectra was more representative of the Cu(II) added and indicated more nitrogen coordination. The EPR spectral properties of mb and pMMO were also examined in the washed membrane fraction following the addition of Cu(II), mb and Cu-mb in the presence or absence of reductants (NADH or duroquinol) and substrates (CH 4 and/or O 2 ). The results indicated that Cu-mb increased electron flow to the pMMO, increased the free radical formed following the addition of O 2 and decreased the residual free radical following the addition of O 2 plus CH 4 . The increase in pMMO activity and EPR spectral changes to the pMMO following Cu-mb addition represent the first positive evidence of interactions between the pMMO and Cu-mb.

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Section: Discussionmentioning

confidence: 88%

Effect of methanobactin on the activity and electron paramagnetic resonance spectra of the membrane-associated methane monooxygenase in Methylococcus capsulatus Bath

et al. 2005

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“…Third, previous studies have shown that zinc inhibits pMMO activity in M. capsulatus (Bath) and M. trichosporium OB3b membrane extracts (15)(16)(17). Up to seven zinc ions have been suggested to bind to M. capsulatus (Bath) pMMO and have been proposed to inhibit by either replacing active site copper ions or another copper site that is involved in reducing the active site (15)(16)(17). Based on the crystal structures, which were determined after these studies, it is also possible that inhibition is due to zinc occupation of the pmoC intramembrane metal-binding site.…”

mentioning

confidence: 98%

“…Second, the best enzymatic activity is obtained from intact pMMO containing all three subunits, suggesting that the transmembrane regions have an important function (11). Third, previous studies have shown that zinc inhibits pMMO activity in M. capsulatus (Bath) and M. trichosporium OB3b membrane extracts (15)(16)(17). Up to seven zinc ions have been suggested to bind to M. capsulatus (Bath) pMMO and have been proposed to inhibit by either replacing active site copper ions or another copper site that is involved in reducing the active site (15)(16)(17).…”

mentioning

confidence: 99%

Effects of Zinc on Particulate Methane Monooxygenase Activity and Structure

Sirajuddin

Barupala

Helling

et al. 2014

Journal of Biological Chemistry

173

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Background: Particulate methane monooxygenase (pMMO) is a membrane-bound metalloenzyme that oxidizes methane to methanol. Results: Metal binding data and crystal structures reveal that zinc inhibits pMMO at two sites. Conclusion: Zinc does not inhibit pMMO by binding at the active site but may hinder another function such as proton transfer. Significance: New insight into the function of an environmentally and industrially important enzyme has been obtained.

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“…The MMO oxidizes methane to methanol and MDH catalyzes the oxidation of methanol to formaldehyde. Methylosinus trichosporium OB3b is a methanotrophic bacterium and contains two forms of MMOs: a soluble (sMMO) and a membrane-bound particulate (pMMO) whose syntheses depend on growth conditions (Murrell et al, 2000;Nielsen et al, 1997;Takeguchi and Okura, 2000).…”

Section: Introductionmentioning

confidence: 99%

A Review on Biogas Interception Processes in Municipal Landfill

Qasaimeh¹,

Abdallah-Qasaimeh²,

Hani³

2015

J. of Environmental Science and Technology

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Biogas in landfill is being captured by natural and engineered processes. The natural processes are represented by biological activities such as bacterial methane oxidation and plant uptake for carbon dioxide at topsoil layer. Landfill gas is transported through soil layers in landfill top or in nearby areas before being released to the atmosphere. Whilst transported in the soil layers the biogas is mixed with atmospheric air and the methane may hence be oxidized by the methanotrophic bacteria in the soil using oxygen from atmosphere. Methane oxidation is affected by different environmental factors such as; temperature, water content, nutrients, substrate and oxygen concentrations. One of the ways to decrease greenhouse emissions in the future is to plant fast growing woody crops thereby sequestering carbon and displacing fossil fuels by harvesting woody biomass for bio-energy, or by storing carbon in long-lived woody products. Plant uptake for carbon dioxide is affected by some parameters such as; CO 2 concentration, nitrogen concentration, water content and temperature. The engineered processes are represented by various physical biogas extractions; gas is collected using network of collection pipes and wells. The gas collection efficiency in landfills is between 40-90%. Landfill gas can be collected by either a passive or an active collection system. Passive gas collection systems use existing variations in landfill pressure and gas concentrations to vent landfill gas into the atmosphere or a control system. Active gas collection is considered a good means of landfill gas collection. An active collection system composed of extraction wells connected to header pipe to a pump that delivers gas for energy recovery.

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The role of copper in particulate methane monooxygenase from Methylosinus trichosporium OB3b

Cited by 37 publications

References 10 publications

Effect of methanobactin on the activity and electron paramagnetic resonance spectra of the membrane-associated methane monooxygenase in Methylococcus capsulatus Bath

Effect of methanobactin on the activity and electron paramagnetic resonance spectra of the membrane-associated methane monooxygenase in Methylococcus capsulatus Bath

Effects of Zinc on Particulate Methane Monooxygenase Activity and Structure

A Review on Biogas Interception Processes in Municipal Landfill

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