The role of concentration on drop formation and breakup of collagen, fibrinogen, and thrombin solutions during inkjet bioprinting

Gudapati, Hemanth; Özbolat, İbrahim T.

doi:10.1101/2020.10.06.328187

Cited by 2 publications

(2 citation statements)

References 55 publications

(76 reference statements)

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“…Even though they are not visible by UV spectrometry, the microfibers of collagen might already be present in the core solution and perturb the coflow like that for the inkjet bioprinting case. 34 Since jet fragmentation is linked to the capillary instability, where perturbations are exponentially amplified, monitoring jet features is a more sensitive way to evaluate the state of collagen dispersion.…”

Section: Resultsmentioning

confidence: 99%

Encapsulation of Cells in a Collagen Matrix Surrounded by an Alginate Hydrogel Shell for 3D Cell Culture

Bouhlel

Kui

Bibette

et al. 2022

ACS Biomater. Sci. Eng.

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Numerous techniques for mammalian cell culture have been developed to mimic the complex in vivo three-dimensional structure of tissues and organs. Among them, the sole use of proteins to create a matrix where cells are embedded already gives rise to self-organized multicellular assemblies. Loading cells in a controlled extracellular matrix along with cell culture and monitoring through a strategy that is compatible with pipetting tools would be beneficial for high throughput screening applications or simply for a standardized method. Here, we design submillimeter compartments having a thin alginate hydrogel shell and a core made of a collagen matrix where cells are embedded. The process, using a microfluidic device, is based on a high speed co-extrusion in air, leading to a compound jet whose fragmentation is controlled. The resulting core–shell liquid drops are then collected in a gelling bath that triggers a fast hardening of the shell and is followed by a slower self-assembly of collagen molecules into fibers. We show how to formulate the core solution in order to maintain cell viability at physiological conditions that otherwise induce tropocollagen molecules to self-assemble, while being able to prevent flow disturbances that are detrimental for this jetting method. Encapsulated Caco-2 cells, mainly used to model the intestinal barrier, proliferate and form a closed polarized epithelial cell monolayer where the apical membrane faces the continuous medium.

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Section: Resultsmentioning

confidence: 99%

Encapsulation of Cells in a Collagen Matrix Surrounded by an Alginate Hydrogel Shell for 3D Cell Culture

Bouhlel

Kui

Bibette

et al. 2022

ACS Biomater. Sci. Eng.

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“…2 The advantages of this deposition technique are low material consumption, low waste, and no mask needed, and it is a noncontact technique. 3 Popularity and utilization of this technique can be demonstrated by its application fields including printed electronics (e.g., temperature, 4 gas, 5 and humidity 6 sensors; wearable monitoring devices; 7 light-emitting diodes; 8 microsupercapacitors, 9 solar cells, 10,11 and others) and bioapplications such as a study of enzyme activity in biosensor fabrication; 12 polymer artificial muscles; 13 printing of collagen, fibrinogen, and thrombin; 14 and printing of biological materials such as cells and antibiotics. 1 Other applications include, for example, threedimensional (3D) prototyping, patterning, and coating.…”

Section: ■ Introductionmentioning

confidence: 99%

Inkjet Printability Assessment of Weakly Viscoelastic Fluid: A Semidilute Polyvinylpyrrolidone Solution Ink Case Study

et al. 2021

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Here, we present an integrated approach to the weakly viscoelastic fluid printability assessment by using global dimensionless criteria (DC). The problem was studied on a model semidiluted polyvinylpyrrolidone water-based ink. For the study purpose, the ink composition was kept as simple as possible. First, the solution density, viscosity, and surface tension were determined. Obtained data were used for testing limitations of DC printability diagrams already available for Newtonian fluids. A replotted version of the original Kim and Baek’s map was developed emphasizing the importance of surface tension in the drop formation process. Another set of DC (e.g., Ec and De) was also used for a real evaluation of the viscoelasticity effect on both jetting conditions and drop formation. The polymer relaxation time as a crucial parameter for viscoelasticity was shown to be calculated using the Kuhn segment length rather than from Zimm and Rouse theories for diluted polymer systems. Then, a two-dimensional diagram using four DC (Oh and De with Ec and El as parameters) is proposed based on the famous McKinley’s work. The diagram describes the interplay of possible forces responsible for filament thinning and breakup processes. Obtained results were supported by further experiments involving drop ejection and formation, determination of critical polymer concentration, and others. The proposed diagram promises a useful initial step in further investigations of viscoelasticity of polymer compounds by inkjet printing.

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The role of concentration on drop formation and breakup of collagen, fibrinogen, and thrombin solutions during inkjet bioprinting

Cited by 2 publications

References 55 publications

Encapsulation of Cells in a Collagen Matrix Surrounded by an Alginate Hydrogel Shell for 3D Cell Culture

Encapsulation of Cells in a Collagen Matrix Surrounded by an Alginate Hydrogel Shell for 3D Cell Culture

Inkjet Printability Assessment of Weakly Viscoelastic Fluid: A Semidilute Polyvinylpyrrolidone Solution Ink Case Study

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