The role of Cl− in pollen germination and tube growth

Breygina, Maria; Матвеева, Н. П.; Ермаков, И. П.

doi:10.1134/s1062360409030047

Cited by 17 publications

(19 citation statements)

References 32 publications

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“…It has been established that pollen tube germination does not require extracellular anions (Weisenseel & Jaffe, 1976). Nevertheless, our results, combined with those of Zonia et al (2002) and Breygina et al (2009a), suggest that pollen grains may have considerable anionic reserves, which are readily available for transport across the membrane. If this is the case, the channels responsible for the currents we observed could be involved in the exit of anions during grain hydration and tube growth processes.…”

Section: New Phytologistmentioning

confidence: 50%

“…, 2002; Breygina et al. , 2009a) and Cl − release from tobacco pollen grain (Breygina et al. , 2009a), this inhibitor was then tested on the remaining anionic current.…”

Section: Resultsmentioning

confidence: 99%

“…After complete rundown, a stable level of current was achieved. As it is known that NPPB, a known inhibitor of anion channels, blocks tobacco and lily pollen tube growth (Zonia et al, 2002;Breygina et al, 2009a) and Cl ) release from tobacco pollen grain (Breygina et al, 2009a), this inhibitor was then tested on the remaining anionic current. The application of NPPB allowed us to discriminate between two additional populations of anionic currents, one that was insensitive to the inhibitor (I Cl2 ) and one that was completely abolished in the presence of the inhibitor (I Cl3 ).…”

Section: Resultsmentioning

confidence: 99%

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Calcium‐regulated anion channels in the plasma membrane of Lilium longiflorum pollen protoplasts

et al. 2011

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Summary• Currents through anion channels in the plasma membrane of Lilium longiflorum pollen grain protoplasts were studied under conditions of symmetrical anionic concentrations by means of patch-clamp whole-cell configuration.• With Cl ) -based intra-and extracellular solutions, three outward-rectifying anion conductances, I Cl1 , I Cl2 and I Cl3 , were identified. These three activities were discriminated by differential rundown behaviour and sensitivity to 5-nitro-2-(phenylpropylamino)-benzoate (NPPB), which could not be attributed to one or more channel types. All shared strong outward rectification, activated instantaneously and displayed a slow time-dependent activation for positive potentials. All showed modulation by intracellular calcium ([Ca 2+ ] in ), increasing intensity from 6.04 nM up to 0.5 mM (I Cl1 ), or reaching a maximum value with 8.50 lM (I Cl2 and I Cl3 ).• After rundown, the anionic currents measured using NO 3 ) -based solutions were indistinguishable, indicating that the permeabilities of the channels for Cl ) and NO 3 ) are similar. Additionally, unitary anionic currents were measured from outside-out excised patches, confirming the presence of individual anionic channels.• This study shows for the first time the presence of a large anionic conductance across the membrane of pollen protoplasts, resulting from the presence of Ca 2+ -regulated channels. A similar conductance was also found in germinated pollen. We hypothesize that these putative channels may be responsible for the large anionic fluxes previously detected by means of self-referencing vibrating probes.

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Section: New Phytologistmentioning

confidence: 50%

“…, 2002; Breygina et al. , 2009a) and Cl − release from tobacco pollen grain (Breygina et al. , 2009a), this inhibitor was then tested on the remaining anionic current.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Calcium‐regulated anion channels in the plasma membrane of Lilium longiflorum pollen protoplasts

et al. 2011

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“…2). High Cl -levels blocked tobacco pollen tube growth, but did not immediately block smooth vesicle endocytosis at the apex (Breygina et al, 2009), suggesting that this endosomal pathway differs from the 'kiss-and-run' exocytosis/endocytosis pathway that has been suggested to occur at the pollen tube apex (Samaj et al, 2006;Bove et al, 2008).…”

Section: Ion Fluxes In Pollen Tube Growthmentioning

confidence: 92%

“…Anion efflux from the apex occurs in phase with growth. Disruption of Ca 2+ or Cl -fluxes leads to apical swelling and growth arrest (Holdaway-Clarke and Hepler, 2003;Breygina et al, 2009). The new model considers that ion fluxes in pollen tubes are involved in regulating and responding to changes in cell hydrodynamics in the apical region, and are essential elements in regulatory volume decrease and regulatory volume increase Munnik, 2007, 2009).…”

Section: Ion Fluxes In Pollen Tube Growthmentioning

confidence: 99%

Spatial and temporal integration of signalling networks regulating pollen tube growth

Zonia

2010

Journal of Experimental Botany

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The overall function of a cell is determined by its contingent of active signal transduction cascades interacting on multiple levels with metabolic pathways, cytoskeletal organization, and regulation of gene expression. Much work has been devoted to analysis of individual signalling cascades interacting with unique cellular targets. However, little is known about how cells integrate information across hierarchical signalling networks. Recent work on pollen tube growth indicates that several key signalling cascades respond to changes in cell hydrodynamics and apical volume. Combined with known effects on cytoarchitecture and signalling from other cell systems, hydrodynamics has the potential to integrate and synchronize the function of the broader signalling network in pollen tubes. This review will explore recent work on cell hydrodynamics in a variety of systems including pollen, and discuss hydrodynamic regulation of cell signalling and function including exocytosis and endocytosis, actin cytoskeleton reorganization, cell wall deposition and assembly, phospholipid and inositol polyphosphate signalling, ion flux, small G-proteins, fertilization, and self-incompatibility. The combined data support a newly emerging model of pollen tube growth.

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Oxygen radicals and cytoplasm zoning in growing lily pollen tubes

et al. 2021

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Recently redox-regulation of tip growth has been extensively studied, but differential sensitivity of growing cells to particular ROS and their subcellular localization is still unclear.Here we used specific dyes to provide mapping of H 2 O 2 and O • 2 ¯ in short and long pollen tubes. We found apical accumulation of H 2 O 2 and H 2 O 2 -producing organelles in the shank that were not colocalized with O • 2 ¯-producing mitochondria. Differential modulation of ROS content of the germination medium affected both growth speed and pollen tube morphology. Oxygen radicals affected ionic zoning: membrane potential and pH gradients. OH• caused depolarization all along the tube while O • 2 ¯ provoked hyperpolarization and cytoplasm alkalinization. O • 2 ¯ accelerated growth and reduced tube diameter, indicating that this ROS can be considered as pollen tube growth stimulator along with H 2 O 2 . Serious structural disturbances were observed upon exposure to OH• and H 2 O 2 and O • 2 ¯ quencher MnTMPP: pollen tube growth slowed down and ballooned tips formed in both cases, but in the presence of OH• membrane transport and organelle distribution was affected as well. OH•, thus, can be considered as a negative influence on pollen tubes which, presumably, have mechanisms for leveling it. The assumption was confirmed by EPR spectroscopy: pollen tubes actively reduce OH• content in the incubation medium.Recent studies of pollen tube growth have been attributed to internal and external signals affecting cytoplasm structural and functional as well as non-uniformly distributed ion currents. A number of data indicate the great importance of redox-regulation: ROS are produced on stigmas of various plants species during the pollination period

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The role of Cl− in pollen germination and tube growth

Cited by 17 publications

References 32 publications

Calcium‐regulated anion channels in the plasma membrane of Lilium longiflorum pollen protoplasts

Calcium‐regulated anion channels in the plasma membrane of Lilium longiflorum pollen protoplasts

Spatial and temporal integration of signalling networks regulating pollen tube growth

Oxygen radicals and cytoplasm zoning in growing lily pollen tubes

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