“…Therefore, the analysis of CTCs holds great promise for several clinical utilizations (e.g., cancer detection [4,5], prognosis evaluation of cancer [6,7], therapeutic response evaluation of cancer [8][9][10], or monitoring of cancer metastasis [11,12]). Compared with the clinical applications of CTCs simply based on the quantification of their cell number [6,11], the analysis of CTCs at molecular level (e.g., gene expression analysis) could pave a more promising route to Micromachines 2023, 14, 2170 2 of 17 comprehensively understand CTC heterogeneity [13,14], cancer progression [15,16], or mechanisms of therapeutic resistance [17,18]. To achieve this goal, the harvest of highpurity and all possible CTCs with different subtypes from the blood samples of cancer patients is crucially important.…”