The role of CD178+ mononuclear cells in the development of threatened late abortion in women with first-trimester threatened pregnancy interruption and a history of recurrent miscarriage

Batrak, Batrak N.V.; Малышкина, А. И.; Sotnikova, Sotnikova N.Yu.; Kroshkina, Kroshkina N.V.

doi:10.18565/aig.2020.5.70-77

Cited by 4 publications

(1 citation statement)

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“…Currently, the attention is attracted by the immune mechanisms of recurrent pregnancy loss. Recurrent miscarriage in 50% cases is associated with pathological changes in various arms of the immune system and an inadequate maternal response to a semi-allogeneic fetus [2]. Pregnancy is a complex multi-stage process that includes fertilization, implantation, placentation and further embryonic development.…”

Section: Abstract: Pregnancy Threatened Abortion Recurrent Miscarriage B-lymphocytes Baff Aprilmentioning

confidence: 99%

Regulation of Peripheral B-Lymphocyte Differentiation in Recurrent Miscarriage

et al. 2021

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The important role of immune disorders in recurrent miscarriage has been proven. Clarification of the character of B-lymphocyte differentiation and its regulation factors in women with threatened miscarriage and recurrent miscarriage in history is an urgent problem, since it will reveal the immune mechanisms of the pathogenesis of this pathology. Purpose: to establish the features of B-lymphocyte differentiation and factors of its regulation in women with a history of recurrent miscarriage and threatening spontaneous miscarriage at the time of examination.Were examined pregnant women aged 18-40 years at a gestation period of 5-12 weeks. The main group consisted of 60 pregnant women with a threatening spontaneous miscarriage at the time of examination and a history of recurrent miscarriage. As a control, 35 pregnant women with uncomplicated pregnancy were examined. The comparison group consisted of 25 primary pregnant women with threatened spontaneous miscarriage at the time of examination. The material for the study was peripheral venous blood. Subpopulations of B-lymphocytes CD19+, CD19+ IgD+, CD20+IgM+, CD20+IgG+ were determined by flow cytometry; CD19+CD20- CD38+, CD19+CD27- , CD19+CD27+. Serum levels of BAFF and APRIL were assessed by enzyme-linked immunosorbent assay.In the main group, an increase in the proportion of B-cells, CD20+IgM+-lymphocytes and memory cells was recorded in the peripheral blood, along with a decrease in the level of naive cells and plasma cells. In the comparison group, an increase in the proportion of immature IgM+B-cells, circulating memory cells, along with a decrease in naive B-lymphocytes, was registered. in the main group there was a pronounced decrease in the serum BAFF level compared with the control and comparison groups. Analysis of the APRIL content showed a pronounced downward trend in groups with threatened miscarriage relative to healthy pregnant women. Thus, threatening habitual and sporadic miscarriages were associated with a shift in the differentiation of B-lymphocytes towards immature forms and a lack of regulatory influence of BAFF and APRIL, which is reflected in the disruption of B-cell homeostasis and weakening of humoral effector mechanisms at the systemic level. The revealed changes may indicate a single mechanism for the development of a threatening spontaneous miscarriage, the severity of which increases with repeated loss of pregnancy. These changes can lead to an increase in effector cytotoxic mechanisms and an increase in proinflammatory cytokines, which can lead to the development of damaging reactions in the fetoplacental complex, which can be reflected in the clinical picture of the threat of termination of pregnancy.

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Section: Abstract: Pregnancy Threatened Abortion Recurrent Miscarriage B-lymphocytes Baff Aprilmentioning

confidence: 99%

Regulation of Peripheral B-Lymphocyte Differentiation in Recurrent Miscarriage

et al. 2021

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Placental insufficiency in women with threatened early miscarriage and recurrent miscarriage in anamnesis

Batrak¹,

Малышкина²,

Sotnikova³

et al. 2022

Probl. reprod.

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Regulation of the functional activity of peripheral B lymphocytes in pregnant women with a history of recurrent miscarriage

et al. 2021

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BACKGROUND:The frequency of recurrent miscarriage is up to 5 % of all desired pregnancies and is mainly due to immunological disorders. Dysfunction in the regulation of the functional activity of B lymphocytes is the pathogenetic link in multiple obstetric complications, including habitual miscarriage. AIM:The aim of this study was to characterize the regulation of the functional activity of peripheral B lymphocytes in pregnant women with threatened spontaneous miscarriage and a history of habitual miscarriage. MATERIALS AND METHODS:We examined 88 women aged 18-40 years at a gestation period of 5-12 weeks. The main group consisted of 36 patients with threatened spontaneous miscarriage at the time of examination and a history of habitual miscarriage. The control group included 28 women with uncomplicated pregnancy. The comparison group consisted of 24 primary pregnant patients with threatened spontaneous abortion at the time of examination. BAFF and APRIL levels in the blood serum were determined by enzyme immunoassay. The content of CD19+BAFFR+B lymphocytes in the lymphocyte gate was evaluated in the peripheral blood by flow cytometry using monoclonal antibodies. Akt mRNA expression was assessed using real-time reverse-transcription quantitative polymerase chain reaction. CD19+В lymphocytes were isolated by direct magnetic separation. RESULTS:In the main group, there was an increase in expression of BAFF receptors on peripheral CD19+B lymphocytes and a decrease in the serum BAFF concentration compared to the parameters in the other study groups. We also found a pronounced trend towards a decrease in the serum APRIL level in the main and comparison groups of patients compared to healthy pregnant women. Besides, Akt mRNA expression in peripheral CD19+B lymphocytes was increased in the main group. CONCLUSIONS:Threatened habitual abortion is associated with the deficit of the regulatory influence of BAFF and APRIL, which is expressed in the disruption of B cell homeostasis and the weakening of humoral effector mechanisms.

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The role of CD178+ mononuclear cells in the development of threatened late abortion in women with first-trimester threatened pregnancy interruption and a history of recurrent miscarriage

Cited by 4 publications

References 0 publications

Regulation of Peripheral B-Lymphocyte Differentiation in Recurrent Miscarriage

Regulation of Peripheral B-Lymphocyte Differentiation in Recurrent Miscarriage

Placental insufficiency in women with threatened early miscarriage and recurrent miscarriage in anamnesis

Regulation of the functional activity of peripheral B lymphocytes in pregnant women with a history of recurrent miscarriage

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