“…Ketamine (100 mg/kg) and xylazine (10 mg/kg) were used to anesthetize the rat, and then the animal was placed in a stereotaxic apparatus (Stoelting, Wood Dale, U.S.A.) with incisor bar positioned at −3.3 mm and ear bars positioned symmetrically. Performing a routine surgery for intracerebroventricular microinjections in rats (Parsania, Moradi, Fatahi, & Haghparast, 2016; Zarrabian, Nasehi, Farrahizadeh, & Zarrindast, 2017), two stainless steel guide cannulas (9 mm length, 23 gauge) were bilaterally implanted 1 mm above the hind portion of the DG at: anterior-posterior (AP) = −3.8 mm, medial-lateral (ML) = ±1.3 mm, and dorsal-ventral (DV) = 3.4 mm ventral from the skull surface (Paxinos & Watson, 2007). A stylet was introduced into each guide cannula to prevent possible obstruction.…”