The role of bone marrow-derived cells in bone fracture repair in a green fluorescent protein chimeric mouse model

Taguchi, Kazuhiro; Ogawa, Rei; Migita, Makoto; Hanawa, Hideki; Ito, Hiromoto; Orimo, Hideo

doi:10.1016/j.bbrc.2005.03.119

Cited by 98 publications

(88 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This necrotic process triggers an immediate inflammatory response, bringing inflammatory cells, leucocytes and macrophages to the region. These are followed by the invasion of fibroblasts, mesenchymal stem cells and endothelial cells (Taguchi et al 2005). Growth factors and cytokines, important regulators of the healing process, are produced by the cells present in the regeneration area as well as released into this area from the surrounding tissues (damaged bone ends, muscles, periosteum and marrow; Gerstenfeld et al 2003;Malizos & Papatheodorou 2005).…”

Section: Bone Regeneration (A ) Biology Of Bone Regenerationmentioning

confidence: 99%

In silicobiology of bone modelling and remodelling: regeneration

Geris

Sloten

Oosterwyck

2009

Phil. Trans. R. Soc. A.

View full text Add to dashboard Cite

Bone regeneration is the process whereby bone is able to (scarlessly) repair itself from trauma, such as fractures or implant placement. Despite extensive experimental research, many of the mechanisms involved still remain to be elucidated. Over the last decade, many mathematical models have been established to investigate the regeneration process in silico. The first models considered only the influence of the mechanical environment as a regulator of the healing process. These models were followed by the development of bioregulatory models where mechanics was neglected and regeneration was regulated only by biological stimuli such as growth factors. The most recent mathematical models couple the influences of both biological and mechanical stimuli. Examples are given to illustrate the added value of mathematical regeneration research, specifically in the in silico design of treatment strategies for non-unions. Drawbacks of the current continuum-type models, together with possible solutions in extending the models towards other time and length scales are discussed. Finally, the demands for dedicated and more quantitative experimental research are presented.

show abstract

Section: Bone Regeneration (A ) Biology Of Bone Regenerationmentioning

confidence: 99%

In silicobiology of bone modelling and remodelling: regeneration

Geris

Sloten

Oosterwyck

2009

Phil. Trans. R. Soc. A.

View full text Add to dashboard Cite

show abstract

“…Thus, cells of the blood and bone marrow, such as immune cells, erythrocytes, and stem cells, ingress and are disrupted from the oxygen and nutrient supply at the injury site. This process leads to local tissue hypoxia and an inflammatory response, which is a result of migration of inflammatory cells, leukocytes, and macrophages into the fracture gap, triggering the formation of granulation tissue [43].…”

Section: Introductionmentioning

confidence: 99%

Human Early Fracture Hematoma Is Characterized by Inflammation and Hypoxia

Kolar

Gaber

Perka

et al. 2011

Clinical Orthopaedics &Amp; Related Research

165

181

View full text Add to dashboard Cite

show abstract

“…This triggers the invasion of inflammatory cells, macrophages and leukocytes, marking the start of the inflammatory phase. Simultaneously, growth factors and cytokines produced by the cells in the hematoma and surrounding tissues attract fibroblasts, mesenchymal stem cells (MSCs) and endothelial cells to the trauma site (Taguchi et al, 2005). The fracture callus fills with granulation tissue, forming the soft callus, in which the MSCs start to differentiate.…”

Section: Normal Fracture Healingmentioning

confidence: 99%

Oxygen as a critical determinant of bone fracture healing—A multiscale model

Carlier

Geris

Gastel

et al. 2015

Journal of Theoretical Biology

View full text Add to dashboard Cite

Highlights The influence of oxygen was incorporated in a multiscale model of fracture healing.  The results of the oxygen model were compared with experimental observations.  An extensive sensitivity analysis of the oxygen model indicated its robustness.  Adequate spatiotemporal oxygen patterns appear to be critical for bone healing. Keywordsoxygen -angiogenesis -fracture healing -multiscale model -non-union AbstractA timely restoration of the ruptured blood vessel network in order to deliver oxygen and nutrients to the fracture zone is crucial for successful bone healing. Indeed, oxygen plays a key role in the aerobic metabolism of cells, in the activity of a myriad of enzymes as well as in the regulation of several (angiogenic) genes. In this paper, a previously developed model of bone fracture healing is further improved with a detailed description of the influence of oxygen on various cellular processes that occur during bone fracture healing. Oxygen ranges of the cell-specific oxygen-dependent processes were established based on the state-of-the art experimental knowledge through a rigorous literature study. The newly developed oxygen model is compared with previously published experimental and in silico results. An extensive sensitivity analysis was also performed on the newly introduced oxygen thresholds, indicating the robustness of the oxygen model. Finally, the oxygen model was applied to the challenging clinical case of a critical sized defect (3 mm) where it predicted the formation of a fracture non-union. Further model analyses showed that the harsh hypoxic conditions in the central region of the callus resulted in cell death and disrupted bone healing thereby indicating the importance of a timely vascularization for the successful healing of a large bone defect. In conclusion, this work demonstrates that the oxygen model 3 is a powerful tool to further unravel the complex spatiotemporal interplay of oxygen delivery, diffusion and consumption with the several healing steps, each occurring at distinct, optimal oxygen tensions during the bone repair process.

show abstract

The role of bone marrow-derived cells in bone fracture repair in a green fluorescent protein chimeric mouse model

Cited by 98 publications

References 16 publications

In silicobiology of bone modelling and remodelling: regeneration

In silicobiology of bone modelling and remodelling: regeneration

Human Early Fracture Hematoma Is Characterized by Inflammation and Hypoxia

Oxygen as a critical determinant of bone fracture healing—A multiscale model

Contact Info

Product

Resources

About