The Role of Bone Marrow Derived Mesenchymal Stem Cells in Attenuation of Renal Failure in Adult Male Albino Rats

Ghanayem, Yousri El–Amir Ahmad; Ashour, Fawzy A; Rashed, Laila A.; Zoair, Mohammad Abul Hasan; Hai, Osama Mohammad Abd El –

doi:10.12816/0026279

Cited by 1 publication

(1 citation statement)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BMMSCs were observed under a Biobase inverted microscope to con rm the characteristics of adherent spindle shaped BMMSCs with their in vitro growth characteristics according to El-Ghanayem et al [24]. Also, BMMSCs were identi ed by their adherence to the plastic surfaces of tissue culture asks when maintained under standard culture conditions.…”

Section: Morphological Examination Of Bm-mscsmentioning

confidence: 99%

Ameliorative Effects of Bone Marrow-derived Mesenchymal Stem Cells and Hyaluronic Acid on Monosodium Iodoacetate-Induced Ankle Osteoarthritis in Wistar Rats: Roles of Th1/Th2/Th17 cytokines, MMP-13, TGF-β1 and antioxidant defense system

M.,

Al-Muzafar

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: There is currently no cure for osteoarthritis [OA], and the few pharmacological therapy options available have drawbacks. Additionally, there is no effective treatment that would guarantee a full recovery from OA. Therefore, the goal of this investigation was to determine if bone marrow mesenchymal stem cells [BMMSCs] and/or hyaluronic acid [HA] were effective in treating experimentally-induced ankle osteoarthritis in Wistar rats caused by monosodium iodoacetate [MIA]. Methods: In five groups of ten rats each, fifty male Wistar rats were distributed. Saline was injected into the right tibiotarsal joint of individuals in group I on two days in a row, whereas those in group II received an injection of 2 mg of MIA [dissolved in saline] into the same joint. Those in groups III [MIA-BMMSCs], IV [MIA-HA], and V [MIA-BMMSCs+HA] were injected with MIA similarly to those in group II and also received intra-articular injections of BMMSCs [1×106 cells/rat], HA [75 µg/rat], and BMMSCs [1×106 cells/rat] alongside HA [75 µg/rat] respectively, in the tibiotarsal joint at the ends of the 2nd, 3rd and 4th weeks after MIA injection. The leg circumference, arthritis score, and morphological changes of the ankle joint were evaluated weekly from the beginning to the end of the experiment. For the purpose of identifying oxidative stress, antioxidant, and inflammatory state indicators, sera were collected. For histological and molecular research, the ankles of the right hind leg were excised out and fixed in neutral buffered formalin. Results: In addition to raising GSH and GST levels, BMMSCs and HA decreased serum LPO. Additionally, BMMSC and HA significantly decreased ankle MMP-13 mRNA and TGF-β1 protein expressions as well as TNF-α and IL-17 serum levels. Rats with MIA-induced OA had higher serum IL-4 levels after receiving BMMSC and HA. BMMSC and HA caused a steady decrease in joint injury and cartilage degradation, according to histological investigations. Conclusion: Based on the findings, it is possible to infer that BMMSC and/or HA have anti-arthritic effects that may be mediated by the augmentation of the antioxidant and anti-inflammatory effects in Wistar rats with MIA-induced osteoarthritis. The suppression of MMP-13 and TGF-β1 expressions play an important role in the improvement effects BMMSC and/or HA on OA in Wistar rats.

show abstract

Section: Morphological Examination Of Bm-mscsmentioning

confidence: 99%