The Role of Bile Pigments in Health and Disease: Effects on Cell Signaling, Cytotoxicity, and Cytoprotection

Kapitulnik, Jaime; Maines, Mahin D.

doi:10.3389/fphar.2012.00136

Cited by 19 publications

(17 citation statements)

References 19 publications

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“…In contrast to plant and cyanobacterial phytochromes, BphPs utilize the most far-red absorbing bilin, biliverdin IXα (BV) [8-10]. Being an enzymatic product of heme degradation (Figure 1c), BV is ubiquitous in many eukaryotic organisms including flies, fishes and mammals, unlike tetrapyrrole chromophores of all other phytochrome types [11]. This important feature makes BphP applications in live mammalian cells, tissues and whole mammals as easy as conventional GFP-like FPs, requiring no enzymes or exogenous cofactors [12].…”

Section: Introductionmentioning

confidence: 99%

Near-infrared fluorescent proteins engineered from bacterial phytochromes

Shcherbakova

Baloban

Verkhusha

2015

Current Opinion in Chemical Biology

116

131

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Near-infrared fluorescent proteins (NIR FPs), photoactivatable NIR FPs and NIR reporters of protein-protein interactions developed from bacterial phytochrome photoreceptors (BphPs) have advanced non-invasive deep-tissue imaging. Here we provide a brief guide to the BphP-derived NIR probes with an emphasis on their in vivo applications. We describe phenotypes of NIR FPs and their photochemical and intracellular properties. We discuss NIR FP applications for imaging of various cell types, tissues and animal models in basic and translational research. In this discussion, we focus on NIR FPs that efficiently incorporate endogenous biliverdin chromophore and therefore can be used as straightforward as GFP-like proteins. We also overview a usage of NIR FPs in different imaging platforms, from planar epifluorescence to tomographic and photoacoustic technologies.

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Section: Introductionmentioning

confidence: 99%

Near-infrared fluorescent proteins engineered from bacterial phytochromes

Shcherbakova

Baloban

Verkhusha

2015

Current Opinion in Chemical Biology

116

131

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“…Importantly, BphPs incorporate the most near-infrared-absorbing bilin, biliverdin IXα (BV) (Bhoo et al, 2001), unlike plant and cyanobacterial phytochromes, which utilize phytochromobilin (PΦB) and phycocyanobilin (PCB) (Rockwell and Lagarias, 2010). BV happens to be naturally present in cells and tissues of higher animals, including flies, fishes, and mammals, as a product of enzymatic heme degradation (Kapitulnik and Maines, 2012; Shu et al, 2009; Tran et al, 2014). …”

Section: Introductionmentioning

confidence: 99%

Molecular Basis of Spectral Diversity in Near-Infrared Phytochrome-Based Fluorescent Proteins

Shcherbakova

Baloban

Pletnev

et al. 2015

Chemistry & Biology

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SUMMARY Near-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes (BphPs) are the probes of choice for deep-tissue imaging. Detection of several processes requires spectrally distinct NIR FPs. We developed an NIR FP, BphP1-FP, which has the most blue-shifted spectra and the highest fluorescence quantum yield among BphP-derived FPs. We found that these properties result from the binding of the biliverdin chromophore to a cysteine residue in the GAF domain, unlike natural BphPs and other BphP-based FPs. To elucidate the molecular basis of the spectral shift, we applied biochemical, structural and mass spectrometry analyses and revealed the formation of unique chromophore species. Mutagenesis of NIR FPs of different origins indicated that the mechanism of the spectral shift is general and can be used to design multicolor NIR FPs from other BphPs. We applied pairs of spectrally distinct point cysteine mutants to multicolor cell labeling and demonstrated that they perform well in model deep-tissue imaging.

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“…Fortunately, BV is abundant in eukaryotes, including mammals, as an intermediate of haem degradation pathway to bilirubin78. In wild-type BphPs, light absorption results in BV isomerization and conformational changes of the protein backbone, leading to activation of an output effector domain.…”

mentioning

confidence: 99%

Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging

et al. 2016

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Monomeric near-infrared (NIR) fluorescent proteins (FPs) are in high demand as protein tags and components of biosensors for deep-tissue imaging and multicolour microscopy. We report three bright and spectrally distinct monomeric NIR FPs, termed miRFPs, engineered from bacterial phytochrome, which can be used as easily as GFP-like FPs. miRFPs are 2–5-fold brighter in mammalian cells than other monomeric NIR FPs and perform well in protein fusions, allowing multicolour structured illumination microscopy. miRFPs enable development of several types of NIR biosensors, such as for protein–protein interactions, RNA detection, signalling cascades and cell fate. We demonstrate this by engineering the monomeric fluorescence complementation reporters, the IκBα reporter for NF-κB pathway and the cell cycle biosensor for detection of proliferation status of cells in culture and in animals. miRFPs allow non-invasive visualization and detection of biological processes at different scales, from super-resolution microscopy to in vivo imaging, using the same probes.

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The Role of Bile Pigments in Health and Disease: Effects on Cell Signaling, Cytotoxicity, and Cytoprotection

Cited by 19 publications

References 19 publications

Near-infrared fluorescent proteins engineered from bacterial phytochromes

Near-infrared fluorescent proteins engineered from bacterial phytochromes

Molecular Basis of Spectral Diversity in Near-Infrared Phytochrome-Based Fluorescent Proteins

Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging

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