The role of BADH gene in oxidative, salt, and drought stress tolerances of white clover

Abstract

The aim of this study was to confirm the presence of BADH gene and control whether it plays an important role to protect white clover (Trifolium repens L.) against oxidative, salt, and drought stresses. Three genotypes possessing BADH and 1 genotype lacking BADH were selected, considering the expression levels of BADH in response to methyl viologen mediated oxidative stress. The genotypes possessing BADH displayed significantly less ion leakage under oxidative stress treatment, compared to that lacking BADH. In order to test salinity tolerance, the plants were treated with 300 mM NaCl for 2 weeks, and to mimic drought conditions, plants were grown without irrigating for 5 days. Under the salt stress, the plants possessing BADH contained lower malondialdehyde levels and higher chlorophyll contents than that of plants lacking BADH. Similarly, under the drought conditions, the plants possessing BADH were found to have increased levels of glycinebetaine, proline, and high relative water contents than the plant lacking BADH. These findings indicate that the plants having BADH exhibited enhanced tolerance to salt and drought stresses. These enhanced tolerances of plants possessing BADH gene is attributed to its ability to induce glycinebetaine synthesis. Moreover, the plants possessing BADH were found to exhibit higher relative feed value under both stress and normal conditions. These plants could be used as forage crops on marginal areas having salinity or drought problems.

miRNAs involved in drought stress in Italian ryegrass (Loliummultiflorum L.)

“…Drought stress limits plant growth and quality, which eventually affects crop production sustainability (Khandal et al, 2017). Thus, there is a need for tolerant crops that can stand against drought stress without having any quality and yield loss (Demirkol, 2020). In recent studies, stress defense mechanisms can be explained at the molecular level based on stress factors (Kadioglu et al, 2012;Ma et al, 2019).…”

Improved salt-tolerance of transgenic soybean by stable over-expression of AhBADH gene from Atriplex hortensis

“…PRO is a typical compatible osmolyte that can protect subcellular structures and macromolecules in plant cells under osmotic stress (Ashraf and Foolad 2007; Szabados and Savoure 2010). Usually, free proline accumulates in the cytosol of plant cells in response to salt stress(Binzel et al 1987;Ketchum et al 1991;Demirkol 2020). Under salt stress conditions, overexpression of BADH gene in transgenic plants results in enhanced proline content and salt tolerance, suggesting that GB protects some key enzymes that catalyze the synthesis of free PRO…”